Isfahan virus (ISFV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Vesiculovirus; Isfahan vesiculovirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q5K2K7|NCAP_ISFV Nucleoprotein OS=Isfahan virus OX=290008 GN=N PE=3 SV=1
MM1 pKa = 7.54SRR3 pKa = 11.84LNQILKK9 pKa = 10.27DD10 pKa = 4.0YY11 pKa = 9.9PLLEE15 pKa = 4.4ATTSEE20 pKa = 4.23IEE22 pKa = 4.27SMEE25 pKa = 4.18SSLADD30 pKa = 4.4DD31 pKa = 5.06VITSNDD37 pKa = 3.57DD38 pKa = 3.74EE39 pKa = 4.52IQSVSPQYY47 pKa = 10.84YY48 pKa = 9.87LRR50 pKa = 11.84DD51 pKa = 3.51MFKK54 pKa = 11.17ASITEE59 pKa = 4.2GPDD62 pKa = 3.27DD63 pKa = 5.26DD64 pKa = 4.97FPPVPEE70 pKa = 4.25VEE72 pKa = 3.95NDD74 pKa = 3.26IILDD78 pKa = 3.92DD79 pKa = 4.3DD80 pKa = 4.27EE81 pKa = 6.36EE82 pKa = 5.8YY83 pKa = 11.06DD84 pKa = 3.77GYY86 pKa = 11.53KK87 pKa = 10.27VDD89 pKa = 4.23FAEE92 pKa = 4.25ARR94 pKa = 11.84PWTALTQKK102 pKa = 10.84NIDD105 pKa = 2.88GRR107 pKa = 11.84MNLEE111 pKa = 3.91LMAPEE116 pKa = 4.72NLTDD120 pKa = 3.51AQYY123 pKa = 9.99KK124 pKa = 8.64QWVEE128 pKa = 4.28SVSSIMTISRR138 pKa = 11.84QIRR141 pKa = 11.84LHH143 pKa = 5.01QAEE146 pKa = 4.42IMDD149 pKa = 4.16TSSGLLIIEE158 pKa = 4.25NMIPSIGRR166 pKa = 11.84TSEE169 pKa = 3.93FKK171 pKa = 10.7SIPEE175 pKa = 4.83HH176 pKa = 6.47IPPSPTSDD184 pKa = 2.72HH185 pKa = 5.67TTPPSSLRR193 pKa = 11.84SDD195 pKa = 3.78TPSQTSSSSMGLPDD209 pKa = 4.12VSSASDD215 pKa = 3.2WSGMINKK222 pKa = 9.71KK223 pKa = 9.79IRR225 pKa = 11.84IPPVVSSKK233 pKa = 10.99SPYY236 pKa = 10.57EE237 pKa = 3.88FTLSDD242 pKa = 4.25LYY244 pKa = 11.46GSNQAALDD252 pKa = 3.95YY253 pKa = 11.11LSGSGMDD260 pKa = 4.76LRR262 pKa = 11.84TAVCSGLKK270 pKa = 9.48QRR272 pKa = 11.84GIYY275 pKa = 9.78NRR277 pKa = 11.84IRR279 pKa = 11.84IQYY282 pKa = 9.2KK283 pKa = 8.03ITPEE287 pKa = 4.03FVV289 pKa = 2.79

Molecular weight: 32.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q5K2K6|PHOSP_ISFV Phosphoprotein OS=Isfahan virus OX=290008 GN=P PE=3 SV=1
MM1 pKa = 7.65KK2 pKa = 10.29SLKK5 pKa = 10.36RR6 pKa = 11.84LIKK9 pKa = 10.25SNKK12 pKa = 9.18KK13 pKa = 10.4KK14 pKa = 10.56GDD16 pKa = 3.48KK17 pKa = 10.69KK18 pKa = 10.8NVSFMDD24 pKa = 3.08WDD26 pKa = 4.02EE27 pKa = 4.36PPSYY31 pKa = 10.83SDD33 pKa = 3.79SRR35 pKa = 11.84YY36 pKa = 9.61GCYY39 pKa = 9.75PSAPLFGVDD48 pKa = 4.99EE49 pKa = 4.31MMEE52 pKa = 4.2TLPTLGIQSLKK63 pKa = 10.18IQYY66 pKa = 9.51KK67 pKa = 10.24CSLQVRR73 pKa = 11.84AEE75 pKa = 4.25TPFTSFNDD83 pKa = 3.44AARR86 pKa = 11.84CISLWEE92 pKa = 3.83TDD94 pKa = 3.25YY95 pKa = 11.39RR96 pKa = 11.84GYY98 pKa = 11.0AGKK101 pKa = 10.57KK102 pKa = 7.81PFYY105 pKa = 9.88RR106 pKa = 11.84LLMLIATKK114 pKa = 10.44KK115 pKa = 10.22LRR117 pKa = 11.84AAPMSLMDD125 pKa = 4.22GNRR128 pKa = 11.84PEE130 pKa = 4.34YY131 pKa = 10.89SSMIQGQSIVHH142 pKa = 6.45HH143 pKa = 6.18SLGVIPPMMHH153 pKa = 5.84VPEE156 pKa = 4.47TFTRR160 pKa = 11.84EE161 pKa = 3.74WNLLTNKK168 pKa = 9.96GMITAQIWLGITDD181 pKa = 4.04VVDD184 pKa = 4.68DD185 pKa = 5.35LNPLINPALFSDD197 pKa = 4.33EE198 pKa = 5.29KK199 pKa = 11.35EE200 pKa = 4.07MTLTSQMFGLEE211 pKa = 3.99LKK213 pKa = 10.28KK214 pKa = 11.04RR215 pKa = 11.84NDD217 pKa = 3.59NTWLISKK224 pKa = 10.14SYY226 pKa = 10.85

Molecular weight: 25.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3554

226

2093

710.8

80.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.952 ± 0.461

1.716 ± 0.288

5.937 ± 0.642

5.318 ± 0.327

3.995 ± 0.339

5.993 ± 0.394

2.279 ± 0.335

6.415 ± 0.554

6.528 ± 0.423

10.27 ± 0.626

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.926 ± 0.466

4.024 ± 0.335

4.53 ± 0.746

3.63 ± 0.276

5.121 ± 0.446

9.567 ± 0.765

5.656 ± 0.149

5.712 ± 0.696

1.97 ± 0.176

3.461 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski