Bacillus phage BCP8-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Caeruleovirus; Bacillus virus BCP82

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3D9N9|A0A0E3D9N9_9CAUD Putative major capsid OS=Bacillus phage BCP8-2 OX=1129192 GN=BCP8-2_020 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.24IPWEE6 pKa = 4.44CEE8 pKa = 3.77DD9 pKa = 4.59CGHH12 pKa = 6.98KK13 pKa = 10.38EE14 pKa = 3.65LASPFDD20 pKa = 3.77TFMLCPNCDD29 pKa = 3.22SQNFFHH35 pKa = 7.24GSIIEE40 pKa = 4.57DD41 pKa = 3.67DD42 pKa = 3.85LEE44 pKa = 4.33EE45 pKa = 4.42YY46 pKa = 10.58NVYY49 pKa = 10.71DD50 pKa = 5.6EE51 pKa = 5.56EE52 pKa = 6.53DD53 pKa = 3.44EE54 pKa = 4.41LDD56 pKa = 3.62NVVVFEE62 pKa = 4.29DD63 pKa = 3.69QYY65 pKa = 11.74GEE67 pKa = 4.16KK68 pKa = 9.61LTIVQEE74 pKa = 4.01IDD76 pKa = 3.25DD77 pKa = 5.19DD78 pKa = 5.18GDD80 pKa = 3.96INLLPSDD87 pKa = 3.99RR88 pKa = 11.84NLFFSKK94 pKa = 10.41EE95 pKa = 3.88DD96 pKa = 4.04AIEE99 pKa = 3.86LAKK102 pKa = 10.81AILRR106 pKa = 11.84VCGEE110 pKa = 4.15EE111 pKa = 4.02EE112 pKa = 3.81

Molecular weight: 12.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3D9S3|A0A0E3D9S3_9CAUD Putative baseplate protein II OS=Bacillus phage BCP8-2 OX=1129192 GN=BCP8-2_214 PE=4 SV=1
MM1 pKa = 7.59LVQHH5 pKa = 6.04YY6 pKa = 10.37KK7 pKa = 10.89GIRR10 pKa = 11.84LQLINRR16 pKa = 11.84RR17 pKa = 11.84YY18 pKa = 10.33GNRR21 pKa = 11.84KK22 pKa = 9.04AKK24 pKa = 10.31RR25 pKa = 11.84FTLGGTNQNVWIPNKK40 pKa = 10.13HH41 pKa = 6.03LEE43 pKa = 4.11EE44 pKa = 5.72DD45 pKa = 3.95GSIKK49 pKa = 10.38QGEE52 pKa = 4.29NIDD55 pKa = 3.73YY56 pKa = 10.22VFRR59 pKa = 11.84RR60 pKa = 11.84AQRR63 pKa = 11.84QLEE66 pKa = 4.15LAGYY70 pKa = 6.61TEE72 pKa = 5.12PIIGIKK78 pKa = 9.96RR79 pKa = 11.84RR80 pKa = 11.84TIVEE84 pKa = 3.75EE85 pKa = 4.48RR86 pKa = 11.84II87 pKa = 3.59

Molecular weight: 10.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

220

0

220

45866

34

1288

208.5

23.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.819 ± 0.203

0.798 ± 0.067

6.255 ± 0.12

8.01 ± 0.291

3.807 ± 0.11

6.608 ± 0.242

1.84 ± 0.099

6.543 ± 0.134

7.999 ± 0.191

8.01 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.684 ± 0.093

5.335 ± 0.168

3.297 ± 0.14

3.726 ± 0.129

4.489 ± 0.132

5.73 ± 0.16

6.299 ± 0.217

7.003 ± 0.16

1.212 ± 0.074

4.535 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski