Apilactobacillus ozensis DSM 23829 = JCM 17196

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Apilactobacillus; Apilactobacillus ozensis

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1405 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R2ARJ3|A0A0R2ARJ3_9LACO Uncharacterized protein OS=Apilactobacillus ozensis DSM 23829 = JCM 17196 OX=1423781 GN=FD06_GL000100 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 4.5NWNWSLILRR11 pKa = 11.84ITIYY15 pKa = 10.76VGIGFSYY22 pKa = 9.77YY23 pKa = 10.27FRR25 pKa = 11.84YY26 pKa = 9.85KK27 pKa = 9.79NKK29 pKa = 9.86KK30 pKa = 8.93VALQKK35 pKa = 10.86HH36 pKa = 5.28EE37 pKa = 4.31VQSFAHH43 pKa = 5.71TVDD46 pKa = 3.66GYY48 pKa = 11.18EE49 pKa = 3.56ISKK52 pKa = 10.61NVALHH57 pKa = 6.47KK58 pKa = 9.64FTLKK62 pKa = 9.49TLKK65 pKa = 10.46LCSLTDD71 pKa = 3.41ILISGNYY78 pKa = 8.42IDD80 pKa = 4.38YY81 pKa = 10.78HH82 pKa = 7.15LISDD86 pKa = 4.66GPYY89 pKa = 10.7DD90 pKa = 5.78LEE92 pKa = 6.74DD93 pKa = 4.99DD94 pKa = 4.7DD95 pKa = 5.15WDD97 pKa = 3.85KK98 pKa = 11.57EE99 pKa = 4.06YY100 pKa = 11.19DD101 pKa = 3.63KK102 pKa = 10.97RR103 pKa = 11.84KK104 pKa = 9.31HH105 pKa = 6.38AILEE109 pKa = 4.19EE110 pKa = 4.69EE111 pKa = 4.14YY112 pKa = 10.88DD113 pKa = 3.77QYY115 pKa = 11.68LINQNYY121 pKa = 8.88KK122 pKa = 10.63NSYY125 pKa = 8.3QDD127 pKa = 4.71NIIKK131 pKa = 10.57NKK133 pKa = 10.28NNYY136 pKa = 9.38QNDD139 pKa = 3.66LAMQNSEE146 pKa = 4.32TSQSSYY152 pKa = 11.21NALNNYY158 pKa = 9.28DD159 pKa = 4.6LSDD162 pKa = 3.89NYY164 pKa = 11.07LDD166 pKa = 3.78STSDD170 pKa = 5.34DD171 pKa = 3.72EE172 pKa = 6.44LSDD175 pKa = 3.61NDD177 pKa = 4.25VNLDD181 pKa = 3.63YY182 pKa = 11.33DD183 pKa = 3.56WDD185 pKa = 4.38DD186 pKa = 5.69DD187 pKa = 4.62YY188 pKa = 12.21LDD190 pKa = 5.94DD191 pKa = 6.78DD192 pKa = 6.05DD193 pKa = 7.14SNDD196 pKa = 4.6LDD198 pKa = 4.9NDD200 pKa = 4.26DD201 pKa = 6.04DD202 pKa = 5.89LDD204 pKa = 6.07SDD206 pKa = 5.7DD207 pKa = 6.74LDD209 pKa = 5.41DD210 pKa = 5.68DD211 pKa = 3.8WDD213 pKa = 3.96KK214 pKa = 11.9EE215 pKa = 4.12EE216 pKa = 4.8EE217 pKa = 4.06EE218 pKa = 4.36RR219 pKa = 11.84QNEE222 pKa = 4.29VLDD225 pKa = 4.1EE226 pKa = 4.92EE227 pKa = 4.71YY228 pKa = 10.82DD229 pKa = 3.46QYY231 pKa = 11.73LIDD234 pKa = 4.42SDD236 pKa = 3.88NSYY239 pKa = 11.75DD240 pKa = 4.93HH241 pKa = 7.66DD242 pKa = 4.53DD243 pKa = 3.49EE244 pKa = 6.51NYY246 pKa = 10.74

Molecular weight: 29.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R2B1J2|A0A0R2B1J2_9LACO Uncharacterized protein OS=Apilactobacillus ozensis DSM 23829 = JCM 17196 OX=1423781 GN=FD06_GL000310 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.44QPKK8 pKa = 7.75RR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 8.82RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.03GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.57VLANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.07GRR39 pKa = 11.84KK40 pKa = 8.92SLTVV44 pKa = 3.12

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1405

0

1405

433825

38

5909

308.8

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.455 ± 0.197

0.534 ± 0.018

6.044 ± 0.073

5.159 ± 0.087

4.401 ± 0.066

6.091 ± 0.075

1.957 ± 0.029

8.401 ± 0.098

8.331 ± 0.082

9.103 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.668 ± 0.035

7.295 ± 0.109

3.113 ± 0.044

3.593 ± 0.044

3.339 ± 0.052

7.06 ± 0.17

5.089 ± 0.04

6.566 ± 0.063

0.816 ± 0.023

3.965 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski