Mycoplasma mycoides subsp. mycoides SC (strain PG1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma mycoides group; Mycoplasma mycoides; Mycoplasma mycoides subsp. mycoides; Mycoplasma mycoides subsp. mycoides SC

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 978 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q6MSP2|RS5_MYCMS 30S ribosomal protein S5 OS=Mycoplasma mycoides subsp. mycoides SC (strain PG1) OX=272632 GN=rpsE PE=3 SV=1
MM1 pKa = 7.18YY2 pKa = 10.34LISNLDD8 pKa = 3.55FILIYY13 pKa = 10.15FLSYY17 pKa = 10.42LAVLIWVLDD26 pKa = 3.94FLAIRR31 pKa = 11.84TFLPLIISNPTFVTWSLFFGSCNATFDD58 pKa = 3.01IWSFAGISLIPPSLSSLVGLTCFLTVIPWTEE89 pKa = 3.85THH91 pKa = 6.49CLSFVIEE98 pKa = 4.71VIGPICPLCEE108 pKa = 3.71PAITLTTSPFKK119 pKa = 10.85ILDD122 pKa = 3.52FAINWFLLL130 pKa = 3.81

Molecular weight: 14.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q6MRU5|MNMG_MYCMS tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG OS=Mycoplasma mycoides subsp. mycoides SC (strain PG1) OX=272632 GN=mnmG2 PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.52LKK11 pKa = 10.34HH12 pKa = 6.07AGVHH16 pKa = 5.56GFRR19 pKa = 11.84ARR21 pKa = 11.84MATEE25 pKa = 3.82NGRR28 pKa = 11.84KK29 pKa = 9.16VIKK32 pKa = 10.24ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.58GRR39 pKa = 11.84VRR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

978

0

978

319461

36

1972

326.6

37.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.417 ± 0.068

0.657 ± 0.024

5.647 ± 0.058

5.789 ± 0.076

5.248 ± 0.063

4.337 ± 0.072

1.365 ± 0.025

10.128 ± 0.086

10.863 ± 0.096

9.879 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.747 ± 0.035

8.261 ± 0.096

2.464 ± 0.033

3.678 ± 0.044

2.69 ± 0.039

6.649 ± 0.065

5.393 ± 0.046

5.401 ± 0.065

1.045 ± 0.027

4.342 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski