Staphylococcus phage StB27

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; unclassified Azeredovirinae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9A0M2|H9A0M2_9CAUD Uncharacterized protein OS=Staphylococcus phage StB27 OX=1147044 PE=4 SV=1
MM1 pKa = 7.82EE2 pKa = 6.43DD3 pKa = 4.01KK4 pKa = 10.73FIQIIPASEE13 pKa = 3.79NLFSKK18 pKa = 10.54SYY20 pKa = 10.08IEE22 pKa = 4.02EE23 pKa = 4.56DD24 pKa = 3.28GVYY27 pKa = 9.78LYY29 pKa = 11.29SPIVCMALTSDD40 pKa = 4.52GDD42 pKa = 3.93IKK44 pKa = 11.01FCDD47 pKa = 3.77MDD49 pKa = 3.86GSGYY53 pKa = 9.61IDD55 pKa = 4.66EE56 pKa = 5.63IDD58 pKa = 2.98TFMVVKK64 pKa = 9.47YY65 pKa = 10.52LPEE68 pKa = 5.45LDD70 pKa = 4.11EE71 pKa = 4.18YY72 pKa = 11.99VEE74 pKa = 4.95LFDD77 pKa = 5.66FEE79 pKa = 5.04KK80 pKa = 10.85EE81 pKa = 3.97

Molecular weight: 9.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9A0K8|H9A0K8_9CAUD Uncharacterized protein OS=Staphylococcus phage StB27 OX=1147044 PE=4 SV=1
MM1 pKa = 7.74PPHH4 pKa = 6.68IQTMMFNHH12 pKa = 6.7FKK14 pKa = 10.46HH15 pKa = 6.32HH16 pKa = 6.44LPEE19 pKa = 5.39ALEE22 pKa = 4.01RR23 pKa = 11.84CRR25 pKa = 11.84KK26 pKa = 9.19KK27 pKa = 10.62EE28 pKa = 3.75KK29 pKa = 9.85EE30 pKa = 3.98RR31 pKa = 11.84KK32 pKa = 8.47EE33 pKa = 4.01KK34 pKa = 10.5EE35 pKa = 3.76RR36 pKa = 11.84EE37 pKa = 3.86QQRR40 pKa = 11.84LANN43 pKa = 3.94

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12463

43

1130

235.2

26.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.817 ± 0.55

0.522 ± 0.086

6.652 ± 0.352

7.695 ± 0.424

3.996 ± 0.218

5.464 ± 0.312

1.741 ± 0.178

7.197 ± 0.479

8.922 ± 0.414

7.615 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.161

6.852 ± 0.366

2.897 ± 0.253

4.285 ± 0.248

4.044 ± 0.23

6.026 ± 0.34

6.299 ± 0.252

6.331 ± 0.248

1.067 ± 0.163

4.164 ± 0.443
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski