Bacillus phage Stills

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Slashvirus; Bacillus virus Stills

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3T6B4|A0A0E3T6B4_9CAUD Uncharacterized protein OS=Bacillus phage Stills OX=1610833 GN=CPT_Stills46 PE=4 SV=1
MM1 pKa = 7.9PFNANNTTQPPFQPGCMTLTNDD23 pKa = 4.38EE24 pKa = 4.46VIKK27 pKa = 11.12VQDD30 pKa = 3.12KK31 pKa = 10.5CAEE34 pKa = 4.05LEE36 pKa = 4.27ATFEE40 pKa = 4.68PNDD43 pKa = 3.84FQPVINDD50 pKa = 2.81NWIIARR56 pKa = 11.84YY57 pKa = 9.5NEE59 pKa = 4.28SNNYY63 pKa = 8.11TEE65 pKa = 5.14AVNGDD70 pKa = 3.92TYY72 pKa = 11.91NVIKK76 pKa = 10.64GYY78 pKa = 10.47NRR80 pKa = 11.84DD81 pKa = 3.33VNTQGIGQDD90 pKa = 3.37AEE92 pKa = 4.58GNGIIIPEE100 pKa = 4.2PEE102 pKa = 4.18QPTEE106 pKa = 4.14EE107 pKa = 4.33PTEE110 pKa = 3.9EE111 pKa = 4.4APVEE115 pKa = 4.11EE116 pKa = 4.68QPVEE120 pKa = 3.97QPTEE124 pKa = 3.99EE125 pKa = 4.25KK126 pKa = 10.82EE127 pKa = 4.14PTEE130 pKa = 4.22EE131 pKa = 4.01VV132 pKa = 3.22

Molecular weight: 14.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3T7M3|A0A0E3T7M3_9CAUD Uncharacterized protein OS=Bacillus phage Stills OX=1610833 GN=CPT_Stills47 PE=4 SV=1
MM1 pKa = 7.59ANHH4 pKa = 5.98YY5 pKa = 9.88KK6 pKa = 10.51NRR8 pKa = 11.84VRR10 pKa = 11.84RR11 pKa = 11.84NYY13 pKa = 10.0IGDD16 pKa = 3.38IKK18 pKa = 11.34VFMSDD23 pKa = 2.71HH24 pKa = 5.33AVIRR28 pKa = 11.84FVEE31 pKa = 4.21RR32 pKa = 11.84QNLEE36 pKa = 4.02VEE38 pKa = 4.5GLDD41 pKa = 3.17IDD43 pKa = 3.78ATRR46 pKa = 11.84TQIIRR51 pKa = 11.84KK52 pKa = 8.41FRR54 pKa = 11.84NTKK57 pKa = 9.71LAAFLPGSRR66 pKa = 11.84EE67 pKa = 3.32RR68 pKa = 11.84RR69 pKa = 11.84YY70 pKa = 10.16EE71 pKa = 3.78VAGSMNEE78 pKa = 3.57RR79 pKa = 11.84MQFICRR85 pKa = 11.84KK86 pKa = 6.49TASNKK91 pKa = 10.34YY92 pKa = 10.11IIISCQLQGKK102 pKa = 8.74RR103 pKa = 11.84DD104 pKa = 3.69NWWKK108 pKa = 11.06NEE110 pKa = 3.56GLVKK114 pKa = 10.67YY115 pKa = 10.38AA116 pKa = 4.45

Molecular weight: 13.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

24106

34

2243

219.1

24.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.5 ± 0.488

0.834 ± 0.126

6.459 ± 0.159

7.558 ± 0.39

4.601 ± 0.248

6.247 ± 0.281

1.672 ± 0.159

6.621 ± 0.177

8.633 ± 0.274

7.716 ± 0.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.937 ± 0.202

5.729 ± 0.211

2.701 ± 0.215

4.007 ± 0.324

3.916 ± 0.215

5.762 ± 0.246

6.335 ± 0.376

6.592 ± 0.174

1.116 ± 0.085

4.065 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski