Gluconobacter phage GC1

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Kalamavirales; Tectiviridae; Gammatectivirus; Gluconobacter virus GC1

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I5AR68|A0A2I5AR68_9VIRU Uncharacterized protein OS=Gluconobacter phage GC1 OX=2047788 GN=GC1_00007 PE=4 SV=1
MM1 pKa = 7.68GFLLCALCAFTQEE14 pKa = 4.31GFNMALLSPAMQSKK28 pKa = 8.45STARR32 pKa = 11.84GKK34 pKa = 10.95VIDD37 pKa = 5.39QFVTWDD43 pKa = 3.3ATDD46 pKa = 3.17TDD48 pKa = 4.97GIVFDD53 pKa = 5.3FTAQGNAMLLRR64 pKa = 11.84EE65 pKa = 4.38PVCVFVDD72 pKa = 3.42NTRR75 pKa = 11.84ATGQLTITVEE85 pKa = 4.65GYY87 pKa = 10.53AYY89 pKa = 10.45QLVVQPGMIASLPFMSVALPRR110 pKa = 11.84VTVKK114 pKa = 10.66SSEE117 pKa = 4.19GTSGVTSFIFCDD129 pKa = 4.47FMLPNFAYY137 pKa = 8.77EE138 pKa = 4.03ATGISQKK145 pKa = 8.05TTDD148 pKa = 3.84TILEE152 pKa = 4.25EE153 pKa = 4.43VVSGGIGSEE162 pKa = 4.14SLRR165 pKa = 11.84VTDD168 pKa = 4.96AEE170 pKa = 4.71AIALLTTINSALSDD184 pKa = 3.53SGSTGNSSNATLKK197 pKa = 10.99AILAQLKK204 pKa = 7.44EE205 pKa = 4.33TLSVSDD211 pKa = 4.68ADD213 pKa = 4.39VLAQLKK219 pKa = 6.65TTLAVNDD226 pKa = 3.67GDD228 pKa = 5.89AIAEE232 pKa = 4.24LNVIEE237 pKa = 4.4ATLEE241 pKa = 3.9NSDD244 pKa = 3.5FTKK247 pKa = 10.66SVNVTGRR254 pKa = 11.84IQSSWIKK261 pKa = 10.56NVFNGSCLIDD271 pKa = 3.75KK272 pKa = 8.1LTVFPTDD279 pKa = 3.83PGSSVYY285 pKa = 10.5LAIFNEE291 pKa = 4.52TVPNSGQEE299 pKa = 4.03DD300 pKa = 3.97VTLKK304 pKa = 10.91VVGIYY309 pKa = 9.81PISKK313 pKa = 10.57GDD315 pKa = 3.5ILTLDD320 pKa = 4.06FSAPVFASTSFTIGFFASYY339 pKa = 8.57PIPGTGIAGSNSEE352 pKa = 4.62VYY354 pKa = 10.1DD355 pKa = 3.3VSYY358 pKa = 10.24IAQYY362 pKa = 10.79RR363 pKa = 11.84PVGNN367 pKa = 3.68

Molecular weight: 38.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I5AR73|A0A2I5AR73_9VIRU Uncharacterized protein OS=Gluconobacter phage GC1 OX=2047788 GN=GC1_00011 PE=4 SV=1
MM1 pKa = 7.96AIKK4 pKa = 9.98QWFIGLKK11 pKa = 9.99GKK13 pKa = 10.19RR14 pKa = 11.84LSLGAAQYY22 pKa = 11.21GFAGDD27 pKa = 3.69GALKK31 pKa = 10.08IVRR34 pKa = 11.84DD35 pKa = 4.06GNTVNNVQRR44 pKa = 11.84QIRR47 pKa = 11.84TMGAYY52 pKa = 7.95NTVPVLGQFVGVAQSSGSPKK72 pKa = 9.18TQGLQRR78 pKa = 11.84NPLTNDD84 pKa = 3.19LLTRR88 pKa = 11.84LYY90 pKa = 10.88GQGGQSS96 pKa = 2.94

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

5036

38

561

139.9

15.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.346 ± 0.771

0.913 ± 0.271

5.5 ± 0.403

5.56 ± 0.795

4.051 ± 0.404

7.824 ± 0.56

1.191 ± 0.198

6.116 ± 0.355

4.944 ± 0.827

7.605 ± 0.554

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.237 ± 0.3

4.567 ± 0.634

3.852 ± 0.338

3.376 ± 0.375

4.865 ± 0.621

7.347 ± 0.533

6.851 ± 0.725

7.327 ± 0.428

1.35 ± 0.177

3.177 ± 0.431
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski