Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1)

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; Opitutales; Opitutaceae; Opitutus; Opitutus terrae

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1ZYB9|B1ZYB9_OPITP Uncharacterized protein OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1) OX=452637 GN=Oter_3742 PE=4 SV=1
MM1 pKa = 7.34EE2 pKa = 5.03AAGWSAFCSDD12 pKa = 4.84ADD14 pKa = 3.95CHH16 pKa = 7.8SDD18 pKa = 4.09DD19 pKa = 4.83VDD21 pKa = 3.47RR22 pKa = 11.84APADD26 pKa = 3.35GCNEE30 pKa = 3.96VEE32 pKa = 4.15AGNYY36 pKa = 8.11QNVLPTIKK44 pKa = 9.96PAPATLVVCDD54 pKa = 4.54CFLGGSDD61 pKa = 5.33LDD63 pKa = 4.1SPPDD67 pKa = 4.01DD68 pKa = 4.13GVVCTGTFDD77 pKa = 4.75RR78 pKa = 11.84PRR80 pKa = 11.84DD81 pKa = 3.73WLVSWQFVRR90 pKa = 11.84RR91 pKa = 11.84AAAPAHH97 pKa = 6.55APDD100 pKa = 4.21SLNAA104 pKa = 4.08

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1ZTU4|B1ZTU4_OPITP Beta-phosphoglucomutase family hydrolase OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1) OX=452637 GN=Oter_1593 PE=4 SV=1
MM1 pKa = 7.45LVGLRR6 pKa = 11.84VRR8 pKa = 11.84GPFRR12 pKa = 11.84ARR14 pKa = 11.84SMPPRR19 pKa = 11.84RR20 pKa = 11.84LAAEE24 pKa = 4.37TVALAPAAAATAASALSLFVPAAMGLAGRR53 pKa = 11.84LRR55 pKa = 11.84TAVRR59 pKa = 11.84SAGLRR64 pKa = 11.84RR65 pKa = 11.84LATSVRR71 pKa = 11.84GAWFVARR78 pKa = 11.84RR79 pKa = 11.84AVGFGRR85 pKa = 4.05

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4588

0

4588

1747334

32

14944

380.8

41.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.471 ± 0.066

0.893 ± 0.013

5.056 ± 0.025

5.546 ± 0.034

3.962 ± 0.021

8.017 ± 0.04

2.191 ± 0.02

4.34 ± 0.026

2.966 ± 0.032

10.529 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.796 ± 0.016

2.801 ± 0.031

5.619 ± 0.026

3.488 ± 0.02

7.599 ± 0.047

5.362 ± 0.034

5.813 ± 0.05

7.562 ± 0.038

1.618 ± 0.019

2.37 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski