Bacillus phage Stahl

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Slashvirus; Bacillus virus Stahl

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3JJ23|A0A0E3JJ23_9CAUD Uncharacterized protein OS=Bacillus phage Stahl OX=1610832 GN=CPT_Stahl4 PE=4 SV=1
MM1 pKa = 7.89NEE3 pKa = 3.71EE4 pKa = 5.2LYY6 pKa = 11.18NSACGIVGICNLDD19 pKa = 3.56SEE21 pKa = 5.12EE22 pKa = 4.01EE23 pKa = 4.17LEE25 pKa = 4.42NEE27 pKa = 3.91IKK29 pKa = 10.51EE30 pKa = 4.37LEE32 pKa = 4.22EE33 pKa = 4.44NPDD36 pKa = 3.21FTFFGTFEE44 pKa = 4.07MTQDD48 pKa = 3.11ADD50 pKa = 4.27EE51 pKa = 4.34EE52 pKa = 4.49LKK54 pKa = 10.97HH55 pKa = 6.84QGFSLDD61 pKa = 5.24DD62 pKa = 3.43ITKK65 pKa = 9.78MSLNRR70 pKa = 11.84YY71 pKa = 9.11GNVIEE76 pKa = 5.01CLKK79 pKa = 10.94APNSDD84 pKa = 3.22YY85 pKa = 10.92HH86 pKa = 7.9VYY88 pKa = 10.49VGARR92 pKa = 3.27

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3JQ50|A0A0E3JQ50_9CAUD Uncharacterized protein OS=Bacillus phage Stahl OX=1610832 GN=CPT_Stahl24 PE=4 SV=1
MM1 pKa = 7.63ANHH4 pKa = 6.39YY5 pKa = 9.44KK6 pKa = 10.81NKK8 pKa = 9.08VRR10 pKa = 11.84RR11 pKa = 11.84NFIGNVKK18 pKa = 9.99VFMSDD23 pKa = 2.69HH24 pKa = 5.33AVIRR28 pKa = 11.84FVEE31 pKa = 4.22RR32 pKa = 11.84QNLDD36 pKa = 3.35PAGLDD41 pKa = 3.02INAVRR46 pKa = 11.84TEE48 pKa = 4.4IIRR51 pKa = 11.84KK52 pKa = 8.41FRR54 pKa = 11.84NSKK57 pKa = 10.65LGGFLANGKK66 pKa = 7.02EE67 pKa = 3.76RR68 pKa = 11.84RR69 pKa = 11.84YY70 pKa = 10.1EE71 pKa = 3.89VAGSMNEE78 pKa = 3.67RR79 pKa = 11.84MQFICKK85 pKa = 9.86KK86 pKa = 8.32EE87 pKa = 3.82EE88 pKa = 3.77CGKK91 pKa = 10.58YY92 pKa = 10.66VIISCQLQGKK102 pKa = 9.28RR103 pKa = 11.84NDD105 pKa = 2.61WWKK108 pKa = 11.37NEE110 pKa = 3.66GLVKK114 pKa = 10.67NAA116 pKa = 4.39

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

23866

26

2249

217.0

24.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.352 ± 0.42

0.8 ± 0.132

6.18 ± 0.139

7.442 ± 0.421

4.592 ± 0.2

6.449 ± 0.298

1.676 ± 0.144

6.7 ± 0.196

8.699 ± 0.263

7.643 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.925 ± 0.199

6.126 ± 0.21

2.665 ± 0.188

3.968 ± 0.327

3.985 ± 0.196

5.929 ± 0.27

6.239 ± 0.341

6.532 ± 0.148

1.081 ± 0.075

4.018 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski