Peribacillus asahii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Peribacillus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4015 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A398AW79|A0A398AW79_9BACI Aspartyl-phosphate phosphatase Spo0E family protein OS=Peribacillus asahii OX=228899 GN=D1953_19850 PE=4 SV=1
MM1 pKa = 7.37FNIIQTKK8 pKa = 10.23KK9 pKa = 10.16YY10 pKa = 10.24DD11 pKa = 3.28GFYY14 pKa = 10.85NIIFTYY20 pKa = 9.84EE21 pKa = 3.75DD22 pKa = 4.42EE23 pKa = 4.57YY24 pKa = 11.58FLAAGEE30 pKa = 4.36YY31 pKa = 10.75EE32 pKa = 4.01NDD34 pKa = 3.12QTQLVILDD42 pKa = 5.07AIDD45 pKa = 3.76KK46 pKa = 10.89KK47 pKa = 10.95DD48 pKa = 3.31IKK50 pKa = 11.25EE51 pKa = 4.08NMLKK55 pKa = 9.33TDD57 pKa = 3.87YY58 pKa = 10.61ISYY61 pKa = 10.66YY62 pKa = 10.71SLIDD66 pKa = 3.64DD67 pKa = 4.54VEE69 pKa = 4.35NHH71 pKa = 7.1DD72 pKa = 3.62VLQYY76 pKa = 10.37IDD78 pKa = 5.1EE79 pKa = 4.41ILSTGLQLDD88 pKa = 4.04TEE90 pKa = 4.62LASS93 pKa = 3.58

Molecular weight: 10.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A398AZD7|A0A398AZD7_9BACI Acyl-CoA thioesterase OS=Peribacillus asahii OX=228899 GN=D1953_17120 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.91VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4015

0

4015

1174495

17

1579

292.5

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.245 ± 0.038

0.749 ± 0.012

4.768 ± 0.031

7.543 ± 0.042

4.477 ± 0.035

6.787 ± 0.04

2.083 ± 0.02

7.924 ± 0.041

6.91 ± 0.034

9.812 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.713 ± 0.018

4.267 ± 0.024

3.565 ± 0.022

4.006 ± 0.026

4.022 ± 0.028

5.972 ± 0.033

5.521 ± 0.026

7.12 ± 0.032

1.005 ± 0.014

3.511 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski