Otarine picobirnavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Picobirnaviridae; Picobirnavirus; unclassified Picobirnavirus

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2DA78|I2DA78_9VIRU Putative RNA-dependent RNA polymerase OS=Otarine picobirnavirus OX=1187973 PE=4 SV=1
MM1 pKa = 7.54SKK3 pKa = 9.84MSTKK7 pKa = 10.5DD8 pKa = 3.29RR9 pKa = 11.84NNDD12 pKa = 3.35SLNGGTNNNARR23 pKa = 11.84QKK25 pKa = 10.97SNNKK29 pKa = 9.14RR30 pKa = 11.84RR31 pKa = 11.84GSKK34 pKa = 10.16NPNRR38 pKa = 11.84PSTRR42 pKa = 11.84NHH44 pKa = 5.86VGEE47 pKa = 4.43DD48 pKa = 3.23QHH50 pKa = 6.12YY51 pKa = 8.2TLANAPDD58 pKa = 4.29AGNSIDD64 pKa = 4.29WYY66 pKa = 10.21NKK68 pKa = 10.39SPVLLKK74 pKa = 10.72DD75 pKa = 3.23AANITMAHH83 pKa = 6.01PVGLGMSLVMPQGTSGKK100 pKa = 9.89VGTYY104 pKa = 9.93SDD106 pKa = 4.17PGIMALNFIPTIASGSSGTHH126 pKa = 5.8ASDD129 pKa = 3.61INSATNIAARR139 pKa = 11.84NIYY142 pKa = 10.55SFVRR146 pKa = 11.84HH147 pKa = 5.79TNSGHH152 pKa = 6.46ANYY155 pKa = 9.02DD156 pKa = 3.31APDD159 pKa = 3.43LMKK162 pKa = 10.89YY163 pKa = 10.1LIAMDD168 pKa = 4.91SIYY171 pKa = 10.3MVYY174 pKa = 10.31AHH176 pKa = 7.41CARR179 pKa = 11.84IYY181 pKa = 10.83GVLRR185 pKa = 11.84LYY187 pKa = 10.74DD188 pKa = 3.5GRR190 pKa = 11.84NRR192 pKa = 11.84YY193 pKa = 9.27YY194 pKa = 9.99PDD196 pKa = 3.39QILRR200 pKa = 11.84ALGVANVTPQMGINEE215 pKa = 4.09FRR217 pKa = 11.84SALNRR222 pKa = 11.84IAYY225 pKa = 9.04KK226 pKa = 10.05IGVMNVPNNLSYY238 pKa = 9.57MQRR241 pKa = 11.84HH242 pKa = 5.44MWLCSNLYY250 pKa = 10.47ADD252 pKa = 4.25EE253 pKa = 5.23PGNTFQTYY261 pKa = 9.2CFRR264 pKa = 11.84PGGAYY269 pKa = 9.53RR270 pKa = 11.84YY271 pKa = 9.76RR272 pKa = 11.84FSTPKK277 pKa = 10.7DD278 pKa = 3.39PTGSLEE284 pKa = 4.23MKK286 pKa = 10.12VLPTMSLQSWLNTLEE301 pKa = 4.18EE302 pKa = 4.11LMAPVLSSEE311 pKa = 4.73DD312 pKa = 3.59MAIMSGDD319 pKa = 3.58LLKK322 pKa = 10.78AYY324 pKa = 10.19GEE326 pKa = 4.12SSMFKK331 pKa = 9.7LTTVPEE337 pKa = 4.74DD338 pKa = 3.68YY339 pKa = 10.58QVLPTYY345 pKa = 10.56NEE347 pKa = 3.98EE348 pKa = 3.96VLSQIEE354 pKa = 4.44NATIWYY360 pKa = 9.4GIDD363 pKa = 3.44NSVEE367 pKa = 4.12SADD370 pKa = 4.02KK371 pKa = 10.87DD372 pKa = 3.42HH373 pKa = 6.99HH374 pKa = 6.37KK375 pKa = 10.5WDD377 pKa = 3.22IRR379 pKa = 11.84EE380 pKa = 4.01NTTQDD385 pKa = 3.63DD386 pKa = 4.33ANLGALYY393 pKa = 10.18QNPTLLSPYY402 pKa = 9.44GCQFDD407 pKa = 4.71SVINLHH413 pKa = 5.38TEE415 pKa = 3.79EE416 pKa = 4.39VNPEE420 pKa = 3.63NVMIATRR427 pKa = 11.84LMTTAVSAKK436 pKa = 8.28WMKK439 pKa = 10.0TKK441 pKa = 10.57EE442 pKa = 4.04KK443 pKa = 9.8LYY445 pKa = 10.77TMTAMDD451 pKa = 4.43YY452 pKa = 10.46GSEE455 pKa = 4.11IIVAAFMYY463 pKa = 9.84TGLGSANIDD472 pKa = 3.89DD473 pKa = 4.69LNCYY477 pKa = 10.18VDD479 pKa = 4.09IFSDD483 pKa = 3.67TTPAQAVSEE492 pKa = 4.47TAVSMIAAGYY502 pKa = 8.2LQTFNRR508 pKa = 11.84HH509 pKa = 5.14PLVFVGTRR517 pKa = 11.84TFKK520 pKa = 11.12DD521 pKa = 3.36PASTTTPSEE530 pKa = 3.83PTIYY534 pKa = 10.26TPAFMLGVSNMSTVDD549 pKa = 3.19HH550 pKa = 6.44RR551 pKa = 11.84TLEE554 pKa = 4.26SMHH557 pKa = 5.97NVAMLSMFDD566 pKa = 3.49VSSNNLNRR574 pKa = 11.84GG575 pKa = 3.25

Molecular weight: 63.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2DA77|I2DA77_9VIRU Capsid protein OS=Otarine picobirnavirus OX=1187973 GN=ORF2 PE=4 SV=1
MM1 pKa = 6.88TTNQINFFRR10 pKa = 11.84TQNEE14 pKa = 3.57KK15 pKa = 10.6LAAEE19 pKa = 4.85RR20 pKa = 11.84SAQQHH25 pKa = 4.52EE26 pKa = 4.54RR27 pKa = 11.84EE28 pKa = 4.25LNEE31 pKa = 3.48IRR33 pKa = 11.84RR34 pKa = 11.84SNLAKK39 pKa = 8.67EE40 pKa = 4.13QEE42 pKa = 4.08MRR44 pKa = 11.84RR45 pKa = 11.84SNMAKK50 pKa = 10.16EE51 pKa = 4.02NLGFQQFNEE60 pKa = 4.28SLRR63 pKa = 11.84HH64 pKa = 4.99NKK66 pKa = 8.16ATEE69 pKa = 4.15SQASSQLRR77 pKa = 11.84EE78 pKa = 3.97IEE80 pKa = 4.01RR81 pKa = 11.84HH82 pKa = 4.69NRR84 pKa = 11.84ASVGLGYY91 pKa = 11.05SNLSEE96 pKa = 3.96QHH98 pKa = 5.23RR99 pKa = 11.84HH100 pKa = 4.47NVASNSLGYY109 pKa = 10.99ANQFEE114 pKa = 4.7QNRR117 pKa = 11.84HH118 pKa = 5.38DD119 pKa = 3.69VAQEE123 pKa = 4.07LEE125 pKa = 4.42SQRR128 pKa = 11.84HH129 pKa = 4.15NTTTEE134 pKa = 3.66QTSNFGTIGNVLLGVGGLFTKK155 pKa = 10.46SNKK158 pKa = 9.05KK159 pKa = 10.05LKK161 pKa = 10.41RR162 pKa = 3.6

Molecular weight: 18.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1269

162

575

423.0

47.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.698 ± 0.836

1.182 ± 0.407

6.225 ± 1.567

4.886 ± 1.437

3.31 ± 0.455

6.147 ± 0.168

2.522 ± 0.456

4.413 ± 0.513

5.122 ± 0.95

7.723 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.255 ± 0.823

6.698 ± 1.879

4.571 ± 1.146

4.728 ± 1.542

5.674 ± 1.081

7.565 ± 0.937

6.619 ± 0.856

5.91 ± 0.819

1.34 ± 0.437

4.413 ± 0.85
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski