Faba bean polerovirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A455KZJ8|A0A455KZJ8_9LUTE Readthrough protein OS=Faba bean polerovirus 1 OX=2283388 PE=3 SV=1
MM1 pKa = 7.31SLDD4 pKa = 3.23VVTIDD9 pKa = 3.96RR10 pKa = 11.84RR11 pKa = 11.84TSQVLFNDD19 pKa = 3.43SLFNYY24 pKa = 10.31SLFLRR29 pKa = 11.84RR30 pKa = 11.84SILIATIQSFNYY42 pKa = 10.01LCEE45 pKa = 4.12TSSLIHH51 pKa = 5.91GTNKK55 pKa = 9.95AISMLSFLFQLPFIYY70 pKa = 10.25GGFEE74 pKa = 3.92LRR76 pKa = 11.84GGALVLPHH84 pKa = 6.11SRR86 pKa = 11.84KK87 pKa = 8.83SKK89 pKa = 10.1KK90 pKa = 9.64RR91 pKa = 11.84RR92 pKa = 11.84TFNKK96 pKa = 10.05LLADD100 pKa = 3.8NGLYY104 pKa = 10.36ALKK107 pKa = 10.56LGDD110 pKa = 4.15RR111 pKa = 11.84GTPTSTNIQITAGGSYY127 pKa = 10.24LYY129 pKa = 10.51KK130 pKa = 10.74YY131 pKa = 9.08IQNPLAEE138 pKa = 5.05NIRR141 pKa = 11.84GHH143 pKa = 6.81ADD145 pKa = 2.94VFEE148 pKa = 4.38FGKK151 pKa = 9.92NHH153 pKa = 7.13FKK155 pKa = 10.93RR156 pKa = 11.84LLNAWCLHH164 pKa = 5.47LSRR167 pKa = 11.84RR168 pKa = 11.84NFEE171 pKa = 4.49LYY173 pKa = 10.05PVFSLGDD180 pKa = 3.69NLAMVLDD187 pKa = 4.17SLADD191 pKa = 3.66NLVGFAGINQQYY203 pKa = 8.88HH204 pKa = 6.05ASGFLRR210 pKa = 11.84IARR213 pKa = 11.84DD214 pKa = 3.21LHH216 pKa = 6.03GLYY219 pKa = 10.91GEE221 pKa = 4.61TAASTVWQLFGLDD234 pKa = 3.21RR235 pKa = 11.84CPCVEE240 pKa = 4.72DD241 pKa = 3.98TPFPNIILLQEE252 pKa = 4.48RR253 pKa = 11.84IEE255 pKa = 4.26SEE257 pKa = 3.92EE258 pKa = 4.61GEE260 pKa = 4.32DD261 pKa = 5.7GEE263 pKa = 5.69GFWEE267 pKa = 4.27LL268 pKa = 3.81

Molecular weight: 30.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A455KZJ6|A0A455KZJ6_9LUTE P0 protein OS=Faba bean polerovirus 1 OX=2283388 PE=4 SV=1
MM1 pKa = 6.49NTVVGRR7 pKa = 11.84RR8 pKa = 11.84TINGRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84PRR17 pKa = 11.84RR18 pKa = 11.84QTRR21 pKa = 11.84RR22 pKa = 11.84AQRR25 pKa = 11.84SQPVVVVQTSRR36 pKa = 11.84TTQRR40 pKa = 11.84RR41 pKa = 11.84PRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84GNNRR51 pKa = 11.84TGGAVSTRR59 pKa = 11.84GASSSEE65 pKa = 3.81TFVFSKK71 pKa = 11.13DD72 pKa = 3.3NLAGSSSGAITFGPSLSDD90 pKa = 4.1CPAFSNGILKK100 pKa = 10.26AYY102 pKa = 10.04HH103 pKa = 6.58EE104 pKa = 4.78YY105 pKa = 10.01KK106 pKa = 10.07ISMVILEE113 pKa = 4.63FVSEE117 pKa = 4.2ASSQNSGSIAYY128 pKa = 9.39EE129 pKa = 3.85LDD131 pKa = 3.29PHH133 pKa = 6.98CKK135 pKa = 10.11LNSLSSTINKK145 pKa = 9.78FGITKK150 pKa = 9.57PGRR153 pKa = 11.84RR154 pKa = 11.84TFTASYY160 pKa = 10.57INGTEE165 pKa = 3.71WHH167 pKa = 7.15DD168 pKa = 3.61VAEE171 pKa = 4.53DD172 pKa = 3.57QFKK175 pKa = 10.61ILYY178 pKa = 8.79KK179 pKa = 10.96GNGSSSIAGSFRR191 pKa = 11.84ITIKK195 pKa = 10.85CQFHH199 pKa = 6.04NPKK202 pKa = 10.51

Molecular weight: 22.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

1

6

2954

175

1033

492.3

55.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.161 ± 0.129

1.354 ± 0.197

3.995 ± 0.497

5.924 ± 0.309

4.13 ± 0.476

7.38 ± 0.215

1.93 ± 0.235

5.213 ± 0.376

5.924 ± 0.578

9.174 ± 1.106

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.309

4.942 ± 0.297

5.247 ± 0.456

4.333 ± 0.314

6.127 ± 0.701

9.885 ± 0.617

5.89 ± 0.566

5.281 ± 0.47

1.997 ± 0.313

2.776 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski