Bacteroides coprophilus CAG:333

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2352 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6TJR5|R6TJR5_9BACE DUF4105 domain-containing protein OS=Bacteroides coprophilus CAG:333 OX=1263041 GN=BN612_00957 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.22IKK4 pKa = 10.61GYY6 pKa = 10.29ILGISVAMASLLVSSCDD23 pKa = 3.35TDD25 pKa = 3.45NLTDD29 pKa = 4.0VYY31 pKa = 10.95QSSTIGATFITASQSVSFPSSGYY54 pKa = 9.71EE55 pKa = 3.92GFDD58 pKa = 3.45VEE60 pKa = 4.69VVRR63 pKa = 11.84AQTEE67 pKa = 3.95APATIKK73 pKa = 9.41VTSAVLLDD81 pKa = 3.91ADD83 pKa = 4.76GNALPLPSTIQVPSEE98 pKa = 4.12VNFEE102 pKa = 4.23AGEE105 pKa = 4.37GRR107 pKa = 11.84SSLHH111 pKa = 5.91VSVGDD116 pKa = 3.43ITPGEE121 pKa = 4.38TYY123 pKa = 10.62QLAITLDD130 pKa = 3.67EE131 pKa = 4.28TVAPVDD137 pKa = 3.24ATMTKK142 pKa = 9.78IISIFRR148 pKa = 11.84DD149 pKa = 3.37YY150 pKa = 10.92TYY152 pKa = 10.91TSLGTGTLNSTFFEE166 pKa = 4.37ATGTAEE172 pKa = 3.95IQKK175 pKa = 10.51ADD177 pKa = 3.25QTTWYY182 pKa = 9.71KK183 pKa = 11.03AIGLYY188 pKa = 10.41EE189 pKa = 4.21EE190 pKa = 5.69GYY192 pKa = 10.95DD193 pKa = 4.52IIFKK197 pKa = 10.13VAQDD201 pKa = 3.77GKK203 pKa = 9.07TVTVDD208 pKa = 3.27QQAAASDD215 pKa = 3.24ISGYY219 pKa = 8.37GTLYY223 pKa = 10.51VAGSGTLEE231 pKa = 3.97NGVITVEE238 pKa = 4.66LEE240 pKa = 4.08FTVSAGSFGSCTEE253 pKa = 3.87EE254 pKa = 5.47LILPAANN261 pKa = 3.27

Molecular weight: 27.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6SLB9|R6SLB9_9BACE Uncharacterized protein OS=Bacteroides coprophilus CAG:333 OX=1263041 GN=BN612_00764 PE=4 SV=1
MM1 pKa = 7.35HH2 pKa = 6.65VRR4 pKa = 11.84HH5 pKa = 5.58TVEE8 pKa = 3.47MHH10 pKa = 4.77QVILAVINDD19 pKa = 3.94RR20 pKa = 11.84RR21 pKa = 11.84DD22 pKa = 3.38HH23 pKa = 6.56LPVLRR28 pKa = 11.84RR29 pKa = 11.84QTAEE33 pKa = 3.44QEE35 pKa = 3.72MRR37 pKa = 11.84IPVLFQIIRR46 pKa = 11.84HH47 pKa = 5.93LLRR50 pKa = 11.84LAQIRR55 pKa = 11.84TLLRR59 pKa = 11.84EE60 pKa = 3.68MPVRR64 pKa = 11.84FKK66 pKa = 10.96IEE68 pKa = 4.06IPQHH72 pKa = 4.81TVSCPALRR80 pKa = 11.84RR81 pKa = 11.84QFLIQLPVVVRR92 pKa = 11.84TAHH95 pKa = 6.09QYY97 pKa = 10.38HH98 pKa = 6.74IPQVTALRR106 pKa = 11.84TVRR109 pKa = 11.84LDD111 pKa = 3.06QHH113 pKa = 5.55TQAQPEE119 pKa = 4.2HH120 pKa = 6.77RR121 pKa = 11.84HH122 pKa = 5.24TDD124 pKa = 3.05QHH126 pKa = 5.68RR127 pKa = 11.84QQVRR131 pKa = 11.84QKK133 pKa = 8.12QLRR136 pKa = 11.84PGIHH140 pKa = 6.85ISSQLQDD147 pKa = 4.97SPAQDD152 pKa = 3.65CKK154 pKa = 9.84EE155 pKa = 3.91HH156 pKa = 6.97HH157 pKa = 6.85ILGHH161 pKa = 6.1TGQHH165 pKa = 7.51LIRR168 pKa = 11.84EE169 pKa = 4.52HH170 pKa = 6.49LPSVKK175 pKa = 10.13NYY177 pKa = 9.6PRR179 pKa = 11.84MHH181 pKa = 6.77RR182 pKa = 11.84TQQVTQHH189 pKa = 5.07QRR191 pKa = 11.84RR192 pKa = 11.84HH193 pKa = 4.78NLEE196 pKa = 4.15LRR198 pKa = 11.84CLEE201 pKa = 4.23CLLPGRR207 pKa = 11.84ILCPMIKK214 pKa = 9.75SVKK217 pKa = 9.5QSAYY221 pKa = 9.36DD222 pKa = 3.35TGIAGNDD229 pKa = 3.07KK230 pKa = 10.66CVNKK234 pKa = 10.04PVIRR238 pKa = 11.84LCNSSFHH245 pKa = 7.15DD246 pKa = 3.51LVLFNMFFYY255 pKa = 10.84KK256 pKa = 10.49KK257 pKa = 9.4FGYY260 pKa = 10.45FIFPLPRR267 pKa = 11.84EE268 pKa = 3.88LHH270 pKa = 6.36AFQLPITLLISRR282 pKa = 11.84II283 pKa = 3.86

Molecular weight: 33.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2352

0

2352

846506

30

1536

359.9

40.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.404 ± 0.044

1.343 ± 0.017

5.36 ± 0.03

6.696 ± 0.046

4.511 ± 0.037

7.043 ± 0.04

2.016 ± 0.021

6.476 ± 0.043

6.006 ± 0.036

9.557 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.82 ± 0.023

4.614 ± 0.043

3.953 ± 0.028

3.71 ± 0.03

5.031 ± 0.039

5.894 ± 0.039

5.434 ± 0.03

6.582 ± 0.044

1.278 ± 0.021

4.272 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski