Candidatus Frankia californiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4681 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C3NY74|A0A1C3NY74_9ACTN Uncharacterized protein OS=Candidatus Frankia californiensis OX=1839754 GN=FDG2_2739 PE=4 SV=1
MM1 pKa = 7.65GGFQRR6 pKa = 11.84ACALSDD12 pKa = 3.5LSEE15 pKa = 4.12EE16 pKa = 4.3VPLGVEE22 pKa = 3.56IDD24 pKa = 3.94GEE26 pKa = 4.59PVCLALTGGQVYY38 pKa = 10.39AIRR41 pKa = 11.84DD42 pKa = 3.89EE43 pKa = 4.59CSHH46 pKa = 7.47ADD48 pKa = 3.26VALSQGEE55 pKa = 4.27VEE57 pKa = 4.53DD58 pKa = 5.24AKK60 pKa = 10.85IEE62 pKa = 4.2CWLHH66 pKa = 6.44GSQFDD71 pKa = 4.14LTTGEE76 pKa = 4.3PLGLPATEE84 pKa = 4.42PVPVYY89 pKa = 9.72PVKK92 pKa = 10.38IDD94 pKa = 3.69GDD96 pKa = 4.11DD97 pKa = 3.47VLVDD101 pKa = 3.66VGQSS105 pKa = 2.94

Molecular weight: 11.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C3NSV6|A0A1C3NSV6_9ACTN Uncharacterized protein OS=Candidatus Frankia californiensis OX=1839754 GN=FDG2_0048 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLSARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.66GRR40 pKa = 11.84SEE42 pKa = 3.94LSAA45 pKa = 4.73

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4679

2

4681

1173277

29

4320

250.6

26.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.098 ± 0.06

0.888 ± 0.012

6.138 ± 0.033

5.023 ± 0.039

2.765 ± 0.022

8.998 ± 0.045

2.355 ± 0.02

3.754 ± 0.029

1.704 ± 0.023

10.192 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.773 ± 0.015

1.847 ± 0.019

6.109 ± 0.034

2.823 ± 0.019

8.703 ± 0.046

5.454 ± 0.033

6.167 ± 0.029

8.76 ± 0.038

1.481 ± 0.017

1.969 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski