Spongiibacter sp. IMCC21906

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Spongiibacteraceae; Spongiibacter; unclassified Spongiibacter

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3192 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7M9B3|A0A0F7M9B3_9GAMM N-acetylmuramoyl-L-alanine amidase OS=Spongiibacter sp. IMCC21906 OX=1620392 GN=IMCC21906_03079 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.53KK3 pKa = 10.33LFALSAISSALLLAACGGGGDD24 pKa = 4.54VNINAQNNSTGNGGGGGDD42 pKa = 3.43PGQTNSCASYY52 pKa = 11.19SDD54 pKa = 5.11DD55 pKa = 4.8NGTTRR60 pKa = 11.84QGSLDD65 pKa = 3.65GVNCVYY71 pKa = 9.97GTDD74 pKa = 4.3FVSLTNPIDD83 pKa = 3.49IGPSSRR89 pKa = 11.84VTFVKK94 pKa = 10.35LANNGVHH101 pKa = 5.97VFRR104 pKa = 11.84DD105 pKa = 3.53SLAVGKK111 pKa = 10.39NYY113 pKa = 10.87DD114 pKa = 3.68NDD116 pKa = 4.09ADD118 pKa = 3.75MAAAGISEE126 pKa = 4.66GGDD129 pKa = 3.18GSIIQIEE136 pKa = 4.26AGATLAFQSSDD147 pKa = 2.95DD148 pKa = 3.64YY149 pKa = 11.38MVINRR154 pKa = 11.84GSQIFAEE161 pKa = 4.92GAMNDD166 pKa = 4.45PITITSVSDD175 pKa = 3.35AVEE178 pKa = 3.98NSVDD182 pKa = 4.09PEE184 pKa = 5.02DD185 pKa = 3.73VQQWGGLIINGFGVTNKK202 pKa = 9.82CAYY205 pKa = 8.23TGSVDD210 pKa = 3.98GGDD213 pKa = 4.06LATSDD218 pKa = 3.7CHH220 pKa = 6.83VAAEE224 pKa = 4.59GKK226 pKa = 10.23AGAGQTHH233 pKa = 6.36YY234 pKa = 11.28GGDD237 pKa = 3.7NNEE240 pKa = 4.61DD241 pKa = 3.22NSGVLKK247 pKa = 10.48YY248 pKa = 9.67FIVKK252 pKa = 8.38HH253 pKa = 5.62TGAQVEE259 pKa = 4.61EE260 pKa = 4.35DD261 pKa = 3.71NEE263 pKa = 4.41LNGIAFDD270 pKa = 4.07AVGSEE275 pKa = 4.12TKK277 pKa = 10.3VDD279 pKa = 3.62YY280 pKa = 10.74LQAYY284 pKa = 6.86ATYY287 pKa = 10.64DD288 pKa = 3.26DD289 pKa = 5.04GIEE292 pKa = 4.1MFGGAVNISHH302 pKa = 6.44YY303 pKa = 10.24VALYY307 pKa = 10.98VNDD310 pKa = 4.29DD311 pKa = 4.37SIDD314 pKa = 3.37IDD316 pKa = 3.6EE317 pKa = 5.64GYY319 pKa = 10.62QGTIDD324 pKa = 3.84YY325 pKa = 10.9ALVIQSEE332 pKa = 4.65TDD334 pKa = 3.02GNRR337 pKa = 11.84CVEE340 pKa = 4.01SDD342 pKa = 3.7GVGSYY347 pKa = 10.83DD348 pKa = 3.9GRR350 pKa = 11.84TDD352 pKa = 3.33VADD355 pKa = 5.04LISRR359 pKa = 11.84GLNSRR364 pKa = 11.84ATIKK368 pKa = 10.85NLTCIISPSQVSGKK382 pKa = 10.65ADD384 pKa = 3.07INGADD389 pKa = 3.49GTGTHH394 pKa = 6.55EE395 pKa = 4.37EE396 pKa = 4.44GQGLRR401 pKa = 11.84IRR403 pKa = 11.84EE404 pKa = 3.97AHH406 pKa = 6.36YY407 pKa = 8.43PTISNALVTTAYY419 pKa = 10.44QADD422 pKa = 4.02QAPDD426 pKa = 3.26AANWCLRR433 pKa = 11.84IDD435 pKa = 4.01NEE437 pKa = 4.39GEE439 pKa = 3.82RR440 pKa = 11.84AALEE444 pKa = 4.13GDD446 pKa = 3.77LVIEE450 pKa = 4.02QSIFACQDD458 pKa = 3.03LTDD461 pKa = 5.0GDD463 pKa = 4.79DD464 pKa = 4.04LDD466 pKa = 4.5GTGTTQEE473 pKa = 4.27TFLTDD478 pKa = 2.97SGNQIYY484 pKa = 10.3QSLPEE489 pKa = 5.17GEE491 pKa = 5.15DD492 pKa = 3.23PTSASNADD500 pKa = 3.71LEE502 pKa = 4.45ILNGFYY508 pKa = 10.85SLLVGDD514 pKa = 4.38MVVGGAAATVTPTEE528 pKa = 3.61GRR530 pKa = 11.84AFVGAVTADD539 pKa = 4.1DD540 pKa = 4.12DD541 pKa = 4.17WTAGWAYY548 pKa = 10.57GLDD551 pKa = 3.62PANRR555 pKa = 11.84GQALWFEE562 pKa = 4.62

Molecular weight: 58.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7M1D5|A0A0F7M1D5_9GAMM Acetyltransferase ribosomal protein N-acetylase OS=Spongiibacter sp. IMCC21906 OX=1620392 GN=IMCC21906_00380 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.08RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84KK29 pKa = 9.38LINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.47GRR39 pKa = 11.84ASLSAA44 pKa = 3.83

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3192

0

3192

1018612

39

1878

319.1

35.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.913 ± 0.052

1.086 ± 0.016

5.82 ± 0.037

6.12 ± 0.043

3.81 ± 0.029

7.502 ± 0.041

2.168 ± 0.022

5.615 ± 0.032

4.31 ± 0.035

10.807 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.483 ± 0.023

3.666 ± 0.026

4.384 ± 0.031

4.386 ± 0.037

5.678 ± 0.033

6.602 ± 0.033

4.736 ± 0.031

6.841 ± 0.041

1.289 ± 0.017

2.785 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski