Neisseria shayeganii 871

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Neisseria; Neisseria shayeganii

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2530 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4CH73|G4CH73_9NEIS Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme OS=Neisseria shayeganii 871 OX=1032488 GN=glnE PE=3 SV=1
MM1 pKa = 7.11STPDD5 pKa = 3.4EE6 pKa = 4.35SPIIFTEE13 pKa = 4.44SCCTKK18 pKa = 10.44VADD21 pKa = 6.42LIAEE25 pKa = 4.23EE26 pKa = 4.56NNPDD30 pKa = 3.05LKK32 pKa = 11.19LRR34 pKa = 11.84VFVNGGGCSGFQYY47 pKa = 10.94GFTFDD52 pKa = 5.34EE53 pKa = 4.5ITNDD57 pKa = 4.42DD58 pKa = 4.01DD59 pKa = 4.99FQIEE63 pKa = 4.34KK64 pKa = 10.53NGLVFLIDD72 pKa = 3.64PMSYY76 pKa = 10.14QYY78 pKa = 11.58LVGAEE83 pKa = 3.68IDD85 pKa = 3.94YY86 pKa = 10.52TEE88 pKa = 4.7SLHH91 pKa = 6.92GSQFVIRR98 pKa = 11.84NPNAATTCGCGSSFSVV114 pKa = 3.54

Molecular weight: 12.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4CHH9|G4CHH9_9NEIS Histidinol-phosphate aminotransferase OS=Neisseria shayeganii 871 OX=1032488 GN=hisC PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVTKK11 pKa = 10.56RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.89GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2530

0

2530

666819

32

2826

263.6

29.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.742 ± 0.08

1.123 ± 0.016

5.046 ± 0.036

5.711 ± 0.051

3.852 ± 0.04

7.611 ± 0.046

2.565 ± 0.031

4.919 ± 0.042

3.988 ± 0.047

10.841 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.503 ± 0.026

3.348 ± 0.035

4.802 ± 0.037

4.692 ± 0.049

6.394 ± 0.048

5.265 ± 0.038

4.652 ± 0.034

6.722 ± 0.048

1.428 ± 0.025

2.796 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski