Amino acid dipepetide frequency for Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.595AlaAla: 2.595 ± 0.099
0.369AlaCys: 0.369 ± 0.025
2.411AlaAsp: 2.411 ± 0.07
3.631AlaGlu: 3.631 ± 0.081
2.593AlaPhe: 2.593 ± 0.075
3.961AlaGly: 3.961 ± 0.102
0.786AlaHis: 0.786 ± 0.037
5.487AlaIle: 5.487 ± 0.104
4.101AlaLys: 4.101 ± 0.092
6.423AlaLeu: 6.423 ± 0.115
1.356AlaMet: 1.356 ± 0.046
2.142AlaAsn: 2.142 ± 0.06
1.609AlaPro: 1.609 ± 0.053
1.282AlaGln: 1.282 ± 0.047
2.521AlaArg: 2.521 ± 0.076
3.954AlaSer: 3.954 ± 0.086
2.949AlaThr: 2.949 ± 0.08
4.383AlaVal: 4.383 ± 0.095
0.497AlaTrp: 0.497 ± 0.032
2.66AlaTyr: 2.66 ± 0.073
0.0AlaXaa: 0.0 ± 0.0
Cys
0.239CysAla: 0.239 ± 0.022
0.074CysCys: 0.074 ± 0.013
0.296CysAsp: 0.296 ± 0.021
0.397CysGlu: 0.397 ± 0.028
0.18CysPhe: 0.18 ± 0.014
0.657CysGly: 0.657 ± 0.038
0.108CysHis: 0.108 ± 0.013
0.407CysIle: 0.407 ± 0.025
0.497CysLys: 0.497 ± 0.028
0.416CysLeu: 0.416 ± 0.025
0.139CysMet: 0.139 ± 0.014
0.312CysAsn: 0.312 ± 0.021
0.424CysPro: 0.424 ± 0.028
0.116CysGln: 0.116 ± 0.012
0.228CysArg: 0.228 ± 0.019
0.37CysSer: 0.37 ± 0.023
0.283CysThr: 0.283 ± 0.023
0.366CysVal: 0.366 ± 0.03
0.065CysTrp: 0.065 ± 0.011
0.237CysTyr: 0.237 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
2.418AspAla: 2.418 ± 0.067
0.296AspCys: 0.296 ± 0.022
2.114AspAsp: 2.114 ± 0.071
3.964AspGlu: 3.964 ± 0.085
2.226AspPhe: 2.226 ± 0.056
2.774AspGly: 2.774 ± 0.063
0.609AspHis: 0.609 ± 0.035
4.726AspIle: 4.726 ± 0.096
3.951AspLys: 3.951 ± 0.087
5.146AspLeu: 5.146 ± 0.097
1.131AspMet: 1.131 ± 0.04
2.002AspAsn: 2.002 ± 0.052
2.247AspPro: 2.247 ± 0.065
0.991AspGln: 0.991 ± 0.039
2.09AspArg: 2.09 ± 0.061
2.72AspSer: 2.72 ± 0.068
1.986AspThr: 1.986 ± 0.058
4.049AspVal: 4.049 ± 0.08
0.581AspTrp: 0.581 ± 0.031
2.57AspTyr: 2.57 ± 0.071
0.0AspXaa: 0.0 ± 0.0
Glu
3.908GluAla: 3.908 ± 0.09
0.437GluCys: 0.437 ± 0.029
3.552GluAsp: 3.552 ± 0.085
6.321GluGlu: 6.321 ± 0.131
2.593GluPhe: 2.593 ± 0.07
4.128GluGly: 4.128 ± 0.092
0.704GluHis: 0.704 ± 0.037
7.025GluIle: 7.025 ± 0.119
5.842GluLys: 5.842 ± 0.122
7.171GluLeu: 7.171 ± 0.119
1.53GluMet: 1.53 ± 0.049
3.461GluAsn: 3.461 ± 0.079
1.766GluPro: 1.766 ± 0.055
1.315GluGln: 1.315 ± 0.044
3.427GluArg: 3.427 ± 0.089
3.32GluSer: 3.32 ± 0.074
2.63GluThr: 2.63 ± 0.07
5.391GluVal: 5.391 ± 0.097
0.677GluTrp: 0.677 ± 0.033
2.904GluTyr: 2.904 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
2.234PheAla: 2.234 ± 0.059
0.228PheCys: 0.228 ± 0.021
2.166PheAsp: 2.166 ± 0.061
2.321PheGlu: 2.321 ± 0.071
1.897PhePhe: 1.897 ± 0.068
2.783PheGly: 2.783 ± 0.075
0.688PheHis: 0.688 ± 0.038
3.623PheIle: 3.623 ± 0.091
2.693PheLys: 2.693 ± 0.07
4.703PheLeu: 4.703 ± 0.109
1.003PheMet: 1.003 ± 0.048
2.298PheAsn: 2.298 ± 0.059
1.843PhePro: 1.843 ± 0.057
0.937PheGln: 0.937 ± 0.041
1.786PheArg: 1.786 ± 0.053
3.712PheSer: 3.712 ± 0.082
2.424PheThr: 2.424 ± 0.069
2.897PheVal: 2.897 ± 0.062
0.437PheTrp: 0.437 ± 0.029
2.125PheTyr: 2.125 ± 0.059
0.0PheXaa: 0.0 ± 0.0
Gly
3.646GlyAla: 3.646 ± 0.093
0.396GlyCys: 0.396 ± 0.03
3.041GlyAsp: 3.041 ± 0.071
4.34GlyGlu: 4.34 ± 0.086
3.272GlyPhe: 3.272 ± 0.076
4.666GlyGly: 4.666 ± 0.113
0.96GlyHis: 0.96 ± 0.039
6.807GlyIle: 6.807 ± 0.107
5.62GlyLys: 5.62 ± 0.093
6.521GlyLeu: 6.521 ± 0.124
1.478GlyMet: 1.478 ± 0.048
3.0GlyAsn: 3.0 ± 0.069
1.728GlyPro: 1.728 ± 0.052
1.329GlyGln: 1.329 ± 0.047
2.908GlyArg: 2.908 ± 0.076
4.372GlySer: 4.372 ± 0.091
3.136GlyThr: 3.136 ± 0.081
5.646GlyVal: 5.646 ± 0.101
0.775GlyTrp: 0.775 ± 0.032
3.473GlyTyr: 3.473 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
0.758HisAla: 0.758 ± 0.033
0.085HisCys: 0.085 ± 0.011
0.641HisAsp: 0.641 ± 0.034
0.88HisGlu: 0.88 ± 0.039
0.579HisPhe: 0.579 ± 0.029
1.036HisGly: 1.036 ± 0.04
0.266HisHis: 0.266 ± 0.023
1.022HisIle: 1.022 ± 0.041
0.796HisLys: 0.796 ± 0.035
1.252HisLeu: 1.252 ± 0.05
0.266HisMet: 0.266 ± 0.02
0.559HisAsn: 0.559 ± 0.031
0.736HisPro: 0.736 ± 0.036
0.274HisGln: 0.274 ± 0.021
0.536HisArg: 0.536 ± 0.03
0.864HisSer: 0.864 ± 0.035
0.578HisThr: 0.578 ± 0.033
0.992HisVal: 0.992 ± 0.045
0.146HisTrp: 0.146 ± 0.014
0.604HisTyr: 0.604 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.739IleAla: 5.739 ± 0.107
0.445IleCys: 0.445 ± 0.028
4.728IleAsp: 4.728 ± 0.096
5.522IleGlu: 5.522 ± 0.103
3.679IlePhe: 3.679 ± 0.092
6.184IleGly: 6.184 ± 0.12
1.18IleHis: 1.18 ± 0.043
7.812IleIle: 7.812 ± 0.122
6.423IleLys: 6.423 ± 0.107
9.117IleLeu: 9.117 ± 0.164
1.831IleMet: 1.831 ± 0.051
4.532IleAsn: 4.532 ± 0.092
4.18IlePro: 4.18 ± 0.088
2.089IleGln: 2.089 ± 0.054
4.245IleArg: 4.245 ± 0.091
7.282IleSer: 7.282 ± 0.126
4.739IleThr: 4.739 ± 0.095
6.696IleVal: 6.696 ± 0.101
0.652IleTrp: 0.652 ± 0.035
3.924IleTyr: 3.924 ± 0.088
0.0IleXaa: 0.0 ± 0.0
Lys
3.951LysAla: 3.951 ± 0.092
0.502LysCys: 0.502 ± 0.03
4.267LysAsp: 4.267 ± 0.091
6.407LysGlu: 6.407 ± 0.125
2.758LysPhe: 2.758 ± 0.065
4.935LysGly: 4.935 ± 0.081
0.777LysHis: 0.777 ± 0.032
7.223LysIle: 7.223 ± 0.113
5.454LysLys: 5.454 ± 0.119
7.592LysLeu: 7.592 ± 0.13
1.753LysMet: 1.753 ± 0.056
3.173LysAsn: 3.173 ± 0.08
2.695LysPro: 2.695 ± 0.071
1.541LysGln: 1.541 ± 0.052
3.9LysArg: 3.9 ± 0.097
4.07LysSer: 4.07 ± 0.082
3.057LysThr: 3.057 ± 0.078
6.385LysVal: 6.385 ± 0.105
0.775LysTrp: 0.775 ± 0.036
3.459LysTyr: 3.459 ± 0.077
0.0LysXaa: 0.0 ± 0.0
Leu
6.448LeuAla: 6.448 ± 0.107
0.521LeuCys: 0.521 ± 0.032
4.943LeuAsp: 4.943 ± 0.096
6.22LeuGlu: 6.22 ± 0.12
4.188LeuPhe: 4.188 ± 0.115
7.443LeuGly: 7.443 ± 0.127
1.282LeuHis: 1.282 ± 0.045
8.665LeuIle: 8.665 ± 0.148
7.673LeuLys: 7.673 ± 0.135
10.002LeuLeu: 10.002 ± 0.163
2.236LeuMet: 2.236 ± 0.066
5.106LeuAsn: 5.106 ± 0.1
4.192LeuPro: 4.192 ± 0.07
2.309LeuGln: 2.309 ± 0.061
5.027LeuArg: 5.027 ± 0.086
8.413LeuSer: 8.413 ± 0.134
5.218LeuThr: 5.218 ± 0.093
7.563LeuVal: 7.563 ± 0.116
0.853LeuTrp: 0.853 ± 0.041
4.15LeuTyr: 4.15 ± 0.082
0.0LeuXaa: 0.0 ± 0.0
Met
1.381MetAla: 1.381 ± 0.055
0.109MetCys: 0.109 ± 0.013
1.046MetAsp: 1.046 ± 0.041
1.525MetGlu: 1.525 ± 0.049
0.802MetPhe: 0.802 ± 0.035
1.524MetGly: 1.524 ± 0.05
0.264MetHis: 0.264 ± 0.019
1.97MetIle: 1.97 ± 0.052
1.945MetLys: 1.945 ± 0.053
1.97MetLeu: 1.97 ± 0.059
0.448MetMet: 0.448 ± 0.028
1.078MetAsn: 1.078 ± 0.038
0.832MetPro: 0.832 ± 0.034
0.445MetGln: 0.445 ± 0.029
1.245MetArg: 1.245 ± 0.046
1.76MetSer: 1.76 ± 0.05
1.022MetThr: 1.022 ± 0.045
1.434MetVal: 1.434 ± 0.048
0.255MetTrp: 0.255 ± 0.018
0.815MetTyr: 0.815 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
2.718AsnAla: 2.718 ± 0.061
0.348AsnCys: 0.348 ± 0.028
2.095AsnAsp: 2.095 ± 0.055
3.106AsnGlu: 3.106 ± 0.079
2.062AsnPhe: 2.062 ± 0.068
3.271AsnGly: 3.271 ± 0.1
0.492AsnHis: 0.492 ± 0.03
4.155AsnIle: 4.155 ± 0.098
3.25AsnLys: 3.25 ± 0.082
4.839AsnLeu: 4.839 ± 0.093
0.981AsnMet: 0.981 ± 0.039
2.388AsnAsn: 2.388 ± 0.072
2.361AsnPro: 2.361 ± 0.074
1.236AsnGln: 1.236 ± 0.05
1.866AsnArg: 1.866 ± 0.052
3.519AsnSer: 3.519 ± 0.104
2.37AsnThr: 2.37 ± 0.073
4.206AsnVal: 4.206 ± 0.082
0.489AsnTrp: 0.489 ± 0.028
2.473AsnTyr: 2.473 ± 0.076
0.0AsnXaa: 0.0 ± 0.0
Pro
1.869ProAla: 1.869 ± 0.059
0.201ProCys: 0.201 ± 0.02
1.896ProAsp: 1.896 ± 0.062
2.753ProGlu: 2.753 ± 0.067
1.894ProPhe: 1.894 ± 0.053
2.446ProGly: 2.446 ± 0.065
0.652ProHis: 0.652 ± 0.03
3.215ProIle: 3.215 ± 0.074
2.563ProLys: 2.563 ± 0.065
4.169ProLeu: 4.169 ± 0.077
0.758ProMet: 0.758 ± 0.031
1.916ProAsn: 1.916 ± 0.061
1.791ProPro: 1.791 ± 0.07
1.149ProGln: 1.149 ± 0.05
1.402ProArg: 1.402 ± 0.049
3.017ProSer: 3.017 ± 0.071
2.029ProThr: 2.029 ± 0.056
3.0ProVal: 3.0 ± 0.075
0.453ProTrp: 0.453 ± 0.028
2.101ProTyr: 2.101 ± 0.062
0.0ProXaa: 0.0 ± 0.0
Gln
1.41GlnAla: 1.41 ± 0.05
0.103GlnCys: 0.103 ± 0.013
1.07GlnAsp: 1.07 ± 0.044
1.543GlnGlu: 1.543 ± 0.047
0.926GlnPhe: 0.926 ± 0.036
1.619GlnGly: 1.619 ± 0.054
0.264GlnHis: 0.264 ± 0.021
2.029GlnIle: 2.029 ± 0.055
1.65GlnLys: 1.65 ± 0.051
2.46GlnLeu: 2.46 ± 0.073
0.568GlnMet: 0.568 ± 0.029
1.161GlnAsn: 1.161 ± 0.05
0.778GlnPro: 0.778 ± 0.036
0.797GlnGln: 0.797 ± 0.044
1.041GlnArg: 1.041 ± 0.046
1.313GlnSer: 1.313 ± 0.051
1.06GlnThr: 1.06 ± 0.047
1.815GlnVal: 1.815 ± 0.051
0.229GlnTrp: 0.229 ± 0.017
0.935GlnTyr: 0.935 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
2.557ArgAla: 2.557 ± 0.068
0.255ArgCys: 0.255 ± 0.021
2.51ArgAsp: 2.51 ± 0.061
3.938ArgGlu: 3.938 ± 0.091
1.88ArgPhe: 1.88 ± 0.058
3.035ArgGly: 3.035 ± 0.073
0.506ArgHis: 0.506 ± 0.03
4.079ArgIle: 4.079 ± 0.086
3.804ArgLys: 3.804 ± 0.088
4.532ArgLeu: 4.532 ± 0.092
0.951ArgMet: 0.951 ± 0.042
2.109ArgAsn: 2.109 ± 0.056
1.51ArgPro: 1.51 ± 0.048
1.003ArgGln: 1.003 ± 0.045
2.826ArgArg: 2.826 ± 0.067
2.46ArgSer: 2.46 ± 0.059
1.793ArgThr: 1.793 ± 0.051
3.676ArgVal: 3.676 ± 0.091
0.432ArgTrp: 0.432 ± 0.026
1.899ArgTyr: 1.899 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
3.571SerAla: 3.571 ± 0.108
0.364SerCys: 0.364 ± 0.022
2.889SerAsp: 2.889 ± 0.064
4.01SerGlu: 4.01 ± 0.082
3.22SerPhe: 3.22 ± 0.083
4.521SerGly: 4.521 ± 0.099
0.896SerHis: 0.896 ± 0.038
6.215SerIle: 6.215 ± 0.131
5.228SerLys: 5.228 ± 0.102
8.086SerLeu: 8.086 ± 0.14
1.639SerMet: 1.639 ± 0.052
3.29SerAsn: 3.29 ± 0.089
3.071SerPro: 3.071 ± 0.072
1.908SerGln: 1.908 ± 0.065
2.934SerArg: 2.934 ± 0.082
5.818SerSer: 5.818 ± 0.163
3.73SerThr: 3.73 ± 0.099
4.764SerVal: 4.764 ± 0.09
0.734SerTrp: 0.734 ± 0.036
3.092SerTyr: 3.092 ± 0.083
0.0SerXaa: 0.0 ± 0.0
Thr
2.987ThrAla: 2.987 ± 0.091
0.263ThrCys: 0.263 ± 0.019
1.945ThrAsp: 1.945 ± 0.053
2.603ThrGlu: 2.603 ± 0.062
2.228ThrPhe: 2.228 ± 0.059
3.62ThrGly: 3.62 ± 0.087
0.68ThrHis: 0.68 ± 0.034
4.367ThrIle: 4.367 ± 0.082
2.97ThrLys: 2.97 ± 0.075
5.036ThrLeu: 5.036 ± 0.084
1.03ThrMet: 1.03 ± 0.036
2.233ThrAsn: 2.233 ± 0.077
2.419ThrPro: 2.419 ± 0.055
1.229ThrGln: 1.229 ± 0.049
1.951ThrArg: 1.951 ± 0.054
3.625ThrSer: 3.625 ± 0.085
2.984ThrThr: 2.984 ± 0.097
3.908ThrVal: 3.908 ± 0.083
0.511ThrTrp: 0.511 ± 0.035
2.293ThrTyr: 2.293 ± 0.071
0.0ThrXaa: 0.0 ± 0.0
Val
4.28ValAla: 4.28 ± 0.096
0.392ValCys: 0.392 ± 0.027
4.103ValAsp: 4.103 ± 0.081
5.315ValGlu: 5.315 ± 0.081
3.142ValPhe: 3.142 ± 0.079
4.711ValGly: 4.711 ± 0.08
0.953ValHis: 0.953 ± 0.042
7.538ValIle: 7.538 ± 0.099
6.53ValLys: 6.53 ± 0.098
7.209ValLeu: 7.209 ± 0.111
1.657ValMet: 1.657 ± 0.054
4.302ValAsn: 4.302 ± 0.094
2.82ValPro: 2.82 ± 0.068
1.498ValGln: 1.498 ± 0.048
3.503ValArg: 3.503 ± 0.084
5.412ValSer: 5.412 ± 0.098
3.962ValThr: 3.962 ± 0.081
6.416ValVal: 6.416 ± 0.118
0.635ValTrp: 0.635 ± 0.034
3.587ValTyr: 3.587 ± 0.078
0.0ValXaa: 0.0 ± 0.0
Trp
0.505TrpAla: 0.505 ± 0.024
0.059TrpCys: 0.059 ± 0.01
0.565TrpAsp: 0.565 ± 0.032
0.578TrpGlu: 0.578 ± 0.033
0.464TrpPhe: 0.464 ± 0.031
0.685TrpGly: 0.685 ± 0.034
0.147TrpHis: 0.147 ± 0.016
0.954TrpIle: 0.954 ± 0.041
0.793TrpLys: 0.793 ± 0.04
1.014TrpLeu: 1.014 ± 0.045
0.226TrpMet: 0.226 ± 0.018
0.549TrpAsn: 0.549 ± 0.031
0.247TrpPro: 0.247 ± 0.018
0.204TrpGln: 0.204 ± 0.019
0.541TrpArg: 0.541 ± 0.029
0.622TrpSer: 0.622 ± 0.033
0.437TrpThr: 0.437 ± 0.022
0.695TrpVal: 0.695 ± 0.035
0.158TrpTrp: 0.158 ± 0.013
0.44TrpTyr: 0.44 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.497TyrAla: 2.497 ± 0.061
0.366TyrCys: 0.366 ± 0.027
2.312TyrAsp: 2.312 ± 0.06
2.728TyrGlu: 2.728 ± 0.079
2.215TyrPhe: 2.215 ± 0.058
3.117TyrGly: 3.117 ± 0.069
0.653TyrHis: 0.653 ± 0.032
3.677TyrIle: 3.677 ± 0.075
2.891TyrLys: 2.891 ± 0.066
4.796TyrLeu: 4.796 ± 0.103
0.886TyrMet: 0.886 ± 0.034
2.616TyrAsn: 2.616 ± 0.067
2.025TyrPro: 2.025 ± 0.063
1.122TyrGln: 1.122 ± 0.04
1.775TyrArg: 1.775 ± 0.051
3.356TyrSer: 3.356 ± 0.074
2.497TyrThr: 2.497 ± 0.066
3.684TyrVal: 3.684 ± 0.073
0.528TyrTrp: 0.528 ± 0.03
2.532TyrTyr: 2.532 ± 0.081
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2222 proteins (631988 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski