Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO) (Thermotoga lettingae)

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Thermotogales; Thermotogaceae; Pseudothermotoga; Pseudothermotoga lettingae

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2040 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8F3V2|A8F3V2_PSELT Conserved carboxylase region OS=Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO) OX=416591 GN=Tlet_0266 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.41LKK4 pKa = 10.04TITTLWLIVASILLLSSCAFNLFGDD29 pKa = 4.9LEE31 pKa = 4.38FQSLLSSGTDD41 pKa = 3.35EE42 pKa = 5.15QKK44 pKa = 11.27LEE46 pKa = 4.01NAKK49 pKa = 10.36NALSSKK55 pKa = 10.59DD56 pKa = 3.51YY57 pKa = 10.11TKK59 pKa = 10.92AIALSGSVLNNILGLNLTNEE79 pKa = 4.35QLTSLLDD86 pKa = 3.73STSTLYY92 pKa = 10.78EE93 pKa = 4.3FAQSLYY99 pKa = 10.5EE100 pKa = 5.5KK101 pKa = 10.37KK102 pKa = 10.61DD103 pKa = 3.63EE104 pKa = 4.47LNEE107 pKa = 4.11TAVEE111 pKa = 3.9ALKK114 pKa = 11.18VLLEE118 pKa = 4.09ASASKK123 pKa = 10.65SEE125 pKa = 3.94KK126 pKa = 10.33SVNEE130 pKa = 4.2VIDD133 pKa = 4.26DD134 pKa = 3.93LSEE137 pKa = 3.96VAEE140 pKa = 3.89EE141 pKa = 4.2LGFNIGDD148 pKa = 3.99YY149 pKa = 10.49LPKK152 pKa = 10.59SMKK155 pKa = 10.23SSEE158 pKa = 4.22NLWEE162 pKa = 4.13ILEE165 pKa = 4.41SEE167 pKa = 4.34AGTIVSSVASFMDD180 pKa = 3.34NAEE183 pKa = 4.08LLKK186 pKa = 10.64FLTSGYY192 pKa = 9.55YY193 pKa = 10.86ALISTTDD200 pKa = 3.89DD201 pKa = 2.97ASMIYY206 pKa = 10.35AAFCAWYY213 pKa = 9.29DD214 pKa = 3.05ICYY217 pKa = 9.32MLNLVLDD224 pKa = 3.97IDD226 pKa = 4.17NDD228 pKa = 4.25GVITDD233 pKa = 3.97EE234 pKa = 5.15DD235 pKa = 4.19LVKK238 pKa = 10.37NTITSPASFTEE249 pKa = 4.49YY250 pKa = 11.06ASNTQSGLYY259 pKa = 9.72HH260 pKa = 7.39DD261 pKa = 5.49EE262 pKa = 4.22NSCDD266 pKa = 3.66EE267 pKa = 4.58FVWAYY272 pKa = 10.78DD273 pKa = 3.18ILTDD277 pKa = 4.75ILTTLDD283 pKa = 3.61IDD285 pKa = 4.34IDD287 pKa = 4.26LPDD290 pKa = 4.29APATQDD296 pKa = 4.48LYY298 pKa = 11.24DD299 pKa = 3.52AHH301 pKa = 6.7YY302 pKa = 10.94LPDD305 pKa = 4.09LFEE308 pKa = 5.02LLSGDD313 pKa = 4.2AEE315 pKa = 4.22

Molecular weight: 34.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8F5D6|A8F5D6_PSELT DegV family protein OS=Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO) OX=416591 GN=Tlet_0804 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.21QPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 10.07RR12 pKa = 11.84KK13 pKa = 7.27RR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84SRR23 pKa = 11.84TPSGRR28 pKa = 11.84RR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.45GRR39 pKa = 11.84WKK41 pKa = 10.38IAVV44 pKa = 3.39

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2040

0

2040

668507

38

1650

327.7

36.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.405 ± 0.047

0.95 ± 0.016

4.996 ± 0.039

7.019 ± 0.057

5.187 ± 0.04

6.479 ± 0.042

1.596 ± 0.021

8.644 ± 0.041

7.487 ± 0.041

9.938 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.022

4.301 ± 0.031

3.67 ± 0.03

2.861 ± 0.032

4.503 ± 0.041

6.489 ± 0.042

4.725 ± 0.031

7.529 ± 0.037

1.013 ± 0.02

3.668 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski