Alicyclobacillus montanus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Alicyclobacillaceae; Alicyclobacillus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3064 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6RV26|A0A1M6RV26_9BACL Glyoxylase beta-lactamase superfamily II OS=Alicyclobacillus montanus OX=1830138 GN=SAMN05443507_112108 PE=4 SV=1
MM1 pKa = 7.25LRR3 pKa = 11.84SLGSAVSGMDD13 pKa = 4.29AFQTDD18 pKa = 4.03LDD20 pKa = 4.24VVGNNIANVNTIGYY34 pKa = 8.8KK35 pKa = 8.24SARR38 pKa = 11.84AVFADD43 pKa = 4.03MLSQTLSGATAPTYY57 pKa = 10.36TGATSTSTNLSGGVNAQQVGLGTQIEE83 pKa = 4.69AIQNEE88 pKa = 4.47WTQGADD94 pKa = 3.12QTTGNPLDD102 pKa = 3.72VAINGAGLFAVTPDD116 pKa = 4.0PNSSIALTNNQLSGSNSSVYY136 pKa = 7.51YY137 pKa = 10.05TRR139 pKa = 11.84AGDD142 pKa = 3.95FSLDD146 pKa = 3.51ANSNLVLPNGNVVLGYY162 pKa = 10.42EE163 pKa = 4.58VTSSSNSSPNTGNLVPININTLVTQAGGNPNSSSTTGSWTLASNPNVTIGSNGSITVTDD222 pKa = 3.75TSGNTHH228 pKa = 6.53VIGYY232 pKa = 8.51LALATFPNYY241 pKa = 10.56GGLQKK246 pKa = 10.47IGDD249 pKa = 4.47SEE251 pKa = 4.27WSVTGNSGQATFGQPSSTSGTLQSGAVEE279 pKa = 4.02QSNVDD284 pKa = 3.58LTQEE288 pKa = 3.87FAEE291 pKa = 4.83MIVAQNGYY299 pKa = 9.42AANSQVISTDD309 pKa = 3.13KK310 pKa = 11.12QILQTLMNNVV320 pKa = 3.18

Molecular weight: 32.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6MQH4|A0A1M6MQH4_9BACL 3-dehydroquinate synthase OS=Alicyclobacillus montanus OX=1830138 GN=SAMN05443507_104146 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 9.61PGNRR6 pKa = 11.84AAVQGANSSEE16 pKa = 3.93SRR18 pKa = 11.84VRR20 pKa = 11.84TRR22 pKa = 11.84KK23 pKa = 9.2IKK25 pKa = 9.98VRR27 pKa = 11.84SMYY30 pKa = 9.4QRR32 pKa = 11.84SRR34 pKa = 11.84FRR36 pKa = 11.84EE37 pKa = 3.89CFLIVMEE44 pKa = 4.35DD45 pKa = 3.54RR46 pKa = 11.84EE47 pKa = 4.33RR48 pKa = 11.84VVQKK52 pKa = 10.37LHH54 pKa = 6.34EE55 pKa = 4.28FSPYY59 pKa = 10.43CFFAGTSS66 pKa = 3.31

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3064

0

3064

883548

24

2711

288.4

32.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.343 ± 0.049

0.922 ± 0.014

4.513 ± 0.033

6.409 ± 0.046

4.068 ± 0.035

7.019 ± 0.045

2.649 ± 0.025

6.297 ± 0.041

4.386 ± 0.035

10.624 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.74 ± 0.019

3.527 ± 0.036

4.306 ± 0.027

4.703 ± 0.036

5.627 ± 0.045

6.503 ± 0.037

5.201 ± 0.036

7.458 ± 0.038

1.541 ± 0.021

3.165 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski