BtMf-AlphaCoV/AH2011

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Minunacovirus; Miniopterus bat coronavirus 1

Average proteome isoelectric point is 7.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1WJT6|A0A0U1WJT6_9ALPC Envelope small membrane protein OS=BtMf-AlphaCoV/AH2011 OX=1503278 GN=E PE=3 SV=1
MM1 pKa = 7.56FLILCIALCINFVSSNIGCNSNGNLGMYY29 pKa = 9.8RR30 pKa = 11.84LKK32 pKa = 10.88LGLPSDD38 pKa = 3.64VTNAYY43 pKa = 10.11VSGYY47 pKa = 10.12LPSPNAWNCTINSPGTANITFNAKK71 pKa = 9.86AVFISYY77 pKa = 9.44YY78 pKa = 9.83KK79 pKa = 10.21NGRR82 pKa = 11.84EE83 pKa = 3.98VAVGVGNSKK92 pKa = 11.2SNDD95 pKa = 3.45SIGIYY100 pKa = 10.17FNHH103 pKa = 6.91HH104 pKa = 6.3NGNKK108 pKa = 8.3KK109 pKa = 9.15TILRR113 pKa = 11.84VCRR116 pKa = 11.84WRR118 pKa = 11.84KK119 pKa = 9.29YY120 pKa = 11.42AMPTYY125 pKa = 10.67NPTITTNGEE134 pKa = 3.49DD135 pKa = 4.31CYY137 pKa = 11.4INTNLKK143 pKa = 10.46LDD145 pKa = 4.19FVDD148 pKa = 3.85QANHH152 pKa = 6.31SDD154 pKa = 3.58VFGLSWSGDD163 pKa = 3.15RR164 pKa = 11.84LVIYY168 pKa = 10.53LPDD171 pKa = 3.86RR172 pKa = 11.84VTSYY176 pKa = 10.22YY177 pKa = 10.72VPGIGEE183 pKa = 4.46LDD185 pKa = 3.52VVSFRR190 pKa = 11.84CVLKK194 pKa = 8.58EE195 pKa = 4.05TCAHH199 pKa = 5.26QVITKK204 pKa = 9.52PITAIVNTTSDD215 pKa = 3.8GFISGYY221 pKa = 7.72SVCDD225 pKa = 3.37EE226 pKa = 4.17CDD228 pKa = 3.25GFPKK232 pKa = 10.64YY233 pKa = 10.7VFAATEE239 pKa = 4.27GGAIPPNFQFDD250 pKa = 2.88NWFYY254 pKa = 10.18LTNSSTPISGMFTSVQPFNLSCVWPIPVLTSNSLPVHH291 pKa = 6.55FNMSLNKK298 pKa = 9.85GANCNGYY305 pKa = 10.81SNLDD309 pKa = 3.5VGLVDD314 pKa = 5.3AMRR317 pKa = 11.84FSLNFTDD324 pKa = 3.59NQIIRR329 pKa = 11.84TGVISVKK336 pKa = 10.16TGTNVYY342 pKa = 10.38NFSCTNSSVFGDD354 pKa = 3.34ISVIPFGDD362 pKa = 3.57VEE364 pKa = 4.2QTHH367 pKa = 6.22YY368 pKa = 11.34CFINYY373 pKa = 7.01FTNISTGEE381 pKa = 3.95NASQFVGILPPQVKK395 pKa = 9.71EE396 pKa = 3.69FVIMRR401 pKa = 11.84NGDD404 pKa = 3.34FHH406 pKa = 8.25LNGYY410 pKa = 10.11RR411 pKa = 11.84IFSVDD416 pKa = 3.08TVEE419 pKa = 5.22SVIFNISTRR428 pKa = 11.84DD429 pKa = 3.45GRR431 pKa = 11.84DD432 pKa = 2.95FWTVAFANNAEE443 pKa = 4.17VLTEE447 pKa = 4.01INATSIQNLLYY458 pKa = 10.82CNNPVNSIKK467 pKa = 10.55CQQLRR472 pKa = 11.84FNLDD476 pKa = 2.92NGFYY480 pKa = 10.78SHH482 pKa = 6.97TFDD485 pKa = 5.15SADD488 pKa = 3.42NLPRR492 pKa = 11.84TIVRR496 pKa = 11.84LPKK499 pKa = 10.35YY500 pKa = 8.48VTHH503 pKa = 6.86SYY505 pKa = 11.09INVTVGVNFEE515 pKa = 3.98NRR517 pKa = 11.84TLTNYY522 pKa = 10.51LVDD525 pKa = 4.43FAPDD529 pKa = 3.37GTSIGKK535 pKa = 9.9DD536 pKa = 3.13NITTVCVDD544 pKa = 3.1TTTFTTRR551 pKa = 11.84LNVLAFSAFSTAVDD565 pKa = 3.81IQAGTCPFSYY575 pKa = 11.29NNLNNYY581 pKa = 9.99LGFGSLCFSTRR592 pKa = 11.84PNGGCMMSVVARR604 pKa = 11.84GYY606 pKa = 9.89FGEE609 pKa = 4.14FQKK612 pKa = 11.28VGVLYY617 pKa = 11.01VSFTKK622 pKa = 10.63GDD624 pKa = 3.57NVLGVPPDD632 pKa = 3.68NVPTIGVSDD641 pKa = 3.73MSDD644 pKa = 2.75VKK646 pKa = 11.41LNVCTTFTIYY656 pKa = 10.65GHH658 pKa = 5.54TGRR661 pKa = 11.84GIINKK666 pKa = 9.73SNNTFVSGLFYY677 pKa = 10.84TSVTGNLLGFKK688 pKa = 10.45NSTTGEE694 pKa = 4.0IFSISPCQLTTQVAVVSDD712 pKa = 4.41KK713 pKa = 11.04IVGVAAATKK722 pKa = 9.06EE723 pKa = 4.15VKK725 pKa = 10.64LPFDD729 pKa = 3.47THH731 pKa = 6.71VSLGSFYY738 pKa = 11.14YY739 pKa = 10.56HH740 pKa = 6.92FSNSSAEE747 pKa = 4.08LCKK750 pKa = 10.85NPSLMYY756 pKa = 10.57GGLGVCEE763 pKa = 5.02DD764 pKa = 3.57GRR766 pKa = 11.84LVNISRR772 pKa = 11.84SEE774 pKa = 4.02DD775 pKa = 3.45TFVPSAVISGNITIPANFSFVVQPEE800 pKa = 4.31YY801 pKa = 10.43IQIMTKK807 pKa = 9.87PVSVDD812 pKa = 2.99CSVYY816 pKa = 10.26VCNGNPRR823 pKa = 11.84CLQLLTQYY831 pKa = 11.57ASVCRR836 pKa = 11.84TVEE839 pKa = 4.29EE840 pKa = 4.13PLQLNARR847 pKa = 11.84LEE849 pKa = 4.16ALEE852 pKa = 4.37LTSMIIVQDD861 pKa = 3.61KK862 pKa = 9.2TLHH865 pKa = 6.43LGVASNFNDD874 pKa = 3.82TFDD877 pKa = 3.91LTLALPRR884 pKa = 11.84QHH886 pKa = 6.45QSRR889 pKa = 11.84SAIEE893 pKa = 4.54DD894 pKa = 3.65LLFSKK899 pKa = 10.43IVTSGLGTVDD909 pKa = 5.34DD910 pKa = 5.32DD911 pKa = 4.97YY912 pKa = 11.92KK913 pKa = 11.26EE914 pKa = 4.45CAAKK918 pKa = 9.16MANTIAEE925 pKa = 4.49AGCVQYY931 pKa = 11.5YY932 pKa = 9.78NGIMVLPGVVDD943 pKa = 4.33PSLLSQYY950 pKa = 9.05TAALTGAMVLGGVTAAAAIPFSLAVQARR978 pKa = 11.84LNYY981 pKa = 10.03LALQTDD987 pKa = 4.14VLQRR991 pKa = 11.84NQMILAQSFNAAMGNITVAFSGVSNAIQQTAQSLNTISQALNKK1034 pKa = 9.73VEE1036 pKa = 4.27NVVNEE1041 pKa = 3.91QGTALSQLTKK1051 pKa = 10.61QLASNFQAISSSIEE1065 pKa = 3.69DD1066 pKa = 3.97LYY1068 pKa = 11.84NRR1070 pKa = 11.84LDD1072 pKa = 3.6TLEE1075 pKa = 5.38ADD1077 pKa = 3.74QQVDD1081 pKa = 3.46RR1082 pKa = 11.84LITGRR1087 pKa = 11.84LAALNAFVTQQLLRR1101 pKa = 11.84YY1102 pKa = 8.9SEE1104 pKa = 4.28VRR1106 pKa = 11.84ASRR1109 pKa = 11.84QLAQEE1114 pKa = 4.75KK1115 pKa = 10.45INEE1118 pKa = 4.44CVKK1121 pKa = 10.53SQSSRR1126 pKa = 11.84YY1127 pKa = 8.35GFCGNGTHH1135 pKa = 5.87VFSVANAAPDD1145 pKa = 4.45GIMFLHH1151 pKa = 6.91ANLVPTAFIEE1161 pKa = 4.41VSAFAGVCVDD1171 pKa = 3.46GRR1173 pKa = 11.84ALVLRR1178 pKa = 11.84GRR1180 pKa = 11.84DD1181 pKa = 3.0EE1182 pKa = 4.81VLFQKK1187 pKa = 10.66PGTNDD1192 pKa = 3.15YY1193 pKa = 11.03LITPRR1198 pKa = 11.84LLFEE1202 pKa = 4.25PRR1204 pKa = 11.84VPVSADD1210 pKa = 3.25FVEE1213 pKa = 4.87VSSCNVTFINLTINEE1228 pKa = 4.53LPEE1231 pKa = 4.46LLPDD1235 pKa = 4.18YY1236 pKa = 10.98IDD1238 pKa = 3.45VNKK1241 pKa = 9.25TLKK1244 pKa = 10.34EE1245 pKa = 3.88FAATIPNRR1253 pKa = 11.84TEE1255 pKa = 3.59MQLTLNTYY1263 pKa = 9.78NATVLNLTDD1272 pKa = 3.74EE1273 pKa = 4.85VKK1275 pKa = 10.99SLMSQADD1282 pKa = 4.45DD1283 pKa = 3.54LTKK1286 pKa = 10.24IASEE1290 pKa = 4.28LNLTISKK1297 pKa = 10.27INNTLVEE1304 pKa = 4.51LEE1306 pKa = 3.89WLNRR1310 pKa = 11.84VEE1312 pKa = 5.97TYY1314 pKa = 10.4IKK1316 pKa = 9.3WPWYY1320 pKa = 7.96VWLAIAVTLIILVGLMLWCCLATGCCGCCSCLVNSCGDD1358 pKa = 3.59CGGKK1362 pKa = 9.74RR1363 pKa = 11.84LQRR1366 pKa = 11.84YY1367 pKa = 8.14EE1368 pKa = 3.77IEE1370 pKa = 4.3KK1371 pKa = 10.33VHH1373 pKa = 5.94IQQ1375 pKa = 3.19

Molecular weight: 150.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1WHB2|A0A0U1WHB2_9ALPC 3C-like proteinase OS=BtMf-AlphaCoV/AH2011 OX=1503278 PE=3 SV=1
MM1 pKa = 7.75ASVQFEE7 pKa = 4.47DD8 pKa = 3.64TNSRR12 pKa = 11.84GRR14 pKa = 11.84SGRR17 pKa = 11.84IPLSYY22 pKa = 9.62YY23 pKa = 10.63APVRR27 pKa = 11.84VADD30 pKa = 4.65DD31 pKa = 3.83KK32 pKa = 11.82LFLKK36 pKa = 10.66SMPGNGVPKK45 pKa = 10.76GMGGKK50 pKa = 9.77AEE52 pKa = 4.14QIGYY56 pKa = 6.34WTEE59 pKa = 3.6QRR61 pKa = 11.84RR62 pKa = 11.84WRR64 pKa = 11.84MNKK67 pKa = 8.32GQRR70 pKa = 11.84KK71 pKa = 8.92DD72 pKa = 4.4LPSNWHH78 pKa = 6.54FYY80 pKa = 10.83YY81 pKa = 10.82LGTGPHH87 pKa = 6.91ADD89 pKa = 2.92LKK91 pKa = 10.62FRR93 pKa = 11.84EE94 pKa = 4.21RR95 pKa = 11.84QVGVFWVAKK104 pKa = 9.45QGSKK108 pKa = 9.38TEE110 pKa = 4.03PTNLGTRR117 pKa = 11.84NRR119 pKa = 11.84NQQLRR124 pKa = 11.84IPEE127 pKa = 4.11FDD129 pKa = 3.1FTLPDD134 pKa = 3.11SLEE137 pKa = 4.06IVNSDD142 pKa = 2.95SRR144 pKa = 11.84PASRR148 pKa = 11.84SNSRR152 pKa = 11.84SQSKK156 pKa = 10.82SNNQSRR162 pKa = 11.84SNSKK166 pKa = 10.5ARR168 pKa = 11.84DD169 pKa = 3.42QSGTRR174 pKa = 11.84TPKK177 pKa = 10.4NASQNQKK184 pKa = 9.64PKK186 pKa = 10.77KK187 pKa = 9.04NQQEE191 pKa = 4.18DD192 pKa = 3.64LVAAVRR198 pKa = 11.84EE199 pKa = 4.15ALKK202 pKa = 11.01GLGIQQSRR210 pKa = 11.84SSSGKK215 pKa = 8.13STPARR220 pKa = 11.84SKK222 pKa = 11.13SPARR226 pKa = 11.84PSNKK230 pKa = 9.31KK231 pKa = 9.84QLDD234 pKa = 3.49KK235 pKa = 11.31PEE237 pKa = 4.08WKK239 pKa = 9.82RR240 pKa = 11.84VPNRR244 pKa = 11.84TEE246 pKa = 3.54NATTCFGQRR255 pKa = 11.84SVEE258 pKa = 4.2RR259 pKa = 11.84NCGSASVVGMGVEE272 pKa = 4.43APNFPQIAEE281 pKa = 4.98LIPTQAALFFGSRR294 pKa = 11.84VSTKK298 pKa = 10.23EE299 pKa = 3.51VGDD302 pKa = 3.76TVEE305 pKa = 4.23IKK307 pKa = 10.26YY308 pKa = 9.74HH309 pKa = 5.79YY310 pKa = 10.05KK311 pKa = 9.87MSVPKK316 pKa = 9.99TNPNLPYY323 pKa = 10.51FLQQVNAYY331 pKa = 9.7LDD333 pKa = 4.12PNSDD337 pKa = 3.17QPIPKK342 pKa = 9.52KK343 pKa = 10.36PKK345 pKa = 9.69AKK347 pKa = 10.09PEE349 pKa = 3.99EE350 pKa = 4.21VPQLNPAAPVFTPPAQEE367 pKa = 3.78PAVEE371 pKa = 4.41LEE373 pKa = 4.26MVDD376 pKa = 3.64EE377 pKa = 4.78VFDD380 pKa = 4.29IDD382 pKa = 4.1PLGDD386 pKa = 3.55SVAA389 pKa = 3.81

Molecular weight: 43.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

9241

74

6931

1540.2

171.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.558 ± 0.124

3.344 ± 0.558

5.476 ± 0.756

4.145 ± 0.26

5.746 ± 0.386

6.493 ± 0.311

1.84 ± 0.272

5.378 ± 0.576

5.724 ± 0.929

8.311 ± 0.808

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.056 ± 0.241

6.352 ± 0.659

3.528 ± 0.852

2.738 ± 0.769

3.322 ± 0.575

7.359 ± 0.398

5.995 ± 0.728

10.096 ± 0.77

1.115 ± 0.286

4.415 ± 0.389
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski