Schaalia cardiffensis F0333

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Schaalia; Schaalia cardiffensis

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1983 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N6W604|N6W604_9ACTO Integral membrane protein OS=Schaalia cardiffensis F0333 OX=888050 GN=HMPREF9004_1344 PE=4 SV=1
MM1 pKa = 7.7WIICGGEE8 pKa = 3.94QRR10 pKa = 11.84ISWYY14 pKa = 8.79FTVEE18 pKa = 4.01NVSDD22 pKa = 3.44QTFEE26 pKa = 4.28AFHH29 pKa = 6.46SLSVPDD35 pKa = 4.83PALDD39 pKa = 4.25PYY41 pKa = 9.57PASGVVIPDD50 pKa = 3.54DD51 pKa = 3.84PKK53 pKa = 10.96GFLVVPEE60 pKa = 5.05GII62 pKa = 3.84

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N6WEG3|N6WEG3_9ACTO Hemin ABC superfamily ATP binding cassette transporter membrane protein OS=Schaalia cardiffensis F0333 OX=888050 GN=HMPREF9004_0688 PE=3 SV=1
MM1 pKa = 8.22DD2 pKa = 4.83SRR4 pKa = 11.84PPLSPRR10 pKa = 11.84RR11 pKa = 11.84TAVTTKK17 pKa = 9.88RR18 pKa = 11.84TFQPNNRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84SKK29 pKa = 8.58THH31 pKa = 5.46GFRR34 pKa = 11.84LRR36 pKa = 11.84MSTRR40 pKa = 11.84AGRR43 pKa = 11.84AILAARR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84KK52 pKa = 9.74GRR54 pKa = 11.84AKK56 pKa = 10.68LSAA59 pKa = 3.92

Molecular weight: 6.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1983

0

1983

628407

37

3061

316.9

34.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.674 ± 0.069

0.825 ± 0.017

5.613 ± 0.048

6.811 ± 0.053

3.112 ± 0.036

8.345 ± 0.05

2.147 ± 0.029

5.08 ± 0.041

3.035 ± 0.044

10.158 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.027

2.291 ± 0.029

5.243 ± 0.048

2.992 ± 0.039

6.91 ± 0.064

6.768 ± 0.053

5.687 ± 0.042

7.73 ± 0.054

1.423 ± 0.026

1.925 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski