Burkholderia virus Bcepmigl

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lessievirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6XGE0|I6XGE0_9CAUD Uncharacterized protein OS=Burkholderia virus Bcepmigl OX=1195073 GN=BcepMigl_gp42 PE=4 SV=1
MM1 pKa = 7.59SALLDD6 pKa = 3.6GLAFGFGMGLFLAMPYY22 pKa = 8.68ITGSGQWEE30 pKa = 3.99

Molecular weight: 3.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6WLP7|I6WLP7_9CAUD Uncharacterized protein OS=Burkholderia virus Bcepmigl OX=1195073 GN=BcepMigl_gp49 PE=4 SV=1
MM1 pKa = 7.8KK2 pKa = 10.11IGTVYY7 pKa = 10.71YY8 pKa = 9.91IEE10 pKa = 4.48SAATGRR16 pKa = 11.84LYY18 pKa = 11.1VGSTARR24 pKa = 11.84APRR27 pKa = 11.84QRR29 pKa = 11.84WLEE32 pKa = 3.83HH33 pKa = 5.19LHH35 pKa = 5.01YY36 pKa = 10.6LRR38 pKa = 11.84NGKK41 pKa = 7.8HH42 pKa = 5.69HH43 pKa = 6.74CRR45 pKa = 11.84HH46 pKa = 5.56LQRR49 pKa = 11.84VYY51 pKa = 11.04AKK53 pKa = 10.33HH54 pKa = 6.46GEE56 pKa = 3.98HH57 pKa = 7.43DD58 pKa = 3.73LTFSIVEE65 pKa = 4.19HH66 pKa = 6.89VDD68 pKa = 4.14DD69 pKa = 5.64ILFLLARR76 pKa = 11.84EE77 pKa = 4.12QFHH80 pKa = 6.4IWRR83 pKa = 11.84HH84 pKa = 4.89EE85 pKa = 3.75GRR87 pKa = 11.84VMNGVPVSDD96 pKa = 4.01ALYY99 pKa = 10.19AAHH102 pKa = 6.97AGNRR106 pKa = 11.84GRR108 pKa = 11.84VMPDD112 pKa = 2.89DD113 pKa = 3.42EE114 pKa = 4.34RR115 pKa = 11.84ARR117 pKa = 11.84RR118 pKa = 11.84SAIAKK123 pKa = 7.35GKK125 pKa = 9.31PKK127 pKa = 10.76GPFSEE132 pKa = 4.15EE133 pKa = 3.31HH134 pKa = 5.83KK135 pKa = 10.71AALRR139 pKa = 11.84TAWEE143 pKa = 3.89RR144 pKa = 11.84RR145 pKa = 11.84RR146 pKa = 11.84AARR149 pKa = 11.84VGRR152 pKa = 11.84QSHH155 pKa = 5.61ITNEE159 pKa = 4.04LPTWLAMRR167 pKa = 11.84QQGMTFRR174 pKa = 11.84QIAEE178 pKa = 3.7ATGRR182 pKa = 11.84DD183 pKa = 3.26RR184 pKa = 11.84HH185 pKa = 6.15CIARR189 pKa = 11.84EE190 pKa = 3.72VRR192 pKa = 11.84AAGCQQ197 pKa = 3.14

Molecular weight: 22.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

19835

30

4658

264.5

28.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.133 ± 0.382

0.792 ± 0.189

6.494 ± 0.209

6.443 ± 0.381

3.151 ± 0.161

8.177 ± 0.39

1.881 ± 0.18

4.517 ± 0.222

4.633 ± 0.326

7.225 ± 0.165

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.113

3.665 ± 0.184

5.42 ± 0.175

4.119 ± 0.33

7.169 ± 0.342

4.971 ± 0.24

5.611 ± 0.219

6.186 ± 0.238

1.502 ± 0.131

2.395 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski