Methylovirgula sp. 4M-Z18

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Beijerinckiaceae; Methylovirgula; unclassified Methylovirgula

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4535 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371WU82|A0A371WU82_9RHIZ Exodeoxyribonuclease III OS=Methylovirgula sp. 4M-Z18 OX=2293567 GN=xth PE=3 SV=1
MM1 pKa = 7.25GVLMQINATFGAGSTTNFEE20 pKa = 4.48SAVNSAISFFDD31 pKa = 3.31KK32 pKa = 11.13AFTNNITLNINFNEE46 pKa = 4.0ISQAGGGLSSSSTEE60 pKa = 4.58LINDD64 pKa = 4.68GYY66 pKa = 11.0SAISTALKK74 pKa = 9.47TLNQTADD81 pKa = 3.64QISAAAALPASDD93 pKa = 4.65PFGSTSTASVSSAEE107 pKa = 4.0AKK109 pKa = 10.78ALGLSLNGYY118 pKa = 7.54TGADD122 pKa = 3.29GTVNLNDD129 pKa = 3.67AALPSGDD136 pKa = 2.97SWAWSQNSIGATQFDD151 pKa = 3.91AVGVIEE157 pKa = 4.68HH158 pKa = 7.21EE159 pKa = 4.08ISEE162 pKa = 4.37VMGRR166 pKa = 11.84IAGTTDD172 pKa = 2.54NGAQAGTLLDD182 pKa = 4.08TPEE185 pKa = 3.67AWHH188 pKa = 6.95IYY190 pKa = 8.67GASGSINTGTSASGTYY206 pKa = 10.37LSLNGGTTNLGAIGEE221 pKa = 4.51TGGGDD226 pKa = 3.35LADD229 pKa = 3.37WTDD232 pKa = 4.87PINATNDD239 pKa = 2.91AFGLIKK245 pKa = 10.55AGAVMQVTTTDD256 pKa = 3.36LRR258 pKa = 11.84EE259 pKa = 3.96MSTLGYY265 pKa = 10.48NLASEE270 pKa = 5.28ISQQNYY276 pKa = 8.95DD277 pKa = 3.42ASGKK281 pKa = 10.31LLGATVTVVDD291 pKa = 3.92SQQNVDD297 pKa = 3.31TVQIGFGATATTVTFSDD314 pKa = 3.5PAYY317 pKa = 9.11PNSPQTYY324 pKa = 8.76AAGAGALVLLSSGGTEE340 pKa = 4.1VNISSGSTTNEE351 pKa = 3.82VIISAGSDD359 pKa = 3.35SVTGGSGASLILSLGSGIYY378 pKa = 9.85RR379 pKa = 11.84AGSGTTQFALLSGNGEE395 pKa = 4.15VLASNSSISLGGGWNGFVEE414 pKa = 4.87GSGDD418 pKa = 3.8TITTLGGLGGGRR430 pKa = 11.84PDD432 pKa = 4.9VIGSSNNVTSTNVNDD447 pKa = 4.91FIEE450 pKa = 4.59VGDD453 pKa = 3.94AAGTTSANNVVHH465 pKa = 6.7FNAGGGTLALLDD477 pKa = 3.65NAQANVYY484 pKa = 9.94GDD486 pKa = 3.96HH487 pKa = 6.21LTVYY491 pKa = 10.35VDD493 pKa = 3.52NSSDD497 pKa = 3.5SLALQGSGDD506 pKa = 3.91NVTVDD511 pKa = 3.52TSNVSISVGGNGGAAGVATDD531 pKa = 3.93DD532 pKa = 3.98TVTFDD537 pKa = 5.03ASGTLNVSDD546 pKa = 4.36NSHH549 pKa = 5.22VDD551 pKa = 3.26AYY553 pKa = 10.53GSSVLVTAGTNDD565 pKa = 3.38TVGVQGSNDD574 pKa = 3.12AVHH577 pKa = 7.03ANGNNDD583 pKa = 2.72IVSIGGNGGTFNFSNNDD600 pKa = 3.05GVTVAGTGTTVNEE613 pKa = 4.48LDD615 pKa = 3.74NSHH618 pKa = 6.11VDD620 pKa = 3.15AYY622 pKa = 11.01GNNFAANAGTGDD634 pKa = 3.55TLGVHH639 pKa = 6.77GGGITVNASGNNDD652 pKa = 3.74TVWAGGNSSTFSFADD667 pKa = 3.66DD668 pKa = 3.82DD669 pKa = 4.5TVTFAGTGGTLNEE682 pKa = 4.62SDD684 pKa = 4.14NSHH687 pKa = 6.2VDD689 pKa = 3.26ATGSNFWVNAGTGDD703 pKa = 3.57TVGVHH708 pKa = 6.2GGGITVNASGNNDD721 pKa = 3.74TVWAGGNGQAFSFATDD737 pKa = 3.79DD738 pKa = 4.08SIHH741 pKa = 6.79FYY743 pKa = 9.92GTGSTLNEE751 pKa = 4.3FDD753 pKa = 4.0NSHH756 pKa = 6.56IDD758 pKa = 3.67GFGSNLWVNAGTNDD772 pKa = 3.52TLGVQGSGITANANGNTDD790 pKa = 3.89TVWASGNGQAFSFATDD806 pKa = 3.37DD807 pKa = 3.52SIYY810 pKa = 10.67FHH812 pKa = 6.61GTGGTLNEE820 pKa = 4.49SDD822 pKa = 4.24SSHH825 pKa = 7.17VDD827 pKa = 3.03ASGNGVWVNAGSNDD841 pKa = 3.38TLGLHH846 pKa = 6.91GSAITANASGSNDD859 pKa = 3.79TVWAGGNGQAFSFTTDD875 pKa = 2.97DD876 pKa = 4.3SINLGGSGGVVNEE889 pKa = 4.55FDD891 pKa = 3.79NSHH894 pKa = 6.88VDD896 pKa = 2.91ISGNGLTANVGNNDD910 pKa = 2.94TFGAHH915 pKa = 6.28GSSDD919 pKa = 3.58VVHH922 pKa = 7.06ANGNSDD928 pKa = 3.94TVWLGGNGQNASAHH942 pKa = 6.01DD943 pKa = 3.78AVYY946 pKa = 9.59FAGSAGVLNLADD958 pKa = 4.12NSTVDD963 pKa = 3.29ATGNNVWANLGNNDD977 pKa = 3.77TLNLFGSGDD986 pKa = 3.74GFSASGSGDD995 pKa = 3.78VVWLNGVRR1003 pKa = 11.84VRR1005 pKa = 4.8

Molecular weight: 99.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371WPG6|A0A371WPG6_9RHIZ Arylesterase OS=Methylovirgula sp. 4M-Z18 OX=2293567 GN=DYH55_14740 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4535

0

4535

1443161

28

5431

318.2

34.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.56 ± 0.049

0.906 ± 0.013

5.631 ± 0.031

5.142 ± 0.04

3.836 ± 0.027

8.436 ± 0.098

2.27 ± 0.018

5.416 ± 0.026

3.569 ± 0.035

10.121 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.43 ± 0.018

2.802 ± 0.033

5.138 ± 0.031

3.346 ± 0.022

6.609 ± 0.054

5.464 ± 0.048

5.346 ± 0.035

7.331 ± 0.026

1.34 ± 0.014

2.31 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski