Gordonia phage GordDuk1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gordtnkvirus; unclassified Gordtnkvirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0E3T6W6|A0A0E3T6W6_9CAUD Uncharacterized protein OS=Gordonia phage GordDuk1 OX=1622191 GN=GordDuk1_81 PE=4 SV=1
MM1 pKa = 8.3AEE3 pKa = 4.05MYY5 pKa = 8.2EE6 pKa = 4.28PPGSRR11 pKa = 11.84EE12 pKa = 4.03LMDD15 pKa = 4.16EE16 pKa = 4.74LSLAEE21 pKa = 4.39QNPIAQGDD29 pKa = 3.97GWSLLGPFAADD40 pKa = 3.31GTYY43 pKa = 10.78LPDD46 pKa = 4.21LEE48 pKa = 6.21AGVRR52 pKa = 11.84YY53 pKa = 10.13DD54 pKa = 3.38SASNTVIIPP63 pKa = 3.71

Molecular weight:
6.77 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0E3T645|A0A0E3T645_9CAUD DNA binding domain protein OS=Gordonia phage GordDuk1 OX=1622191 GN=GordDuk1_58 PE=4 SV=1
MM1 pKa = 7.2QNQDD5 pKa = 3.27MRR7 pKa = 11.84HH8 pKa = 5.16SSEE11 pKa = 3.84MAKK14 pKa = 10.5YY15 pKa = 9.78LAIASGLTGKK25 pKa = 10.09RR26 pKa = 11.84QEE28 pKa = 3.91LQAAKK33 pKa = 10.09KK34 pKa = 10.52AKK36 pKa = 10.4DD37 pKa = 3.22KK38 pKa = 11.1AKK40 pKa = 10.43RR41 pKa = 11.84RR42 pKa = 11.84AKK44 pKa = 10.25RR45 pKa = 11.84KK46 pKa = 7.73RR47 pKa = 11.84TGRR50 pKa = 3.49

Molecular weight:
5.73 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

23139

40

3272

238.5

26.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.058 ± 0.799

0.985 ± 0.162

6.513 ± 0.459

6.422 ± 0.335

3.95 ± 0.13

7.662 ± 0.436

1.673 ± 0.173

5.804 ± 0.187

5.683 ± 0.253

7.36 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.818 ± 0.134

5.022 ± 0.18

4.819 ± 0.332

3.799 ± 0.216

5.087 ± 0.23

6.673 ± 0.276

5.744 ± 0.228

6.5 ± 0.222

1.5 ± 0.115

2.93 ± 0.183

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski