Lysobacter pythonis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2550 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M2HJR0|A0A3M2HJR0_9GAMM TPR_REGION domain-containing protein OS=Lysobacter pythonis OX=2483112 GN=EBB59_11575 PE=4 SV=1
MM1 pKa = 7.51SEE3 pKa = 4.25SNADD7 pKa = 3.53SVPFRR12 pKa = 11.84TWMCVVCGFIYY23 pKa = 10.68DD24 pKa = 3.91EE25 pKa = 4.34ATGLPEE31 pKa = 6.02DD32 pKa = 5.36GIAPGTRR39 pKa = 11.84WADD42 pKa = 3.45VPEE45 pKa = 4.4TWTCPDD51 pKa = 3.59CGVTKK56 pKa = 10.55VDD58 pKa = 4.8FDD60 pKa = 3.67MQEE63 pKa = 3.41AA64 pKa = 3.59

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M2I0I5|A0A3M2I0I5_9GAMM Malate synthase OS=Lysobacter pythonis OX=2483112 GN=EBB59_06315 PE=4 SV=1
MM1 pKa = 7.89PPTSRR6 pKa = 11.84PVRR9 pKa = 11.84PIRR12 pKa = 11.84PIRR15 pKa = 11.84PVRR18 pKa = 11.84PVRR21 pKa = 11.84PIRR24 pKa = 11.84PIRR27 pKa = 11.84PIRR30 pKa = 11.84PIRR33 pKa = 11.84PIRR36 pKa = 11.84PIRR39 pKa = 11.84PIRR42 pKa = 11.84PVRR45 pKa = 11.84PVRR48 pKa = 11.84PVRR51 pKa = 11.84PVRR54 pKa = 11.84PVRR57 pKa = 11.84PVRR60 pKa = 11.84PVRR63 pKa = 11.84PVRR66 pKa = 11.84PVRR69 pKa = 11.84PVRR72 pKa = 11.84PVRR75 pKa = 11.84PVRR78 pKa = 11.84PVRR81 pKa = 11.84PVRR84 pKa = 11.84PVRR87 pKa = 11.84PVRR90 pKa = 11.84PVRR93 pKa = 11.84PVRR96 pKa = 11.84PLRR99 pKa = 11.84PLRR102 pKa = 11.84PLRR105 pKa = 11.84PFSTNTHH112 pKa = 5.43

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2550

0

2550

850476

28

4263

333.5

36.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.043 ± 0.074

0.807 ± 0.017

5.7 ± 0.037

5.648 ± 0.05

3.361 ± 0.032

8.8 ± 0.06

2.337 ± 0.031

4.353 ± 0.032

2.932 ± 0.045

10.633 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.334 ± 0.022

2.592 ± 0.036

5.438 ± 0.047

3.404 ± 0.034

8.207 ± 0.052

4.903 ± 0.04

4.73 ± 0.04

6.932 ± 0.042

1.555 ± 0.023

2.29 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski