Hubei diptera virus 17

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLR3|A0A1L3KLR3_9VIRU Uncharacterized protein OS=Hubei diptera virus 17 OX=1922878 PE=4 SV=1
MM1 pKa = 7.65ISRR4 pKa = 11.84MTHH7 pKa = 5.24FNKK10 pKa = 10.38LPSAEE15 pKa = 3.75GFTFNLRR22 pKa = 11.84PAKK25 pKa = 9.76IDD27 pKa = 2.72AWAYY31 pKa = 6.5EE32 pKa = 3.83ACSRR36 pKa = 11.84HH37 pKa = 6.65FGDD40 pKa = 3.74QKK42 pKa = 10.65IKK44 pKa = 10.76GVVEE48 pKa = 3.97TLHH51 pKa = 6.69RR52 pKa = 11.84SSISYY57 pKa = 8.67TAMVDD62 pKa = 3.66DD63 pKa = 4.31LLGYY67 pKa = 7.56QVKK70 pKa = 10.0PSPRR74 pKa = 11.84TEE76 pKa = 3.77LKK78 pKa = 10.5SYY80 pKa = 9.59HH81 pKa = 6.26LAYY84 pKa = 10.52ASVRR88 pKa = 11.84QDD90 pKa = 3.76LNIDD94 pKa = 3.59TPLIPLTMGAVPQRR108 pKa = 11.84EE109 pKa = 4.38DD110 pKa = 3.49FPGNKK115 pKa = 9.65SPGLPYY121 pKa = 9.93KK122 pKa = 10.4QQGFKK127 pKa = 9.96TKK129 pKa = 10.55RR130 pKa = 11.84EE131 pKa = 4.04VIDD134 pKa = 3.6AGKK137 pKa = 9.53LHH139 pKa = 7.13EE140 pKa = 5.72INRR143 pKa = 11.84DD144 pKa = 2.8WQRR147 pKa = 11.84IGFRR151 pKa = 11.84KK152 pKa = 10.15GKK154 pKa = 10.48VEE156 pKa = 4.42LPDD159 pKa = 3.08VCLFARR165 pKa = 11.84AQIARR170 pKa = 11.84KK171 pKa = 9.28GKK173 pKa = 9.44EE174 pKa = 4.07KK175 pKa = 10.25IRR177 pKa = 11.84ATWGYY182 pKa = 8.28PLHH185 pKa = 6.91VYY187 pKa = 10.52LEE189 pKa = 4.39EE190 pKa = 4.27GRR192 pKa = 11.84FFYY195 pKa = 10.28PIMDD199 pKa = 4.54WIKK202 pKa = 10.81SRR204 pKa = 11.84EE205 pKa = 3.92HH206 pKa = 7.19DD207 pKa = 3.77FPIAYY212 pKa = 9.67GFEE215 pKa = 4.1TANGGMSVISEE226 pKa = 3.95MLARR230 pKa = 11.84NPLCKK235 pKa = 10.41YY236 pKa = 10.25ICTDD240 pKa = 2.33WKK242 pKa = 9.83TFDD245 pKa = 3.83KK246 pKa = 10.96SIPPWLIRR254 pKa = 11.84DD255 pKa = 4.04AFAILAEE262 pKa = 4.82CIDD265 pKa = 3.62WSHH268 pKa = 6.49VKK270 pKa = 10.46DD271 pKa = 3.73VEE273 pKa = 4.24GKK275 pKa = 8.45IWSVRR280 pKa = 11.84PGPSKK285 pKa = 10.6IRR287 pKa = 11.84WKK289 pKa = 10.96RR290 pKa = 11.84MIDD293 pKa = 3.61YY294 pKa = 9.39FVSTPIRR301 pKa = 11.84TCKK304 pKa = 10.57GEE306 pKa = 4.02RR307 pKa = 11.84FLVSTGVPSGSCWTNLIDD325 pKa = 5.11SIVNALVTRR334 pKa = 11.84TCFYY338 pKa = 7.64EE339 pKa = 4.16TTGAFPVDD347 pKa = 4.65EE348 pKa = 5.48IYY350 pKa = 11.12LGDD353 pKa = 4.64DD354 pKa = 3.44GVIIARR360 pKa = 11.84GVIDD364 pKa = 5.76LEE366 pKa = 4.31LLALAAKK373 pKa = 10.47SEE375 pKa = 4.28FGLILNTDD383 pKa = 3.03KK384 pKa = 11.4SYY386 pKa = 10.2VTTNPSNVHH395 pKa = 4.86FLGYY399 pKa = 10.05FNYY402 pKa = 10.81GGFPFKK408 pKa = 11.15NQDD411 pKa = 3.12FLIASFIQPEE421 pKa = 4.17HH422 pKa = 5.95TRR424 pKa = 11.84TSVVEE429 pKa = 4.0ACAAALGQMWSGFDD443 pKa = 3.49PLYY446 pKa = 11.06AVIWFNIICDD456 pKa = 3.64LADD459 pKa = 4.48RR460 pKa = 11.84DD461 pKa = 4.1NLTLEE466 pKa = 4.26EE467 pKa = 4.36VQLHH471 pKa = 5.54MRR473 pKa = 11.84SHH475 pKa = 6.33PHH477 pKa = 4.43RR478 pKa = 11.84HH479 pKa = 5.96KK480 pKa = 10.83YY481 pKa = 9.53LNHH484 pKa = 6.16VGINPKK490 pKa = 9.62TITIPTPRR498 pKa = 11.84DD499 pKa = 3.28GLILDD504 pKa = 4.0VLPSRR509 pKa = 11.84SSTYY513 pKa = 8.41TIKK516 pKa = 10.6RR517 pKa = 11.84RR518 pKa = 11.84TYY520 pKa = 10.66DD521 pKa = 3.8VYY523 pKa = 11.47KK524 pKa = 10.33LWQSVGKK531 pKa = 10.1YY532 pKa = 8.09FNN534 pKa = 3.83

Molecular weight: 60.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLR3|A0A1L3KLR3_9VIRU Uncharacterized protein OS=Hubei diptera virus 17 OX=1922878 PE=4 SV=1
MM1 pKa = 7.61SNAQQEE7 pKa = 4.4MFAQFQAFMASKK19 pKa = 10.08DD20 pKa = 3.64AAGTSHH26 pKa = 7.54PVPSTSAHH34 pKa = 5.67RR35 pKa = 11.84NNRR38 pKa = 11.84SWGKK42 pKa = 9.77KK43 pKa = 7.8GKK45 pKa = 10.15SGGGMPRR52 pKa = 11.84TNVKK56 pKa = 10.78ANLPPVDD63 pKa = 3.99TSASFPAPSGKK74 pKa = 10.02YY75 pKa = 8.7LAKK78 pKa = 9.41TASVEE83 pKa = 4.43KK84 pKa = 10.57NDD86 pKa = 3.92PLYY89 pKa = 11.3AEE91 pKa = 5.36AYY93 pKa = 9.54FPAPADD99 pKa = 3.74EE100 pKa = 5.08FRR102 pKa = 11.84QCADD106 pKa = 3.32YY107 pKa = 11.28QDD109 pKa = 4.77IFTGCQGFPLLASEE123 pKa = 4.71IYY125 pKa = 10.44SRR127 pKa = 11.84LKK129 pKa = 10.6SLSPSFTKK137 pKa = 9.83TVPKK141 pKa = 10.42CAVDD145 pKa = 3.54YY146 pKa = 10.27YY147 pKa = 10.72LAVMLYY153 pKa = 10.69ARR155 pKa = 11.84LLLLHH160 pKa = 6.95RR161 pKa = 11.84EE162 pKa = 4.08NGGDD166 pKa = 3.5LSYY169 pKa = 11.59AEE171 pKa = 4.36SDD173 pKa = 3.68FVEE176 pKa = 5.72LIMSGKK182 pKa = 10.4GIATGGYY189 pKa = 7.14TIPKK193 pKa = 9.97SFALYY198 pKa = 10.36LAGFGNTSLPNGRR211 pKa = 11.84DD212 pKa = 3.44LTWRR216 pKa = 11.84MSKK219 pKa = 8.41PTLASAQIEE228 pKa = 4.3IAGRR232 pKa = 11.84ALTIRR237 pKa = 11.84GYY239 pKa = 10.9FGDD242 pKa = 3.46IVSNILRR249 pKa = 11.84YY250 pKa = 9.87SIYY253 pKa = 10.02PSLGVFAQRR262 pKa = 11.84IVQDD266 pKa = 3.87LARR269 pKa = 11.84TEE271 pKa = 4.75SNQAPVRR278 pKa = 11.84WDD280 pKa = 3.43LPVSFRR286 pKa = 11.84LPGHH290 pKa = 7.53PITDD294 pKa = 3.32SCLGCEE300 pKa = 4.79SARR303 pKa = 11.84HH304 pKa = 4.99MSNEE308 pKa = 3.98QVATLYY314 pKa = 10.91DD315 pKa = 3.67AGVTIDD321 pKa = 5.77DD322 pKa = 4.21FGSNNAEE329 pKa = 3.99LHH331 pKa = 4.9VHH333 pKa = 6.08VGLLNAVHH341 pKa = 6.04QKK343 pKa = 11.12LMGIPILQTKK353 pKa = 6.59TLPVSTIGSQGQIPYY368 pKa = 8.7QQVVEE373 pKa = 4.05LHH375 pKa = 5.79TPGLKK380 pKa = 10.27NAVFSGNSILKK391 pKa = 9.0IASSSGYY398 pKa = 10.12LGSSYY403 pKa = 10.91LYY405 pKa = 10.24RR406 pKa = 11.84IKK408 pKa = 10.99NEE410 pKa = 3.79NAPSILRR417 pKa = 11.84NYY419 pKa = 10.22LPLHH423 pKa = 5.85YY424 pKa = 10.41SNTIPADD431 pKa = 2.78IRR433 pKa = 11.84AAMMTAFDD441 pKa = 4.67ASDD444 pKa = 2.99NTLRR448 pKa = 11.84YY449 pKa = 9.7KK450 pKa = 10.57RR451 pKa = 11.84FEE453 pKa = 4.12VVPYY457 pKa = 10.19SASQRR462 pKa = 11.84LEE464 pKa = 3.91QIVLKK469 pKa = 10.67DD470 pKa = 3.41GQAAA474 pKa = 3.34

Molecular weight: 51.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1008

474

534

504.0

56.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.036 ± 1.403

1.488 ± 0.291

5.258 ± 0.555

4.167 ± 0.389

4.663 ± 0.439

6.944 ± 0.295

2.48 ± 0.249

6.548 ± 0.854

5.357 ± 0.622

8.73 ± 0.512

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.083 ± 0.159

4.067 ± 0.385

5.952 ± 0.111

3.373 ± 0.71

5.556 ± 0.472

7.837 ± 1.253

5.655 ± 0.255

5.655 ± 0.255

1.587 ± 0.64

4.563 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski