Clostridioides phage phiSemix9P1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0DZY4|A0A1V0DZY4_9CAUD Uncharacterized protein OS=Clostridioides phage phiSemix9P1 OX=1971232 GN=Semix9P1_phi22 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.35KK3 pKa = 9.64QVYY6 pKa = 9.85YY7 pKa = 11.2NSLDD11 pKa = 3.71EE12 pKa = 4.31KK13 pKa = 11.11EE14 pKa = 4.77KK15 pKa = 10.78IIDD18 pKa = 3.88EE19 pKa = 4.23NSNLYY24 pKa = 10.06IIEE27 pKa = 4.15IYY29 pKa = 8.02EE30 pKa = 4.24TLNEE34 pKa = 4.09NYY36 pKa = 9.94LVLSSSPAEE45 pKa = 4.29DD46 pKa = 3.73KK47 pKa = 10.82EE48 pKa = 4.05LSYY51 pKa = 11.7EE52 pKa = 4.01EE53 pKa = 5.16LEE55 pKa = 4.24NEE57 pKa = 4.23LLIITNEE64 pKa = 4.03LQGGLLL70 pKa = 3.62

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0E003|A0A1V0E003_9CAUD Uncharacterized protein OS=Clostridioides phage phiSemix9P1 OX=1971232 GN=Semix9P1_phi41 PE=4 SV=1
MM1 pKa = 7.67IMNKK5 pKa = 10.0KK6 pKa = 10.52NIFNDD11 pKa = 3.34MFLIIYY17 pKa = 7.3PIIILISMFFNSLLHH32 pKa = 5.15EE33 pKa = 4.19QIIRR37 pKa = 11.84FNINKK42 pKa = 8.46LQVMVFILVYY52 pKa = 9.38FLIFALFIYY61 pKa = 10.46KK62 pKa = 9.5FFIKK66 pKa = 10.33KK67 pKa = 9.69EE68 pKa = 4.09SFHH71 pKa = 7.29IISLIIGMFLIIIIQIPQVLLMISHH96 pKa = 6.71NLFSIVYY103 pKa = 8.72GQLQLEE109 pKa = 4.23IFMFGTLLTLYY120 pKa = 9.75IVMIIMYY127 pKa = 8.83YY128 pKa = 10.2KK129 pKa = 10.45KK130 pKa = 9.51NHH132 pKa = 5.24KK133 pKa = 9.64KK134 pKa = 9.87IRR136 pKa = 11.84GG137 pKa = 3.52

Molecular weight: 16.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

15448

43

1129

208.8

23.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.59 ± 0.424

0.887 ± 0.128

5.962 ± 0.197

7.664 ± 0.491

4.324 ± 0.23

5.23 ± 0.453

1.029 ± 0.092

9.852 ± 0.428

10.409 ± 0.375

8.571 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.167

7.289 ± 0.285

2.11 ± 0.185

2.68 ± 0.142

3.23 ± 0.187

7.14 ± 0.366

6.085 ± 0.347

5.353 ± 0.268

0.738 ± 0.068

4.531 ± 0.373
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski