Escherichia phage ZG49

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; Escherichia virus ZG49

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291NXB2|A0A291NXB2_9CAUD Uncharacterized protein OS=Escherichia phage ZG49 OX=1897641 PE=4 SV=1
MM1 pKa = 7.86KK2 pKa = 10.12IPEE5 pKa = 4.24SLKK8 pKa = 10.68EE9 pKa = 3.9LVLEE13 pKa = 4.01MGEE16 pKa = 3.85FDD18 pKa = 4.48KK19 pKa = 11.18EE20 pKa = 3.87HH21 pKa = 6.61RR22 pKa = 11.84KK23 pKa = 10.27YY24 pKa = 11.3GEE26 pKa = 5.1FYY28 pKa = 10.88ASWSFTLTEE37 pKa = 4.56KK38 pKa = 10.84DD39 pKa = 3.3VEE41 pKa = 4.64AYY43 pKa = 10.22AEE45 pKa = 4.41SPAEE49 pKa = 4.11VYY51 pKa = 8.98EE52 pKa = 3.74WRR54 pKa = 11.84PFIGLTIVASGMWDD68 pKa = 3.17DD69 pKa = 4.97GYY71 pKa = 11.59GSEE74 pKa = 4.43VNDD77 pKa = 3.31WDD79 pKa = 4.35VYY81 pKa = 10.08RR82 pKa = 11.84QVEE85 pKa = 4.47KK86 pKa = 9.73EE87 pKa = 4.28TEE89 pKa = 3.88MSSNFWSLMSHH100 pKa = 7.05LNSEE104 pKa = 4.86DD105 pKa = 3.36NDD107 pKa = 3.96MAHH110 pKa = 6.77EE111 pKa = 4.68FANKK115 pKa = 7.84WLKK118 pKa = 10.92PEE120 pKa = 3.94VTLEE124 pKa = 4.17RR125 pKa = 11.84IEE127 pKa = 4.17EE128 pKa = 4.27PKK130 pKa = 10.68VSLMPATVAMATGGLDD146 pKa = 4.29DD147 pKa = 5.82DD148 pKa = 5.47FEE150 pKa = 4.65

Molecular weight: 17.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D3QLT1|A0A2D3QLT1_9CAUD Uncharacterized protein OS=Escherichia phage ZG49 OX=1897641 PE=4 SV=1
MM1 pKa = 7.23QNTPRR6 pKa = 11.84ILRR9 pKa = 11.84HH10 pKa = 5.79RR11 pKa = 11.84RR12 pKa = 11.84IQGLLSKK19 pKa = 10.55LKK21 pKa = 9.35STKK24 pKa = 10.06CPNGFRR30 pKa = 11.84TKK32 pKa = 10.36CPRR35 pKa = 11.84WKK37 pKa = 10.44AALIGLLLTLSLTISGCASEE57 pKa = 4.82SNLRR61 pKa = 11.84VEE63 pKa = 4.23PRR65 pKa = 11.84KK66 pKa = 9.19VTVDD70 pKa = 3.39ASLMISPNLTNEE82 pKa = 4.13MLNVLSPSEE91 pKa = 3.92

Molecular weight: 10.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

11761

47

1295

267.3

29.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.741 ± 0.559

0.867 ± 0.167

6.59 ± 0.278

6.564 ± 0.362

3.699 ± 0.229

8.018 ± 0.381

1.513 ± 0.176

4.94 ± 0.179

6.403 ± 0.379

7.984 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.925 ± 0.197

4.277 ± 0.275

3.835 ± 0.208

3.733 ± 0.448

5.408 ± 0.199

6.904 ± 0.428

5.816 ± 0.243

7.057 ± 0.311

1.437 ± 0.168

3.291 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski