Spirosoma sp. HMF3257

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Spirosoma; unclassified Spirosoma

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5857 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A327NHZ9|A0A327NHZ9_9BACT Type III pantothenate kinase OS=Spirosoma sp. HMF3257 OX=2183553 GN=coaX PE=3 SV=1
MM1 pKa = 7.71PAGLDD6 pKa = 3.59FDD8 pKa = 4.25PTTGVISGTPTNIGQFGITIGTSDD32 pKa = 3.72SQSTVYY38 pKa = 10.37RR39 pKa = 11.84GFYY42 pKa = 10.23LQINSSATVNLPPVVVSNLSSPITRR67 pKa = 11.84DD68 pKa = 2.6IYY70 pKa = 9.61QTISIPAAYY79 pKa = 10.19AFTDD83 pKa = 4.07PKK85 pKa = 11.16DD86 pKa = 3.68DD87 pKa = 3.9PP88 pKa = 4.69

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A327NKB0|A0A327NKB0_9BACT Phosphatidylglycerophosphatase A OS=Spirosoma sp. HMF3257 OX=2183553 GN=HMF3257_18140 PE=4 SV=1
MM1 pKa = 7.24NTPQTQQTKK10 pKa = 8.06RR11 pKa = 11.84TFRR14 pKa = 11.84VIALLGGLFLVIGVILRR31 pKa = 11.84LSGLLITATFAMALAGGVGLLLGLIGMATAQRR63 pKa = 11.84MKK65 pKa = 10.59PKK67 pKa = 10.54

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5857

0

5857

1723158

26

2328

294.2

32.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.943 ± 0.033

0.818 ± 0.011

5.202 ± 0.026

5.16 ± 0.034

4.505 ± 0.023

6.887 ± 0.033

1.865 ± 0.016

6.218 ± 0.025

5.346 ± 0.034

10.192 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.117 ± 0.014

4.733 ± 0.029

4.49 ± 0.02

4.519 ± 0.026

4.94 ± 0.026

6.526 ± 0.034

6.715 ± 0.042

6.665 ± 0.026

1.311 ± 0.013

3.845 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski