Bulleidia extructa W1219

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Bulleidia; Bulleidia extructa

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1415 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2MN66|D2MN66_9FIRM Hydrolase alpha/beta domain protein OS=Bulleidia extructa W1219 OX=679192 GN=HMPREF9013_0083 PE=4 SV=1
MM1 pKa = 7.58AKK3 pKa = 10.22LAVVYY8 pKa = 9.17YY9 pKa = 10.21SATGNCQMMAEE20 pKa = 4.18AVVAGGQSAGGEE32 pKa = 4.27VEE34 pKa = 5.17LIEE37 pKa = 6.18AGDD40 pKa = 3.89FTAGDD45 pKa = 3.61VANYY49 pKa = 7.85DD50 pKa = 4.08TIAFGCSAYY59 pKa = 10.28GAEE62 pKa = 4.19NLEE65 pKa = 4.36EE66 pKa = 4.49GTFEE70 pKa = 4.73PMFQEE75 pKa = 4.96VEE77 pKa = 4.17GQLSGKK83 pKa = 8.82TIGLFGSYY91 pKa = 10.41DD92 pKa = 3.28WGDD95 pKa = 3.77GSWMRR100 pKa = 11.84EE101 pKa = 3.61WQDD104 pKa = 3.09RR105 pKa = 11.84VVADD109 pKa = 4.15GANLIQEE116 pKa = 4.33GVIANLTPDD125 pKa = 3.63EE126 pKa = 5.01EE127 pKa = 4.39GLEE130 pKa = 4.24ACRR133 pKa = 11.84QLGAALVKK141 pKa = 10.76

Molecular weight: 14.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2MMY7|D2MMY7_9FIRM Uncharacterized protein OS=Bulleidia extructa W1219 OX=679192 GN=HMPREF9013_1123 PE=4 SV=1
MM1 pKa = 7.52MKK3 pKa = 9.42RR4 pKa = 11.84TYY6 pKa = 10.26QPSKK10 pKa = 10.11RR11 pKa = 11.84KK12 pKa = 8.0TKK14 pKa = 8.82ATHH17 pKa = 5.88GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTVGGRR29 pKa = 11.84KK30 pKa = 8.73VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.94GRR40 pKa = 11.84KK41 pKa = 8.67VLSAA45 pKa = 4.05

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1415

0

1415

428656

37

2908

302.9

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.245 ± 0.068

1.062 ± 0.026

5.225 ± 0.053

7.369 ± 0.074

4.463 ± 0.064

6.356 ± 0.067

2.234 ± 0.033

7.604 ± 0.07

8.148 ± 0.075

9.753 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.9 ± 0.038

4.521 ± 0.05

3.037 ± 0.035

4.112 ± 0.055

4.083 ± 0.037

6.146 ± 0.05

4.866 ± 0.058

6.808 ± 0.064

0.972 ± 0.023

4.095 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski