Gordonia phage Gsput1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gesputvirus; Gordonia virus Gsput1

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3T710|A0A0E3T710_9CAUD Uncharacterized protein OS=Gordonia phage Gsput1 OX=1622193 GN=Gsput1_69 PE=4 SV=1
MM1 pKa = 7.52TFTLRR6 pKa = 11.84LFGVPVISVEE16 pKa = 3.71IGTPDD21 pKa = 3.21HH22 pKa = 5.3EE23 pKa = 4.73HH24 pKa = 6.84YY25 pKa = 11.07YY26 pKa = 10.69EE27 pKa = 5.13GGEE30 pKa = 3.91IEE32 pKa = 4.66GPGFALTSDD41 pKa = 3.69GGALGFGWSDD51 pKa = 3.36EE52 pKa = 4.18

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3T689|A0A0E3T689_9CAUD Uncharacterized protein OS=Gordonia phage Gsput1 OX=1622193 GN=Gsput1_36 PE=4 SV=1
MM1 pKa = 7.23TGVIVSVFALSTLTTASVVRR21 pKa = 11.84LVCVHH26 pKa = 5.83KK27 pKa = 10.64RR28 pKa = 11.84SLRR31 pKa = 11.84ARR33 pKa = 11.84QLTISVVCFAIACALLIVARR53 pKa = 11.84YY54 pKa = 8.65GPAWLNPYY62 pKa = 9.44GVASLARR69 pKa = 11.84NLIAAFGYY77 pKa = 9.85VAIAKK82 pKa = 9.07HH83 pKa = 5.72ALISLRR89 pKa = 11.84RR90 pKa = 11.84ADD92 pKa = 5.33KK93 pKa = 10.48LANCNRR99 pKa = 11.84HH100 pKa = 5.19RR101 pKa = 11.84VVVCLVLVGSWVAASLPRR119 pKa = 11.84VLEE122 pKa = 4.21ANEE125 pKa = 4.14IPFGIPSNPAMSLHH139 pKa = 7.02WIAVAYY145 pKa = 10.11VAMGAWLVLALAVFEE160 pKa = 4.7GLDD163 pKa = 3.68GAGEE167 pKa = 4.06RR168 pKa = 11.84SRR170 pKa = 11.84LVLLTWFAAAAVGIVANVKK189 pKa = 10.08RR190 pKa = 11.84VINAISPWEE199 pKa = 4.01TSWGGSWEE207 pKa = 4.21LSFTAGGLLACGVIMSAMLTRR228 pKa = 11.84KK229 pKa = 9.21RR230 pKa = 11.84AAVVRR235 pKa = 11.84AAVV238 pKa = 3.15

Molecular weight: 25.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

13423

39

1431

189.1

20.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.009 ± 0.579

0.887 ± 0.171

6.772 ± 0.265

6.183 ± 0.355

2.913 ± 0.157

9.879 ± 0.425

2.049 ± 0.232

3.516 ± 0.156

3.814 ± 0.282

7.621 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.944 ± 0.126

3.01 ± 0.222

4.313 ± 0.286

3.092 ± 0.171

7.368 ± 0.481

5.58 ± 0.4

5.61 ± 0.271

8.761 ± 0.359

2.101 ± 0.125

2.578 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski