Defluviitalea raffinosedens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Defluviitaleaceae; Defluviitalea

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2804 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7C8LF30|A0A7C8LF30_9FIRM HD domain-containing protein OS=Defluviitalea raffinosedens OX=1450156 GN=GND95_06680 PE=4 SV=1
MM1 pKa = 7.3ATLFKK6 pKa = 10.78LVISAEE12 pKa = 4.19TTTNVSTNPDD22 pKa = 3.18VEE24 pKa = 4.53RR25 pKa = 11.84YY26 pKa = 9.03FYY28 pKa = 11.2SVDD31 pKa = 3.17PAHH34 pKa = 7.13RR35 pKa = 11.84VGSTLEE41 pKa = 3.83IPATAFVDD49 pKa = 4.05DD50 pKa = 4.16TDD52 pKa = 4.2TPVTTITEE60 pKa = 5.1AIAGNGYY67 pKa = 9.95YY68 pKa = 10.83LLFINGVLQQSDD80 pKa = 4.94LYY82 pKa = 10.14TISDD86 pKa = 3.96TQVVITDD93 pKa = 3.88AASIPDD99 pKa = 3.77GAVITLVVTNFVPDD113 pKa = 3.86ADD115 pKa = 4.02STTTIITT122 pKa = 3.57

Molecular weight: 13.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7C8LFP4|A0A7C8LFP4_9FIRM YihY family inner membrane protein OS=Defluviitalea raffinosedens OX=1450156 GN=GND95_05835 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.07KK9 pKa = 8.55RR10 pKa = 11.84QRR12 pKa = 11.84QKK14 pKa = 9.41EE15 pKa = 3.76HH16 pKa = 6.27GFRR19 pKa = 11.84KK20 pKa = 9.76RR21 pKa = 11.84MKK23 pKa = 8.31TASGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2804

0

2804

894548

26

2207

319.0

35.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.407 ± 0.044

1.024 ± 0.019

5.335 ± 0.031

7.781 ± 0.05

4.206 ± 0.033

6.593 ± 0.044

1.741 ± 0.022

9.337 ± 0.048

7.953 ± 0.038

9.147 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.792 ± 0.022

5.05 ± 0.034

3.294 ± 0.026

3.24 ± 0.029

3.757 ± 0.029

5.922 ± 0.034

5.048 ± 0.034

6.555 ± 0.04

0.81 ± 0.014

4.007 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski