Methylophaga lonarensis MPL

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Methylophaga; Methylophaga lonarensis

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2536 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M7PDE0|M7PDE0_9GAMM 50S ribosomal protein L35 OS=Methylophaga lonarensis MPL OX=1286106 GN=rpmI PE=3 SV=1
MM1 pKa = 7.54SEE3 pKa = 5.12LYY5 pKa = 10.39FPSLSPEE12 pKa = 3.76NMAGDD17 pKa = 4.39GPSTQAEE24 pKa = 4.32LQGALCGLLCMNAKK38 pKa = 10.36ANRR41 pKa = 11.84TDD43 pKa = 2.81WFRR46 pKa = 11.84QLFEE50 pKa = 5.09DD51 pKa = 3.95FHH53 pKa = 8.34PGEE56 pKa = 4.65DD57 pKa = 3.96EE58 pKa = 4.75INALTQLFDD67 pKa = 3.48QTIQALNSPEE77 pKa = 4.2YY78 pKa = 10.31DD79 pKa = 3.34FKK81 pKa = 11.52LAFPEE86 pKa = 4.71DD87 pKa = 4.22SFPLASRR94 pKa = 11.84LIAMADD100 pKa = 3.0WCQGLIYY107 pKa = 10.59GLGASGLTDD116 pKa = 3.69EE117 pKa = 5.39TDD119 pKa = 3.49LSADD123 pKa = 3.46SQEE126 pKa = 4.57YY127 pKa = 9.6IVDD130 pKa = 4.22VIKK133 pKa = 10.58ISQIADD139 pKa = 3.14VDD141 pKa = 4.19LSNADD146 pKa = 3.16EE147 pKa = 4.92DD148 pKa = 3.92EE149 pKa = 4.92ANFEE153 pKa = 4.26EE154 pKa = 4.52LTEE157 pKa = 4.0YY158 pKa = 11.01LRR160 pKa = 11.84MGLFVLYY167 pKa = 10.76SEE169 pKa = 5.25LQPEE173 pKa = 5.01DD174 pKa = 3.59PTQSITEE181 pKa = 4.15HH182 pKa = 6.18

Molecular weight: 20.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M7PI62|M7PI62_9GAMM Adenylate cyclase OS=Methylophaga lonarensis MPL OX=1286106 GN=cyaA PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 6.05GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.28TVGGRR28 pKa = 11.84RR29 pKa = 11.84VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84AKK41 pKa = 10.1LTVV44 pKa = 3.04

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2536

0

2536

774044

23

1971

305.2

33.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.47 ± 0.045

0.947 ± 0.018

5.719 ± 0.037

6.115 ± 0.053

3.859 ± 0.035

6.839 ± 0.043

2.471 ± 0.026

6.129 ± 0.035

3.832 ± 0.038

11.006 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.757 ± 0.026

3.614 ± 0.029

4.187 ± 0.026

5.254 ± 0.044

5.722 ± 0.039

6.242 ± 0.041

5.061 ± 0.035

6.814 ± 0.039

1.289 ± 0.024

2.667 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski