Ralstonia phage phiRSP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345ANU3|A0A345ANU3_9CAUD Putative transcriptional regulator OS=Ralstonia phage phiRSP OX=2201420 PE=4 SV=1
MM1 pKa = 7.25TEE3 pKa = 3.84YY4 pKa = 9.67VTRR7 pKa = 11.84DD8 pKa = 2.83GDD10 pKa = 3.87MADD13 pKa = 4.92LIAFNYY19 pKa = 9.91YY20 pKa = 7.73GTRR23 pKa = 11.84DD24 pKa = 3.67GLVVEE29 pKa = 4.85QLLDD33 pKa = 4.03ANPGLSGLGPLLPAGITIVLPDD55 pKa = 3.96FTLPATQNAIHH66 pKa = 6.7LWDD69 pKa = 3.46

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345ANT2|A0A345ANT2_9CAUD Putative baseplate assembly protein OS=Ralstonia phage phiRSP OX=2201420 PE=4 SV=1
MM1 pKa = 7.26IHH3 pKa = 7.23FDD5 pKa = 4.36IEE7 pKa = 4.35WNDD10 pKa = 3.67LLRR13 pKa = 11.84IGDD16 pKa = 3.84EE17 pKa = 4.21LGASEE22 pKa = 4.43KK23 pKa = 10.48QIHH26 pKa = 6.15LALSRR31 pKa = 11.84ALKK34 pKa = 9.29RR35 pKa = 11.84TASKK39 pKa = 10.79LRR41 pKa = 11.84TLSAKK46 pKa = 9.63GLRR49 pKa = 11.84DD50 pKa = 3.4EE51 pKa = 6.07LEE53 pKa = 4.17LKK55 pKa = 10.44RR56 pKa = 11.84LNLLRR61 pKa = 11.84KK62 pKa = 9.12RR63 pKa = 11.84LKK65 pKa = 10.3SVKK68 pKa = 9.28LRR70 pKa = 11.84KK71 pKa = 9.94GGAEE75 pKa = 4.15GVKK78 pKa = 9.39IWYY81 pKa = 8.94GLNDD85 pKa = 3.75MPVSWFKK92 pKa = 11.19GKK94 pKa = 8.15PTQNKK99 pKa = 9.95DD100 pKa = 2.54GATFRR105 pKa = 11.84NVKK108 pKa = 10.2FPGAFAASSRR118 pKa = 11.84YY119 pKa = 8.08TKK121 pKa = 10.71GKK123 pKa = 8.91TIFKK127 pKa = 8.89RR128 pKa = 11.84TGKK131 pKa = 10.2ARR133 pKa = 11.84LHH135 pKa = 7.31IEE137 pKa = 4.06EE138 pKa = 4.31QLMPVKK144 pKa = 10.56DD145 pKa = 3.64QADD148 pKa = 3.98VFVEE152 pKa = 3.61DD153 pKa = 4.62HH154 pKa = 7.04IFVQVEE160 pKa = 4.27KK161 pKa = 10.67IFWPLFQRR169 pKa = 11.84EE170 pKa = 3.3IEE172 pKa = 4.03ARR174 pKa = 11.84VKK176 pKa = 10.83YY177 pKa = 10.48KK178 pKa = 10.58IGGAA182 pKa = 3.58

Molecular weight: 20.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

7714

69

672

321.4

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.588 ± 0.501

0.778 ± 0.126

6.495 ± 0.235

5.237 ± 0.368

3.137 ± 0.232

8.984 ± 0.291

1.724 ± 0.267

4.771 ± 0.248

4.421 ± 0.481

7.61 ± 0.335

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.593 ± 0.207

3.682 ± 0.3

4.887 ± 0.353

3.98 ± 0.202

6.663 ± 0.349

5.069 ± 0.249

6.132 ± 0.424

7.104 ± 0.328

1.789 ± 0.176

2.359 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski