Sulfolobus islandicus filamentous virus (isolate Iceland/Hveragerdi) (SIFV)

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Lipothrixviridae; Betalipothrixvirus; Sulfolobus islandicus filamentous virus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q914H7|Y055_SIFVH Uncharacterized protein 55 OS=Sulfolobus islandicus filamentous virus (isolate Iceland/Hveragerdi) OX=654908 GN=SIFV0055 PE=4 SV=1
MM1 pKa = 7.77SFLLNLGQAFFSGLSSIATGVTSDD25 pKa = 3.51VTSVANGINYY35 pKa = 9.13AFNVLANFLQKK46 pKa = 10.62LPSEE50 pKa = 4.11IATFFQSIPGALIGFAHH67 pKa = 6.79TFGTYY72 pKa = 8.77IWDD75 pKa = 3.55GMQRR79 pKa = 11.84IASGFTAIMAPIEE92 pKa = 4.5RR93 pKa = 11.84GLEE96 pKa = 4.05TLGTTVINALTVVWNDD112 pKa = 2.43IKK114 pKa = 11.33AFASAIYY121 pKa = 10.3NSIVSMINNIVSFVQPFINDD141 pKa = 3.83FKK143 pKa = 11.04NAISFAYY150 pKa = 10.29NFLLNVINDD159 pKa = 3.49VYY161 pKa = 11.42SAFTIISNFFLDD173 pKa = 5.26LPTFFQNASNYY184 pKa = 10.02LNSLFTDD191 pKa = 4.43PGNQPNLLTMVPNLVASEE209 pKa = 4.07VSRR212 pKa = 11.84IAGAFPDD219 pKa = 4.22VIAYY223 pKa = 6.7NTFMEE228 pKa = 4.74VLPKK232 pKa = 9.45MVSGIASSPIFGNTVKK248 pKa = 10.84GMFAKK253 pKa = 10.37ALLMAGSPILSALLSEE269 pKa = 4.33LTKK272 pKa = 11.29ALMQSLFTSTQTTQTTQRR290 pKa = 11.84PSQPQIQPPTAPSTQLQQRR309 pKa = 11.84STQQTSLSDD318 pKa = 3.65LQNLQTQQLTPTQIEE333 pKa = 4.49VQLEE337 pKa = 4.24RR338 pKa = 11.84PNVTGVFTQDD348 pKa = 3.36VIGMGTASGGTAKK361 pKa = 10.79LVTGYY366 pKa = 10.62INFQNSIKK374 pKa = 10.46QFEE377 pKa = 4.59DD378 pKa = 3.39VFNITASFFMEE389 pKa = 5.74LIQSLQTEE397 pKa = 5.28FSQDD401 pKa = 2.89MAVNVSLTLLEE412 pKa = 4.75NIYY415 pKa = 10.34QVSQSIEE422 pKa = 3.96IIPSVDD428 pKa = 2.73ATTIILPPGISLCNPSQAPSPSEE451 pKa = 4.02STSTSTNSIDD461 pKa = 3.18VTTTVDD467 pKa = 3.42VTEE470 pKa = 4.49EE471 pKa = 4.14LCIPPYY477 pKa = 10.83DD478 pKa = 4.18LLADD482 pKa = 4.18GLTTQFAVIALLHH495 pKa = 6.18SNISDD500 pKa = 3.32VMLAITQLIYY510 pKa = 10.82GITFSNLISDD520 pKa = 4.29ILSATTQLMYY530 pKa = 10.6SITSNISTSDD540 pKa = 3.43TVSVSASFTPSPPPSEE556 pKa = 4.12TYY558 pKa = 9.46TYY560 pKa = 10.55YY561 pKa = 11.02VPIDD565 pKa = 3.28IAVTYY570 pKa = 7.37TIQVASSTTYY580 pKa = 10.68SGALSYY586 pKa = 10.07TVSTSS591 pKa = 2.73

Molecular weight: 63.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q914I4|GT048_SIFVH Putative glycosyltransferase 48 OS=Sulfolobus islandicus filamentous virus (isolate Iceland/Hveragerdi) OX=654908 GN=SIFV0048 PE=3 SV=1
MM1 pKa = 7.04VRR3 pKa = 11.84ALLFHH8 pKa = 6.84VITYY12 pKa = 7.58TKK14 pKa = 10.39FIVPVVKK21 pKa = 10.35LLIMSAIAGVIAGAFGGGIGGVGDD45 pKa = 5.17AIGTIIGDD53 pKa = 3.99LEE55 pKa = 3.76RR56 pKa = 11.84AIARR60 pKa = 11.84FGGSIVNAFKK70 pKa = 10.4TVIDD74 pKa = 4.82KK75 pKa = 10.83ILTLAVRR82 pKa = 11.84IGRR85 pKa = 11.84IIEE88 pKa = 4.01KK89 pKa = 8.88YY90 pKa = 9.9FRR92 pKa = 11.84IAVHH96 pKa = 6.0YY97 pKa = 9.95IVLFLRR103 pKa = 11.84LAYY106 pKa = 10.06RR107 pKa = 11.84YY108 pKa = 7.88MYY110 pKa = 10.8SFYY113 pKa = 10.92TEE115 pKa = 3.97FQKK118 pKa = 11.18DD119 pKa = 3.44PWRR122 pKa = 11.84SLQFVGSMAILLNNSLFPP140 pKa = 4.81

Molecular weight: 15.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

12208

31

601

167.2

19.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.595 ± 0.299

1.204 ± 0.168

4.481 ± 0.242

6.873 ± 0.471

4.546 ± 0.216

4.718 ± 0.193

1.466 ± 0.144

9.469 ± 0.349

7.831 ± 0.597

9.092 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.114

6.176 ± 0.294

3.408 ± 0.222

3.268 ± 0.304

3.53 ± 0.288

6.758 ± 0.49

6.184 ± 0.503

7.298 ± 0.341

0.836 ± 0.106

5.734 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski