Cryobacterium sp. Hh39

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Cryobacterium

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4157 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A5MGS3|A0A3A5MGS3_9MICO 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cryobacterium sp. Hh39 OX=995039 GN=D6T64_07085 PE=3 SV=1
MM1 pKa = 7.43HH2 pKa = 7.25KK3 pKa = 10.21RR4 pKa = 11.84YY5 pKa = 8.74FAAPALVAAAALALSGCASTPASSSAADD33 pKa = 3.29GDD35 pKa = 4.11LRR37 pKa = 11.84VVATTTQIADD47 pKa = 3.79FTRR50 pKa = 11.84SVVGDD55 pKa = 3.55AAGVSITQLVQPNQSLHH72 pKa = 7.06SYY74 pKa = 10.25DD75 pKa = 4.1PSAADD80 pKa = 3.61LTALGAADD88 pKa = 3.34VLVINGVDD96 pKa = 3.6LEE98 pKa = 4.33EE99 pKa = 4.43WLDD102 pKa = 3.8DD103 pKa = 5.27AIDD106 pKa = 3.65ASGFDD111 pKa = 3.98GVTIDD116 pKa = 3.82SDD118 pKa = 3.57EE119 pKa = 5.13GIDD122 pKa = 3.69IIEE125 pKa = 4.42SEE127 pKa = 4.28VDD129 pKa = 3.17EE130 pKa = 4.79HH131 pKa = 9.31AEE133 pKa = 3.94DD134 pKa = 4.43HH135 pKa = 7.19ADD137 pKa = 3.61EE138 pKa = 4.45TDD140 pKa = 3.47EE141 pKa = 3.89EE142 pKa = 4.54HH143 pKa = 7.0AAEE146 pKa = 4.1EE147 pKa = 3.92AAAAAEE153 pKa = 4.3EE154 pKa = 4.58AEE156 pKa = 4.51HH157 pKa = 7.56ADD159 pKa = 3.59EE160 pKa = 4.73TEE162 pKa = 4.16EE163 pKa = 3.79EE164 pKa = 4.37HH165 pKa = 7.27AAEE168 pKa = 5.17DD169 pKa = 4.35DD170 pKa = 4.14GEE172 pKa = 4.23HH173 pKa = 6.2AHH175 pKa = 7.55DD176 pKa = 5.43GGDD179 pKa = 3.36PHH181 pKa = 8.45IWTDD185 pKa = 3.21VNNAMKK191 pKa = 10.03MVGTIAGGLEE201 pKa = 3.98EE202 pKa = 5.29ALSADD207 pKa = 4.04SADD210 pKa = 3.18TAAAIEE216 pKa = 4.39ANATAYY222 pKa = 10.11SQQLSEE228 pKa = 4.3LDD230 pKa = 2.98SWIRR234 pKa = 11.84TNVDD238 pKa = 3.23TVPAAEE244 pKa = 4.28RR245 pKa = 11.84LLVSNHH251 pKa = 6.75DD252 pKa = 3.54ALGYY256 pKa = 7.99YY257 pKa = 7.99TAAYY261 pKa = 9.24GITYY265 pKa = 9.47VGAVIPSFDD274 pKa = 4.64DD275 pKa = 3.41NAEE278 pKa = 4.05PSAAAIDD285 pKa = 4.03EE286 pKa = 4.27LVAAITATGVQAVFSEE302 pKa = 4.5ASLNPKK308 pKa = 8.6TADD311 pKa = 3.72TIASEE316 pKa = 4.6ANVTVYY322 pKa = 10.76SGDD325 pKa = 3.39DD326 pKa = 3.27ALYY329 pKa = 10.66VDD331 pKa = 4.86SLGPDD336 pKa = 3.9GSAGATYY343 pKa = 10.31IAAQLHH349 pKa = 5.33NTTAILEE356 pKa = 4.46SWGVTPTDD364 pKa = 3.4VPADD368 pKa = 3.74LEE370 pKa = 4.37KK371 pKa = 11.14

Molecular weight: 38.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A5MSW1|A0A3A5MSW1_9MICO Uncharacterized protein OS=Cryobacterium sp. Hh39 OX=995039 GN=D6T64_02815 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4157

0

4157

1328963

29

5497

319.7

34.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.315 ± 0.056

0.559 ± 0.01

5.848 ± 0.036

5.11 ± 0.034

3.223 ± 0.022

8.745 ± 0.032

1.982 ± 0.016

4.694 ± 0.033

2.032 ± 0.027

10.447 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.812 ± 0.015

2.357 ± 0.02

5.367 ± 0.031

2.923 ± 0.019

6.65 ± 0.041

6.025 ± 0.029

6.777 ± 0.038

8.736 ± 0.034

1.411 ± 0.016

1.988 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski