Geomicrobium sp. JCM 19038

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillales incertae sedis; Geomicrobium; unclassified Geomicrobium

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4086 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A061P1C4|A0A061P1C4_9BACL D-arabino-3-hexulose 6-phosphate formaldehyde lyase OS=Geomicrobium sp. JCM 19038 OX=1460635 GN=JCM19038_3996 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84GRR4 pKa = 11.84IVRR7 pKa = 11.84YY8 pKa = 10.06SKK10 pKa = 10.14ATALAGLALTATLTISSEE28 pKa = 3.67VDD30 pKa = 2.97AEE32 pKa = 4.02QGEE35 pKa = 4.7VLKK38 pKa = 10.65ILHH41 pKa = 6.49TNDD44 pKa = 2.15IHH46 pKa = 6.95ATFDD50 pKa = 3.61NFGKK54 pKa = 10.45AAGYY58 pKa = 9.49IDD60 pKa = 4.37QVRR63 pKa = 11.84EE64 pKa = 4.02DD65 pKa = 4.31YY66 pKa = 11.67DD67 pKa = 3.51NVLYY71 pKa = 10.96LDD73 pKa = 4.17AGDD76 pKa = 3.93YY77 pKa = 11.25ASGNPVVDD85 pKa = 4.73LNYY88 pKa = 10.0GIPMVEE94 pKa = 4.62AFNEE98 pKa = 4.02ANLDD102 pKa = 3.48VLTIGNHH109 pKa = 4.81EE110 pKa = 4.21FDD112 pKa = 3.97YY113 pKa = 11.34GQDD116 pKa = 3.31HH117 pKa = 7.77LINNMDD123 pKa = 3.58TSTASWLGANVDD135 pKa = 3.81TGSTGVDD142 pKa = 3.04NPEE145 pKa = 4.55PYY147 pKa = 10.92TMIDD151 pKa = 3.35LDD153 pKa = 4.2GVSVAILGITQAPPATAPANVEE175 pKa = 3.85GMTFDD180 pKa = 3.4SDD182 pKa = 4.12YY183 pKa = 11.2AAVAAAYY190 pKa = 9.62EE191 pKa = 4.21EE192 pKa = 4.67EE193 pKa = 4.58LTSEE197 pKa = 4.24ADD199 pKa = 3.51VIIALTHH206 pKa = 5.63IGYY209 pKa = 9.8NEE211 pKa = 3.86DD212 pKa = 3.32RR213 pKa = 11.84QLAEE217 pKa = 4.19DD218 pKa = 3.36VDD220 pKa = 4.35YY221 pKa = 11.18FDD223 pKa = 5.9VIIGGHH229 pKa = 5.08SHH231 pKa = 5.68TTLNSPQIVNGTLIVQTGSNLNNLGEE257 pKa = 4.13LTLAIEE263 pKa = 4.32NNEE266 pKa = 3.86VTEE269 pKa = 4.16LDD271 pKa = 3.62GTLVPVADD279 pKa = 5.02LDD281 pKa = 4.06TMNEE285 pKa = 3.96DD286 pKa = 3.18VQAVIDD292 pKa = 4.34HH293 pKa = 6.51YY294 pKa = 10.9NSEE297 pKa = 4.02MDD299 pKa = 3.42EE300 pKa = 4.21VLGEE304 pKa = 4.19VIGYY308 pKa = 8.59SEE310 pKa = 4.79NGLSRR315 pKa = 11.84DD316 pKa = 3.67GRR318 pKa = 11.84FQQDD322 pKa = 2.91APLGNFWTDD331 pKa = 3.9AMRR334 pKa = 11.84EE335 pKa = 3.85WAEE338 pKa = 3.63ADD340 pKa = 3.59VAFTNNGGIRR350 pKa = 11.84DD351 pKa = 4.66SIPSGDD357 pKa = 3.38VTLNEE362 pKa = 4.69IYY364 pKa = 10.31TIEE367 pKa = 4.22PFANEE372 pKa = 3.24IMTIEE377 pKa = 4.07MTGEE381 pKa = 4.17AIGNVLEE388 pKa = 4.29YY389 pKa = 10.55SYY391 pKa = 11.73SRR393 pKa = 11.84DD394 pKa = 2.8GRR396 pKa = 11.84NQIDD400 pKa = 3.92LQVSGMEE407 pKa = 4.05YY408 pKa = 10.48EE409 pKa = 4.58ILTGMTGQLSDD420 pKa = 3.78VNMTIDD426 pKa = 4.29GSPLEE431 pKa = 4.99DD432 pKa = 3.35EE433 pKa = 4.33EE434 pKa = 5.39TYY436 pKa = 10.23TVAVADD442 pKa = 4.2YY443 pKa = 9.69IGTGGSGYY451 pKa = 9.55EE452 pKa = 4.1FEE454 pKa = 5.5GEE456 pKa = 4.37VIQEE460 pKa = 4.3TVGFMTDD467 pKa = 5.5AMTDD471 pKa = 3.14WARR474 pKa = 11.84TMTDD478 pKa = 2.5QGEE481 pKa = 4.38TLDD484 pKa = 3.86YY485 pKa = 11.28ASGEE489 pKa = 4.29RR490 pKa = 11.84ISITIDD496 pKa = 3.08PSGPIPGTIIGSTEE510 pKa = 3.67TGLYY514 pKa = 9.0SQNKK518 pKa = 8.27AFQDD522 pKa = 3.51VGLGNLYY529 pKa = 9.71TDD531 pKa = 5.4SILDD535 pKa = 3.64DD536 pKa = 3.7TDD538 pKa = 4.59ADD540 pKa = 4.13FAFLNASSVTGQIPPGEE557 pKa = 4.12ITDD560 pKa = 3.93EE561 pKa = 4.1QIEE564 pKa = 4.14ALDD567 pKa = 3.8GFGNEE572 pKa = 3.99VVVVEE577 pKa = 4.44TTGEE581 pKa = 4.07RR582 pKa = 11.84IEE584 pKa = 4.25EE585 pKa = 4.35VILEE589 pKa = 4.01QSNYY593 pKa = 8.84HH594 pKa = 5.89QGVDD598 pKa = 3.8LQVSGLSYY606 pKa = 10.37TLIPEE611 pKa = 4.1EE612 pKa = 4.4SGSGFEE618 pKa = 4.66SVDD621 pKa = 2.81IVLPDD626 pKa = 3.64GSEE629 pKa = 4.09LEE631 pKa = 3.89EE632 pKa = 5.62DD633 pKa = 3.42EE634 pKa = 5.37AYY636 pKa = 10.66VVAYY640 pKa = 10.59NDD642 pKa = 3.69FMHH645 pKa = 6.95SNSFYY650 pKa = 11.23NFGEE654 pKa = 4.35LIEE657 pKa = 4.46EE658 pKa = 4.9KK659 pKa = 10.89GPVWQKK665 pKa = 9.9VVDD668 pKa = 4.1YY669 pKa = 9.73VTEE672 pKa = 3.87QNRR675 pKa = 11.84PINYY679 pKa = 9.62EE680 pKa = 3.45EE681 pKa = 4.4GLRR684 pKa = 11.84ITIEE688 pKa = 4.29GQDD691 pKa = 3.86VEE693 pKa = 4.6PDD695 pKa = 3.44PTGTWTVAEE704 pKa = 4.23ALQNNHH710 pKa = 5.57GEE712 pKa = 4.23RR713 pKa = 11.84AVRR716 pKa = 11.84GYY718 pKa = 10.43IIGSIQNNEE727 pKa = 3.77VVLGIGEE734 pKa = 4.32HH735 pKa = 6.33APSNLLLADD744 pKa = 4.96DD745 pKa = 4.88PNEE748 pKa = 3.98TDD750 pKa = 3.49RR751 pKa = 11.84SNMLPVQLVNQTPIRR766 pKa = 11.84NGLNLVDD773 pKa = 5.58HH774 pKa = 7.54PDD776 pKa = 3.47HH777 pKa = 6.21LQKK780 pKa = 10.82EE781 pKa = 4.71VVITGSLEE789 pKa = 4.21RR790 pKa = 11.84YY791 pKa = 9.22FGTPGMRR798 pKa = 11.84DD799 pKa = 3.26ANHH802 pKa = 6.3FAWVEE807 pKa = 3.81EE808 pKa = 4.34DD809 pKa = 4.81AVSCQYY815 pKa = 9.65DD816 pKa = 3.06TWQQSDD822 pKa = 4.71VYY824 pKa = 10.11TKK826 pKa = 10.88GDD828 pKa = 3.35RR829 pKa = 11.84VTHH832 pKa = 6.34NDD834 pKa = 2.81VVYY837 pKa = 9.71EE838 pKa = 4.16AKK840 pKa = 9.24WWTVGEE846 pKa = 4.06NPEE849 pKa = 4.62NSSEE853 pKa = 3.81WDD855 pKa = 3.17VWQVVADD862 pKa = 4.11CQSDD866 pKa = 3.48EE867 pKa = 4.54GDD869 pKa = 3.76GIKK872 pKa = 10.42NGRR875 pKa = 11.84RR876 pKa = 11.84RR877 pKa = 3.42

Molecular weight: 95.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A061NWW3|A0A061NWW3_9BACL Transcriptional regulator DeoR family OS=Geomicrobium sp. JCM 19038 OX=1460635 GN=JCM19038_2974 PE=4 SV=1
MM1 pKa = 7.38SKK3 pKa = 8.63KK4 pKa = 8.77TFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.02KK15 pKa = 8.47VHH17 pKa = 5.57GFRR20 pKa = 11.84SRR22 pKa = 11.84MSTKK26 pKa = 10.33NGRR29 pKa = 11.84LVLKK33 pKa = 10.39RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 10.33GRR40 pKa = 11.84KK41 pKa = 8.52VLSAA45 pKa = 4.05

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4086

0

4086

1114903

37

1794

272.9

30.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.444 ± 0.037

0.625 ± 0.012

5.485 ± 0.037

7.777 ± 0.051

4.346 ± 0.032

6.874 ± 0.034

2.461 ± 0.022

7.082 ± 0.035

5.165 ± 0.04

9.683 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.914 ± 0.019

3.946 ± 0.026

3.661 ± 0.027

4.044 ± 0.025

4.539 ± 0.028

6.113 ± 0.026

5.915 ± 0.028

7.514 ± 0.032

1.005 ± 0.015

3.407 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski