Quadrisphaera sp. DSM 44207

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Kineosporiales; Kineosporiaceae; Quadrisphaera; unclassified Quadrisphaera

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2934 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0YHJ9|A0A1H0YHJ9_9ACTN 50S ribosomal protein L7/L12 OS=Quadrisphaera sp. DSM 44207 OX=1881057 GN=rplL PE=3 SV=1
MM1 pKa = 7.59SPRR4 pKa = 11.84LSRR7 pKa = 11.84ATAAGLLACCLGAGALSCTGGTDD30 pKa = 2.83AGTDD34 pKa = 3.12AGTDD38 pKa = 3.12AGTDD42 pKa = 3.21AGTDD46 pKa = 3.58PAATQAPAPDD56 pKa = 4.32GSASAGTTAAAPFGPGCASLPSGGQLPDD84 pKa = 3.51QPLSSALASVPQLSRR99 pKa = 11.84LASAVEE105 pKa = 4.22SAGLGQRR112 pKa = 11.84LDD114 pKa = 4.22DD115 pKa = 4.58LPAGAVFAPSDD126 pKa = 3.73DD127 pKa = 3.82AFASLGISDD136 pKa = 5.24LAALADD142 pKa = 3.74PASRR146 pKa = 11.84GEE148 pKa = 3.79VLQYY152 pKa = 10.82HH153 pKa = 6.06VVPQGLAPEE162 pKa = 4.24VAGTYY167 pKa = 7.49EE168 pKa = 4.13TLQGGTLTVEE178 pKa = 4.7GSGQEE183 pKa = 3.95YY184 pKa = 8.46TVDD187 pKa = 3.56GEE189 pKa = 4.17ASIVCGGIRR198 pKa = 11.84ADD200 pKa = 3.98DD201 pKa = 3.55STIYY205 pKa = 10.89VVDD208 pKa = 4.25ALLTPPRR215 pKa = 4.78

Molecular weight: 20.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0YPE4|A0A1H0YPE4_9ACTN Osmoprotectant transport system permease protein OS=Quadrisphaera sp. DSM 44207 OX=1881057 GN=SAMN05428996_0889 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.64HH17 pKa = 5.77RR18 pKa = 11.84KK19 pKa = 7.85LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84HH26 pKa = 3.6QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2934

0

2934

955155

28

1684

325.5

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

16.261 ± 0.093

0.638 ± 0.012

5.842 ± 0.034

5.476 ± 0.039

2.16 ± 0.027

9.901 ± 0.044

2.008 ± 0.021

1.914 ± 0.033

1.017 ± 0.023

10.878 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.301 ± 0.015

1.105 ± 0.019

6.469 ± 0.041

2.769 ± 0.022

8.771 ± 0.052

4.857 ± 0.032

5.37 ± 0.036

10.335 ± 0.045

1.387 ± 0.017

1.542 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski