Amino acid dipepetide frequency for Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.7AlaAla: 3.7 ± 0.111	0.414AlaCys: 0.414 ± 0.029	2.412AlaAsp: 2.412 ± 0.07	4.808AlaGlu: 4.808 ± 0.092	3.184AlaPhe: 3.184 ± 0.075	4.493AlaGly: 4.493 ± 0.104	1.112AlaHis: 1.112 ± 0.045	6.353AlaIle: 6.353 ± 0.13	5.754AlaLys: 5.754 ± 0.114	8.164AlaLeu: 8.164 ± 0.15	1.735AlaMet: 1.735 ± 0.052	2.009AlaAsn: 2.009 ± 0.073	2.034AlaPro: 2.034 ± 0.058	1.378AlaGln: 1.378 ± 0.049	3.799AlaArg: 3.799 ± 0.094	2.997AlaSer: 2.997 ± 0.077	2.919AlaThr: 2.919 ± 0.077	4.916AlaVal: 4.916 ± 0.107	0.71AlaTrp: 0.71 ± 0.033	2.897AlaTyr: 2.897 ± 0.078	0.0AlaXaa: 0.0 ± 0.0
Cys	0.258CysAla: 0.258 ± 0.023	0.059CysCys: 0.059 ± 0.012	0.26CysAsp: 0.26 ± 0.019	0.381CysGlu: 0.381 ± 0.028	0.197CysPhe: 0.197 ± 0.019	0.765CysGly: 0.765 ± 0.043	0.142CysHis: 0.142 ± 0.014	0.377CysIle: 0.377 ± 0.026	0.384CysLys: 0.384 ± 0.029	0.447CysLeu: 0.447 ± 0.032	0.137CysMet: 0.137 ± 0.014	0.26CysAsn: 0.26 ± 0.022	0.651CysPro: 0.651 ± 0.034	0.121CysGln: 0.121 ± 0.016	0.282CysArg: 0.282 ± 0.023	0.345CysSer: 0.345 ± 0.024	0.249CysThr: 0.249 ± 0.02	0.315CysVal: 0.315 ± 0.028	0.074CysTrp: 0.074 ± 0.012	0.208CysTyr: 0.208 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	2.621AspAla: 2.621 ± 0.076	0.22AspCys: 0.22 ± 0.023	1.742AspAsp: 1.742 ± 0.056	4.034AspGlu: 4.034 ± 0.085	2.348AspPhe: 2.348 ± 0.064	2.873AspGly: 2.873 ± 0.078	0.611AspHis: 0.611 ± 0.036	4.221AspIle: 4.221 ± 0.078	3.044AspLys: 3.044 ± 0.068	4.427AspLeu: 4.427 ± 0.093	0.94AspMet: 0.94 ± 0.041	1.38AspAsn: 1.38 ± 0.051	2.447AspPro: 2.447 ± 0.069	0.518AspGln: 0.518 ± 0.033	1.984AspArg: 1.984 ± 0.069	1.752AspSer: 1.752 ± 0.055	1.742AspThr: 1.742 ± 0.05	4.237AspVal: 4.237 ± 0.09	0.603AspTrp: 0.603 ± 0.028	2.111AspTyr: 2.111 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	5.508GluAla: 5.508 ± 0.099	0.362GluCys: 0.362 ± 0.027	4.109GluAsp: 4.109 ± 0.091	10.051GluGlu: 10.051 ± 0.181	3.958GluPhe: 3.958 ± 0.078	5.743GluGly: 5.743 ± 0.111	1.167GluHis: 1.167 ± 0.047	8.493GluIle: 8.493 ± 0.125	8.995GluLys: 8.995 ± 0.141	8.72GluLeu: 8.72 ± 0.154	2.026GluMet: 2.026 ± 0.061	3.742GluAsn: 3.742 ± 0.083	2.433GluPro: 2.433 ± 0.066	1.132GluGln: 1.132 ± 0.047	4.741GluArg: 4.741 ± 0.104	3.176GluSer: 3.176 ± 0.075	2.893GluThr: 2.893 ± 0.084	7.731GluVal: 7.731 ± 0.123	1.148GluTrp: 1.148 ± 0.05	3.132GluTyr: 3.132 ± 0.065	0.0GluXaa: 0.0 ± 0.0
Phe	2.85PheAla: 2.85 ± 0.078	0.22PheCys: 0.22 ± 0.019	2.088PheAsp: 2.088 ± 0.057	3.621PheGlu: 3.621 ± 0.086	1.995PhePhe: 1.995 ± 0.069	3.615PheGly: 3.615 ± 0.072	0.672PheHis: 0.672 ± 0.034	3.42PheIle: 3.42 ± 0.082	2.857PheLys: 2.857 ± 0.069	5.006PheLeu: 5.006 ± 0.117	0.975PheMet: 0.975 ± 0.047	1.453PheAsn: 1.453 ± 0.05	1.967PhePro: 1.967 ± 0.057	0.705PheGln: 0.705 ± 0.032	2.107PheArg: 2.107 ± 0.065	2.786PheSer: 2.786 ± 0.078	1.996PheThr: 1.996 ± 0.067	3.338PheVal: 3.338 ± 0.08	0.592PheTrp: 0.592 ± 0.031	1.853PheTyr: 1.853 ± 0.058	0.0PheXaa: 0.0 ± 0.0
Gly	4.476GlyAla: 4.476 ± 0.106	0.556GlyCys: 0.556 ± 0.032	3.288GlyAsp: 3.288 ± 0.087	5.819GlyGlu: 5.819 ± 0.097	3.221GlyPhe: 3.221 ± 0.081	4.791GlyGly: 4.791 ± 0.098	1.096GlyHis: 1.096 ± 0.045	7.43GlyIle: 7.43 ± 0.112	6.438GlyLys: 6.438 ± 0.112	6.491GlyLeu: 6.491 ± 0.11	1.752GlyMet: 1.752 ± 0.057	2.502GlyAsn: 2.502 ± 0.071	1.989GlyPro: 1.989 ± 0.067	1.157GlyGln: 1.157 ± 0.044	3.754GlyArg: 3.754 ± 0.083	3.363GlySer: 3.363 ± 0.081	3.316GlyThr: 3.316 ± 0.092	5.955GlyVal: 5.955 ± 0.115	0.916GlyTrp: 0.916 ± 0.044	3.227GlyTyr: 3.227 ± 0.08	0.0GlyXaa: 0.0 ± 0.0
His	1.018HisAla: 1.018 ± 0.045	0.137HisCys: 0.137 ± 0.015	0.622HisAsp: 0.622 ± 0.035	1.051HisGlu: 1.051 ± 0.045	0.689HisPhe: 0.689 ± 0.038	1.259HisGly: 1.259 ± 0.05	0.339HisHis: 0.339 ± 0.026	1.186HisIle: 1.186 ± 0.048	0.866HisLys: 0.866 ± 0.035	1.493HisLeu: 1.493 ± 0.052	0.345HisMet: 0.345 ± 0.024	0.457HisAsn: 0.457 ± 0.028	1.011HisPro: 1.011 ± 0.046	0.277HisGln: 0.277 ± 0.024	0.774HisArg: 0.774 ± 0.034	0.779HisSer: 0.779 ± 0.041	0.668HisThr: 0.668 ± 0.033	1.169HisVal: 1.169 ± 0.046	0.206HisTrp: 0.206 ± 0.018	0.777HisTyr: 0.777 ± 0.042	0.0HisXaa: 0.0 ± 0.0
Ile	6.42IleAla: 6.42 ± 0.109	0.481IleCys: 0.481 ± 0.03	3.875IleAsp: 3.875 ± 0.086	7.75IleGlu: 7.75 ± 0.148	3.631IlePhe: 3.631 ± 0.097	5.944IleGly: 5.944 ± 0.113	1.257IleHis: 1.257 ± 0.053	7.385IleIle: 7.385 ± 0.136	6.964IleLys: 6.964 ± 0.109	8.64IleLeu: 8.64 ± 0.153	1.846IleMet: 1.846 ± 0.058	2.821IleAsn: 2.821 ± 0.065	4.291IlePro: 4.291 ± 0.078	1.52IleGln: 1.52 ± 0.053	4.545IleArg: 4.545 ± 0.09	4.791IleSer: 4.791 ± 0.098	4.272IleThr: 4.272 ± 0.091	6.992IleVal: 6.992 ± 0.109	0.774IleTrp: 0.774 ± 0.04	3.047IleTyr: 3.047 ± 0.07	0.0IleXaa: 0.0 ± 0.0
Lys	5.941LysAla: 5.941 ± 0.111	0.519LysCys: 0.519 ± 0.036	3.745LysAsp: 3.745 ± 0.089	8.413LysGlu: 8.413 ± 0.139	3.333LysPhe: 3.333 ± 0.073	5.264LysGly: 5.264 ± 0.104	1.089LysHis: 1.089 ± 0.038	7.369LysIle: 7.369 ± 0.133	6.819LysLys: 6.819 ± 0.132	7.618LysLeu: 7.618 ± 0.128	1.695LysMet: 1.695 ± 0.05	2.847LysAsn: 2.847 ± 0.08	3.212LysPro: 3.212 ± 0.081	1.125LysGln: 1.125 ± 0.049	4.621LysArg: 4.621 ± 0.093	3.13LysSer: 3.13 ± 0.076	3.305LysThr: 3.305 ± 0.074	6.985LysVal: 6.985 ± 0.123	0.918LysTrp: 0.918 ± 0.045	3.032LysTyr: 3.032 ± 0.074	0.0LysXaa: 0.0 ± 0.0
Leu	7.785LeuAla: 7.785 ± 0.131	0.424LeuCys: 0.424 ± 0.028	4.5LeuAsp: 4.5 ± 0.092	8.716LeuGlu: 8.716 ± 0.136	3.873LeuPhe: 3.873 ± 0.099	7.93LeuGly: 7.93 ± 0.12	1.392LeuHis: 1.392 ± 0.054	7.747LeuIle: 7.747 ± 0.13	8.415LeuLys: 8.415 ± 0.129	9.927LeuLeu: 9.927 ± 0.172	2.395LeuMet: 2.395 ± 0.073	3.562LeuAsn: 3.562 ± 0.081	4.353LeuPro: 4.353 ± 0.082	1.841LeuGln: 1.841 ± 0.059	5.516LeuArg: 5.516 ± 0.105	6.226LeuSer: 6.226 ± 0.132	4.836LeuThr: 4.836 ± 0.102	6.848LeuVal: 6.848 ± 0.115	1.129LeuTrp: 1.129 ± 0.04	3.622LeuTyr: 3.622 ± 0.086	0.0LeuXaa: 0.0 ± 0.0
Met	1.699MetAla: 1.699 ± 0.056	0.123MetCys: 0.123 ± 0.013	0.914MetAsp: 0.914 ± 0.038	1.918MetGlu: 1.918 ± 0.066	0.914MetPhe: 0.914 ± 0.038	1.631MetGly: 1.631 ± 0.057	0.346MetHis: 0.346 ± 0.026	1.875MetIle: 1.875 ± 0.061	2.306MetLys: 2.306 ± 0.058	2.275MetLeu: 2.275 ± 0.062	0.499MetMet: 0.499 ± 0.035	0.819MetAsn: 0.819 ± 0.033	0.992MetPro: 0.992 ± 0.039	0.383MetGln: 0.383 ± 0.025	1.347MetArg: 1.347 ± 0.05	1.087MetSer: 1.087 ± 0.044	0.912MetThr: 0.912 ± 0.042	1.558MetVal: 1.558 ± 0.056	0.213MetTrp: 0.213 ± 0.02	0.674MetTyr: 0.674 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	2.337AsnAla: 2.337 ± 0.065	0.258AsnCys: 0.258 ± 0.023	1.196AsnAsp: 1.196 ± 0.049	2.647AsnGlu: 2.647 ± 0.068	1.789AsnPhe: 1.789 ± 0.051	2.589AsnGly: 2.589 ± 0.074	0.44AsnHis: 0.44 ± 0.03	3.042AsnIle: 3.042 ± 0.068	2.007AsnLys: 2.007 ± 0.066	3.828AsnLeu: 3.828 ± 0.079	0.729AsnMet: 0.729 ± 0.033	1.141AsnAsn: 1.141 ± 0.048	2.493AsnPro: 2.493 ± 0.061	0.528AsnGln: 0.528 ± 0.031	1.489AsnArg: 1.489 ± 0.053	1.778AsnSer: 1.778 ± 0.067	1.408AsnThr: 1.408 ± 0.046	3.111AsnVal: 3.111 ± 0.077	0.48AsnTrp: 0.48 ± 0.028	1.515AsnTyr: 1.515 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	2.175ProAla: 2.175 ± 0.061	0.274ProCys: 0.274 ± 0.023	1.901ProAsp: 1.901 ± 0.057	4.299ProGlu: 4.299 ± 0.1	1.882ProPhe: 1.882 ± 0.055	3.049ProGly: 3.049 ± 0.078	0.838ProHis: 0.838 ± 0.035	3.454ProIle: 3.454 ± 0.079	3.241ProLys: 3.241 ± 0.081	4.114ProLeu: 4.114 ± 0.088	0.867ProMet: 0.867 ± 0.04	1.643ProAsn: 1.643 ± 0.055	1.915ProPro: 1.915 ± 0.062	0.959ProGln: 0.959 ± 0.041	2.258ProArg: 2.258 ± 0.059	2.133ProSer: 2.133 ± 0.063	2.232ProThr: 2.232 ± 0.063	3.15ProVal: 3.15 ± 0.076	0.577ProTrp: 0.577 ± 0.032	1.936ProTyr: 1.936 ± 0.055	0.0ProXaa: 0.0 ± 0.0
Gln	1.271GlnAla: 1.271 ± 0.047	0.1GlnCys: 0.1 ± 0.014	0.722GlnAsp: 0.722 ± 0.035	1.693GlnGlu: 1.693 ± 0.056	0.658GlnPhe: 0.658 ± 0.036	1.248GlnGly: 1.248 ± 0.055	0.256GlnHis: 0.256 ± 0.021	1.451GlnIle: 1.451 ± 0.061	1.387GlnLys: 1.387 ± 0.055	1.69GlnLeu: 1.69 ± 0.058	0.521GlnMet: 0.521 ± 0.028	0.613GlnAsn: 0.613 ± 0.036	0.601GlnPro: 0.601 ± 0.03	0.421GlnGln: 0.421 ± 0.03	1.151GlnArg: 1.151 ± 0.051	0.625GlnSer: 0.625 ± 0.035	0.798GlnThr: 0.798 ± 0.04	1.356GlnVal: 1.356 ± 0.05	0.222GlnTrp: 0.222 ± 0.02	0.674GlnTyr: 0.674 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	3.458ArgAla: 3.458 ± 0.096	0.348ArgCys: 0.348 ± 0.026	2.45ArgAsp: 2.45 ± 0.069	6.076ArgGlu: 6.076 ± 0.113	2.199ArgPhe: 2.199 ± 0.06	3.858ArgGly: 3.858 ± 0.092	0.743ArgHis: 0.743 ± 0.033	4.826ArgIle: 4.826 ± 0.091	4.746ArgLys: 4.746 ± 0.107	4.448ArgLeu: 4.448 ± 0.092	1.25ArgMet: 1.25 ± 0.048	1.861ArgAsn: 1.861 ± 0.062	1.801ArgPro: 1.801 ± 0.058	0.89ArgGln: 0.89 ± 0.041	3.498ArgArg: 3.498 ± 0.086	1.92ArgSer: 1.92 ± 0.055	1.979ArgThr: 1.979 ± 0.055	4.183ArgVal: 4.183 ± 0.092	0.684ArgTrp: 0.684 ± 0.034	1.924ArgTyr: 1.924 ± 0.062	0.0ArgXaa: 0.0 ± 0.0
Ser	2.871SerAla: 2.871 ± 0.067	0.348SerCys: 0.348 ± 0.021	1.998SerAsp: 1.998 ± 0.061	3.823SerGlu: 3.823 ± 0.083	2.346SerPhe: 2.346 ± 0.072	3.799SerGly: 3.799 ± 0.08	0.793SerHis: 0.793 ± 0.039	4.136SerIle: 4.136 ± 0.098	3.563SerLys: 3.563 ± 0.081	5.555SerLeu: 5.555 ± 0.118	1.177SerMet: 1.177 ± 0.045	1.614SerAsn: 1.614 ± 0.049	2.247SerPro: 2.247 ± 0.06	1.183SerGln: 1.183 ± 0.053	2.464SerArg: 2.464 ± 0.062	2.938SerSer: 2.938 ± 0.086	2.374SerThr: 2.374 ± 0.068	3.241SerVal: 3.241 ± 0.076	0.653SerTrp: 0.653 ± 0.038	2.121SerTyr: 2.121 ± 0.073	0.0SerXaa: 0.0 ± 0.0
Thr	2.798ThrAla: 2.798 ± 0.074	0.3ThrCys: 0.3 ± 0.023	1.609ThrAsp: 1.609 ± 0.055	2.637ThrGlu: 2.637 ± 0.07	2.223ThrPhe: 2.223 ± 0.057	3.375ThrGly: 3.375 ± 0.081	0.764ThrHis: 0.764 ± 0.038	3.863ThrIle: 3.863 ± 0.084	2.909ThrLys: 2.909 ± 0.07	5.118ThrLeu: 5.118 ± 0.108	0.916ThrMet: 0.916 ± 0.042	1.428ThrAsn: 1.428 ± 0.053	2.651ThrPro: 2.651 ± 0.09	0.985ThrGln: 0.985 ± 0.045	1.989ThrArg: 1.989 ± 0.054	2.393ThrSer: 2.393 ± 0.068	2.575ThrThr: 2.575 ± 0.091	3.352ThrVal: 3.352 ± 0.086	0.558ThrTrp: 0.558 ± 0.029	1.728ThrTyr: 1.728 ± 0.057	0.0ThrXaa: 0.0 ± 0.0
Val	5.233ValAla: 5.233 ± 0.1	0.436ValCys: 0.436 ± 0.03	3.878ValAsp: 3.878 ± 0.08	7.127ValGlu: 7.127 ± 0.142	3.349ValPhe: 3.349 ± 0.078	5.492ValGly: 5.492 ± 0.108	1.198ValHis: 1.198 ± 0.045	6.571ValIle: 6.571 ± 0.103	6.718ValLys: 6.718 ± 0.114	7.475ValLeu: 7.475 ± 0.122	1.546ValMet: 1.546 ± 0.051	2.71ValAsn: 2.71 ± 0.074	3.376ValPro: 3.376 ± 0.077	1.38ValGln: 1.38 ± 0.045	4.085ValArg: 4.085 ± 0.083	4.026ValSer: 4.026 ± 0.084	3.461ValThr: 3.461 ± 0.086	6.538ValVal: 6.538 ± 0.117	0.881ValTrp: 0.881 ± 0.039	3.212ValTyr: 3.212 ± 0.081	0.0ValXaa: 0.0 ± 0.0
Trp	0.802TrpAla: 0.802 ± 0.04	0.059TrpCys: 0.059 ± 0.012	0.691TrpAsp: 0.691 ± 0.041	1.209TrpGlu: 1.209 ± 0.043	0.485TrpPhe: 0.485 ± 0.031	0.84TrpGly: 0.84 ± 0.043	0.22TrpHis: 0.22 ± 0.021	1.061TrpIle: 1.061 ± 0.047	1.034TrpLys: 1.034 ± 0.042	1.048TrpLeu: 1.048 ± 0.042	0.35TrpMet: 0.35 ± 0.024	0.492TrpAsn: 0.492 ± 0.032	0.355TrpPro: 0.355 ± 0.029	0.258TrpGln: 0.258 ± 0.022	0.722TrpArg: 0.722 ± 0.03	0.559TrpSer: 0.559 ± 0.029	0.454TrpThr: 0.454 ± 0.032	0.867TrpVal: 0.867 ± 0.044	0.253TrpTrp: 0.253 ± 0.023	0.443TrpTyr: 0.443 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.568TyrAla: 2.568 ± 0.066	0.275TyrCys: 0.275 ± 0.024	1.854TyrAsp: 1.854 ± 0.063	3.184TyrGlu: 3.184 ± 0.077	1.841TyrPhe: 1.841 ± 0.064	2.978TyrGly: 2.978 ± 0.074	0.648TyrHis: 0.648 ± 0.037	2.985TyrIle: 2.985 ± 0.075	2.535TyrLys: 2.535 ± 0.07	4.555TyrLeu: 4.555 ± 0.098	0.784TyrMet: 0.784 ± 0.036	1.454TyrAsn: 1.454 ± 0.051	2.078TyrPro: 2.078 ± 0.059	0.698TyrGln: 0.698 ± 0.036	2.014TyrArg: 2.014 ± 0.058	2.44TyrSer: 2.44 ± 0.065	1.78TyrThr: 1.78 ± 0.059	2.85TyrVal: 2.85 ± 0.074	0.611TyrTrp: 0.611 ± 0.034	1.924TyrTyr: 1.924 ± 0.059	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2045 proteins (577545 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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