Paraburkholderia ribeironis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7335 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N7RLZ1|A0A1N7RLZ1_9BURK KAP NTPase domain-containing protein OS=Paraburkholderia ribeironis OX=1247936 GN=BN2475_70104 PE=4 SV=1
MM1 pKa = 7.22RR2 pKa = 11.84HH3 pKa = 5.85LRR5 pKa = 11.84HH6 pKa = 6.55EE7 pKa = 3.92YY8 pKa = 10.22RR9 pKa = 11.84IRR11 pKa = 11.84LVQRR15 pKa = 11.84SASKK19 pKa = 10.4DD20 pKa = 3.64AAWFAAGKK28 pKa = 8.13PSKK31 pKa = 8.87VTRR34 pKa = 11.84HH35 pKa = 5.54PLWMRR40 pKa = 11.84SVAALMATVMYY51 pKa = 7.68FAPAVFLADD60 pKa = 3.3ATAYY64 pKa = 10.36AAVIVDD70 pKa = 3.67PRR72 pKa = 11.84APVPFQPTVTQGSTGAGIVNITAPNAQGLSVNQFQSLSVGAPGLVFNNSRR122 pKa = 11.84MAGTSLIGGPVGANPNLVGRR142 pKa = 11.84TASMILAQVTSTGAQYY158 pKa = 10.95RR159 pKa = 11.84SVLAGPVEE167 pKa = 4.57VFGAPASLIIANPNGVSIPGGMQVTNASSLTLTTGVPQFVTGPGGVTTDD216 pKa = 3.58YY217 pKa = 11.53AGAGALAYY225 pKa = 9.79DD226 pKa = 4.14VRR228 pKa = 11.84SGDD231 pKa = 3.25IAINGAPGNDD241 pKa = 3.7GTPGPGIDD249 pKa = 3.51GTVGNLDD256 pKa = 5.54LIAQTLSISAPLKK269 pKa = 9.86ADD271 pKa = 3.15QRR273 pKa = 11.84VNLIAGNQLVSPTATGAAGTSYY295 pKa = 9.3GTASNGAANNAAVIQTPGGLAIDD318 pKa = 4.38ASQYY322 pKa = 11.18GSVTSGQIYY331 pKa = 10.05IVSTAAGMGVNTQGALAATAGNVTVNTNGDD361 pKa = 3.0ITAGNTFANQNVTLTSAGTTNVFGTGLANQNFTINANGDD400 pKa = 3.29INATGPVSAGQNVSLNAGGDD420 pKa = 3.66LNAASVAANGSADD433 pKa = 4.6LIAGGSMSLGSLSAHH448 pKa = 6.79DD449 pKa = 5.11LDD451 pKa = 5.59IEE453 pKa = 4.3ATNGNLTLGQAITAPGTIRR472 pKa = 11.84AVAGLDD478 pKa = 3.28LTVNGSVQGGGTVGLSATRR497 pKa = 11.84NAAVNGTVTGVGDD510 pKa = 3.66TSVTGITGTSNISGNVQTNGALTVSSAQGTTLGGIVQAQGPVSVIAQNGSITGQGDD566 pKa = 3.57VASSQGSVSLDD577 pKa = 2.8AGQAINLSGSVQVGGSITALAGGDD601 pKa = 3.53ALFGGTLAAPGAISVTTGGDD621 pKa = 3.02ATLGGNATSGSTLTIAAGANASILGTAASVGNMSLTAANGSLSTANNVVTLGTLAASGQQGVNLGGNVYY690 pKa = 10.51SQGNAQISSGAGSVAVAGALSTPGSITVNAAQDD723 pKa = 3.61VTVTGLLNSGGDD735 pKa = 3.55ATITATRR742 pKa = 11.84DD743 pKa = 3.01ANLNVGLDD751 pKa = 3.62VQGTGNATITVGRR764 pKa = 11.84DD765 pKa = 3.05INGSGAVNVANDD777 pKa = 3.73TTLLAGSNIGISGAIQTGNNLAVTAGNNLAVGATTAVGNEE817 pKa = 4.19TLTATAGSATLASDD831 pKa = 3.81ALSGGDD837 pKa = 3.36MAIRR841 pKa = 11.84AAIDD845 pKa = 3.23VTTPGSVQSLGNLNVNAQGGNLSASGRR872 pKa = 11.84VSTAGTATLNAGQNLSLAGQTIVSGDD898 pKa = 3.41ATLTGANITTQGLAVGGNLTATAQNSLDD926 pKa = 3.57TSAGQLNAAFDD937 pKa = 3.98ASAPALSVNGNATLTGANVTTANAVIGGIYY967 pKa = 9.86SATGTTSIMTGGTAAYY983 pKa = 9.72QSNATLAGGTVTNVGTQMAAGDD1005 pKa = 4.06LTVSGSTVTNQGALSSLQTAAVNATDD1031 pKa = 4.7LNNSGSIYY1039 pKa = 9.43GTAANLNVTNSTVNAGALLATNTLALTTASLDD1071 pKa = 3.57NSNGLIFAGDD1081 pKa = 3.82VNSPTSPVGDD1091 pKa = 3.17VTVNVNGGNGTFNNTNGQILAQNGLTLNLPNQVIDD1126 pKa = 4.12PSAATMGMLNGGSAFNLSAQSISNTGAWTLPGTAVTVIAAQGMSNTGTINQGAGTLALNGAVSNAGTIAAQDD1198 pKa = 3.65LTITGSLANQTGALVQANDD1217 pKa = 3.39AFTLNGSGTNMGTVQAVNSLTIAGSGYY1244 pKa = 10.73DD1245 pKa = 3.5NSNGITQAGNSSSAAGSGNVNISLSGDD1272 pKa = 3.4LTNAGGMLTATNDD1285 pKa = 3.91LSITANNVVNSGAGADD1301 pKa = 3.75PGVTTTTTTTVDD1313 pKa = 3.38NPGLALALVVGSDD1326 pKa = 4.63KK1327 pKa = 10.53IDD1329 pKa = 3.76EE1330 pKa = 4.37AATWGSGGVKK1340 pKa = 9.7FCCTVYY1346 pKa = 11.08GLGTNQVTLADD1357 pKa = 4.14ALSVDD1362 pKa = 4.29GVADD1366 pKa = 3.53PTSINTILGPTAAAWKK1382 pKa = 9.88QFSPPVTNGNTVTFVEE1398 pKa = 4.82IPTVTGTSNTGNSIIQNLWFVQTPDD1423 pKa = 3.33NASQAIATITVVLPTATEE1441 pKa = 4.24TTTTTGGTAGGTSVIAAGHH1460 pKa = 5.51NVSINASSLNNQGGKK1475 pKa = 9.86VSAGNDD1481 pKa = 3.15TSVNVQSLNNGGSVYY1496 pKa = 10.36TSTVTDD1502 pKa = 3.79TVDD1505 pKa = 3.02VASIDD1510 pKa = 3.83SFLSQAPSTISLWNTFNGPNGLEE1533 pKa = 4.06GCPFGMAGVCIDD1545 pKa = 3.67PSGIRR1550 pKa = 11.84LAAPGTVTPLSTSSSITVQGAAGQIVAGHH1579 pKa = 7.04DD1580 pKa = 3.83LNLSGGDD1587 pKa = 3.58LVNAGTLAATNDD1599 pKa = 3.76VNISAASFTNQGTSTGTMTTTAGCAAGFSAGCTTLSTTNPNAQTYY1644 pKa = 10.11SYY1646 pKa = 10.64QQINSNVTAGHH1657 pKa = 7.25DD1658 pKa = 3.25IVIAANTVSNTYY1670 pKa = 11.09GNLAAGNSVVIGGAGTTAAAPTQAASVTNTSGAIAAGNDD1709 pKa = 2.93VDD1711 pKa = 5.02INGATLVNTIAAPVQVHH1728 pKa = 5.19QNYY1731 pKa = 7.67GTATPFTGCTSNCEE1745 pKa = 3.93AYY1747 pKa = 9.71IDD1749 pKa = 4.09VQSATPATITANHH1762 pKa = 6.17NVNLTAGSFSNTGSLITALNNVTINATATAGSDD1795 pKa = 3.37NQYY1798 pKa = 11.49LNAYY1802 pKa = 6.39WASDD1806 pKa = 3.46LLHH1809 pKa = 6.7YY1810 pKa = 7.46NTSYY1814 pKa = 10.76VAWGCASNPSLCQTLYY1830 pKa = 11.02GNAYY1834 pKa = 9.78SSSAAQDD1841 pKa = 3.63PAGLPSSVGLPDD1853 pKa = 4.28FVPATIQAGNTLSVNSPTLTNTGNVVGQTVALSGSQLVNGLMDD1896 pKa = 5.37PDD1898 pKa = 4.9VYY1900 pKa = 10.79TPPPVVTGQVISLGPPAVPANVATTVNGSWQVTNANGQAVSVTGSAGLPSNSPIGVQTVGKK1961 pKa = 8.64PLAPLVSQAGAPAGSTVQTIAGQTIPVTYY1990 pKa = 10.0LVNSPASAVTGDD2002 pKa = 4.53LSPAALLAALPANLQPGTTQFYY2024 pKa = 10.09YY2025 pKa = 10.74DD2026 pKa = 5.26PYY2028 pKa = 9.32TQAQQVEE2035 pKa = 4.56QAALQATGKK2044 pKa = 10.51ASFYY2048 pKa = 9.49STTGATDD2055 pKa = 3.2STGQASISNQDD2066 pKa = 3.0TQALYY2071 pKa = 10.66GAALRR2076 pKa = 11.84YY2077 pKa = 9.93AEE2079 pKa = 4.26QNNVALGTQLSAAQLAQINAPMLWYY2104 pKa = 10.39VEE2106 pKa = 4.05QSVPEE2111 pKa = 4.31PGCTVTGSGACPTVQALMPEE2131 pKa = 4.14VLLPQNYY2138 pKa = 10.12AEE2140 pKa = 4.23VSADD2144 pKa = 3.7GEE2146 pKa = 4.08ISGANVALNYY2156 pKa = 10.7ANSILNTGSISAQNLTVNTASLTNEE2181 pKa = 3.73EE2182 pKa = 4.38RR2183 pKa = 11.84STNIGTIYY2191 pKa = 10.78NNVDD2195 pKa = 2.84NGVAVTTGTLVQQGGFMSAMNYY2217 pKa = 10.24DD2218 pKa = 4.6LNAQTIDD2225 pKa = 3.62QIGGALQQINSDD2237 pKa = 3.41GSVNQAATSQMLANLKK2253 pKa = 9.98SQLGSNFTQSTVSNNLNTTVISDD2276 pKa = 3.39GGMGLMTVFEE2286 pKa = 4.25MAIIVAMSVVTAGAASAALAGMQVAAADD2314 pKa = 3.59AGMATLAAGGTIVEE2328 pKa = 4.45ANAAAGILASSAFAAGGVANAAIAGAMAGLVGSAAGDD2365 pKa = 3.7LMSSGTLNFGDD2376 pKa = 4.08VLKK2379 pKa = 10.84GAAIGGLTAGLTDD2392 pKa = 6.4GITLDD2397 pKa = 3.79DD2398 pKa = 3.98SGIGFSLSGSPDD2410 pKa = 3.17SLASLAGVQSVGNSLVPQAGASTAGSLPEE2439 pKa = 3.95QALALAGEE2447 pKa = 4.49ATVQAGVQTAIDD2459 pKa = 4.03GGSFLASLRR2468 pKa = 11.84NSAVSDD2474 pKa = 3.63VAAAGAYY2481 pKa = 10.24AIGNANLEE2489 pKa = 4.28GDD2491 pKa = 4.45FGTGTQQEE2499 pKa = 4.57LEE2501 pKa = 4.44YY2502 pKa = 10.54VAAHH2506 pKa = 6.67AALGCAASAAEE2517 pKa = 4.25GTGCVGGAIGGAVSAALSPSFIGLIDD2543 pKa = 3.84PVANPLDD2550 pKa = 4.22SGQLAALAAFATLAGGGLAALAGVNVQGAATAAQNEE2586 pKa = 4.31ALNNSSVHH2594 pKa = 6.87IGDD2597 pKa = 4.74GKK2599 pKa = 11.16SEE2601 pKa = 4.2TQKK2604 pKa = 11.06EE2605 pKa = 4.09LDD2607 pKa = 3.63EE2608 pKa = 5.77LRR2610 pKa = 11.84AQQAKK2615 pKa = 9.73EE2616 pKa = 3.65KK2617 pKa = 10.3AALSEE2622 pKa = 4.14ATGGQVTDD2630 pKa = 3.92NSAATVPGSSLTAGLGGATNNAPLGLGSTGRR2661 pKa = 11.84AVSNNLQEE2669 pKa = 4.68QLALQQAMSNPAAGVQLPIPLGDD2692 pKa = 4.1SRR2694 pKa = 11.84WPAADD2699 pKa = 2.99GWVKK2703 pKa = 10.62MSQNVNGVEE2712 pKa = 3.49IHH2714 pKa = 5.49YY2715 pKa = 10.01VRR2717 pKa = 11.84NVNSGAVDD2725 pKa = 3.57DD2726 pKa = 5.43FKK2728 pKa = 11.64FKK2730 pKa = 11.12

Molecular weight: 265.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7SF25|A0A1N7SF25_9BURK Uncharacterized protein OS=Paraburkholderia ribeironis OX=1247936 GN=BN2475_570059 PE=4 SV=1
MM1 pKa = 7.64LLPRR5 pKa = 11.84RR6 pKa = 11.84LGRR9 pKa = 11.84LGLLGLRR16 pKa = 11.84RR17 pKa = 11.84PLGRR21 pKa = 11.84RR22 pKa = 11.84SQRR25 pKa = 11.84LSKK28 pKa = 10.7APRR31 pKa = 11.84RR32 pKa = 11.84PLMGSALYY40 pKa = 9.26EE41 pKa = 3.77RR42 pKa = 11.84SMGTRR47 pKa = 11.84RR48 pKa = 11.84KK49 pKa = 10.13RR50 pKa = 11.84PTRR53 pKa = 11.84VNSRR57 pKa = 3.22

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7335

0

7335

2089330

20

3182

284.8

30.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.57 ± 0.048

1.068 ± 0.012

5.376 ± 0.021

5.042 ± 0.032

3.678 ± 0.016

8.001 ± 0.027

2.43 ± 0.013

4.71 ± 0.021

3.108 ± 0.024

10.095 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.015

2.89 ± 0.02

5.098 ± 0.022

3.725 ± 0.021

7.183 ± 0.036

5.829 ± 0.028

5.425 ± 0.024

7.607 ± 0.026

1.374 ± 0.013

2.406 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski