Klebsiella phage vB_KpnM_KpV52

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Jedunavirus; Klebsiella virus KpV80

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9SEV9|A0A1I9SEV9_9CAUD Putative tail fiber family protein OS=Klebsiella phage vB_KpnM_KpV52 OX=1912321 GN=kpv52_42 PE=4 SV=1
MM1 pKa = 7.92AEE3 pKa = 4.19LTSTGYY9 pKa = 9.8SVKK12 pKa = 10.61SQNDD16 pKa = 3.0WFDD19 pKa = 3.59EE20 pKa = 4.25EE21 pKa = 4.28KK22 pKa = 10.67QLYY25 pKa = 10.35LDD27 pKa = 5.34IDD29 pKa = 4.75SNWNLDD35 pKa = 3.15PSTPDD40 pKa = 3.47GLKK43 pKa = 9.09MAHH46 pKa = 6.79DD47 pKa = 4.33AEE49 pKa = 4.65IFSALDD55 pKa = 3.24EE56 pKa = 4.48VLQQAYY62 pKa = 10.17NSKK65 pKa = 10.83DD66 pKa = 3.36PNKK69 pKa = 10.71ASGYY73 pKa = 10.2DD74 pKa = 3.51LDD76 pKa = 4.94VICALTGTVRR86 pKa = 11.84SEE88 pKa = 4.03GTASTVTGFVLTGVAGTQVPAGTRR112 pKa = 11.84FEE114 pKa = 4.52SSVTGYY120 pKa = 10.93RR121 pKa = 11.84FTLDD125 pKa = 3.18QTWTLDD131 pKa = 3.37SSGTATVDD139 pKa = 3.23ITCTTVGEE147 pKa = 4.34IEE149 pKa = 4.58ADD151 pKa = 3.23ANTITTIVDD160 pKa = 3.73TVAGLVSVNNPTPATPGTSAEE181 pKa = 4.23SDD183 pKa = 3.05GSLRR187 pKa = 11.84LKK189 pKa = 10.49RR190 pKa = 11.84ATAVGLPGSNQVDD203 pKa = 3.73SMLGQLFNVDD213 pKa = 2.55GVRR216 pKa = 11.84RR217 pKa = 11.84VRR219 pKa = 11.84VYY221 pKa = 11.09EE222 pKa = 4.02NDD224 pKa = 3.25EE225 pKa = 4.03ATTDD229 pKa = 3.55SNGQPGHH236 pKa = 6.53SIAPIVDD243 pKa = 3.4GGTDD247 pKa = 4.0DD248 pKa = 4.34DD249 pKa = 4.11VAMAIYY255 pKa = 10.12LKK257 pKa = 10.4KK258 pKa = 10.36NPGVAFYY265 pKa = 10.53QAGTGVTVTVTSPTYY280 pKa = 8.95PTMTKK285 pKa = 9.97DD286 pKa = 3.14IKK288 pKa = 10.47FSRR291 pKa = 11.84PVYY294 pKa = 9.2VDD296 pKa = 2.87MVVVIEE302 pKa = 4.51IKK304 pKa = 10.65DD305 pKa = 3.9DD306 pKa = 3.66GTLPSQAILEE316 pKa = 4.09PLIRR320 pKa = 11.84DD321 pKa = 4.37AIMEE325 pKa = 4.23YY326 pKa = 10.67AAGGLIPTEE335 pKa = 4.23YY336 pKa = 10.73GFKK339 pKa = 10.12PDD341 pKa = 3.92GFDD344 pKa = 2.96IGEE347 pKa = 4.3TVPYY351 pKa = 10.09SSLYY355 pKa = 9.26TPINKK360 pKa = 9.51VIGSYY365 pKa = 10.9GNSYY369 pKa = 9.48VNSMTLNGGTANVTIKK385 pKa = 10.62FNEE388 pKa = 3.93LSRR391 pKa = 11.84WTTSNITVTIVV402 pKa = 2.6

Molecular weight: 42.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I9SEW7|A0A1I9SEW7_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnM_KpV52 OX=1912321 GN=kpv52_50 PE=4 SV=1
MM1 pKa = 7.47YY2 pKa = 7.54EE3 pKa = 4.07TKK5 pKa = 10.49SAAVKK10 pKa = 9.52PRR12 pKa = 11.84KK13 pKa = 10.14AGDD16 pKa = 3.21AGGACVKK23 pKa = 10.29PRR25 pKa = 11.84GKK27 pKa = 9.04TGDD30 pKa = 3.42AKK32 pKa = 10.82VKK34 pKa = 10.44RR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.68

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

14817

38

785

197.6

21.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.517 ± 0.407

1.485 ± 0.17

6.155 ± 0.198

5.77 ± 0.349

3.348 ± 0.213

7.525 ± 0.285

1.937 ± 0.185

5.777 ± 0.196

5.548 ± 0.282

7.296 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.158

4.988 ± 0.268

4.022 ± 0.211

3.921 ± 0.238

5.46 ± 0.238

6.061 ± 0.292

6.837 ± 0.285

7.458 ± 0.245

1.539 ± 0.095

3.55 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski