Brockia lithotrophica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Brockia

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1680 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A660L1P7|A0A660L1P7_9THEO Glutamine--fructose-6-phosphate aminotransferase [isomerizing] OS=Brockia lithotrophica OX=933949 GN=glmS PE=3 SV=1
MM1 pKa = 7.72IYY3 pKa = 10.35AGYY6 pKa = 9.95SIEE9 pKa = 5.66RR10 pKa = 11.84GDD12 pKa = 3.61DD13 pKa = 3.49CEE15 pKa = 5.63AEE17 pKa = 4.26IFLEE21 pKa = 4.21AAGDD25 pKa = 3.84ILLLLSMVLPVGEE38 pKa = 4.04ATYY41 pKa = 10.84TNDD44 pKa = 2.83VPGRR48 pKa = 11.84LTSASYY54 pKa = 10.64EE55 pKa = 3.63IVQNNNPHH63 pKa = 6.65LNNPHH68 pKa = 6.52LL69 pKa = 4.15

Molecular weight: 7.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A660KXD8|A0A660KXD8_9THEO Amino acid/amide ABC transporter substrate-binding protein (HAAT family) OS=Brockia lithotrophica OX=933949 GN=C7438_1175 PE=3 SV=1
MM1 pKa = 7.89PKK3 pKa = 9.5MKK5 pKa = 9.95SHH7 pKa = 6.86RR8 pKa = 11.84GAMKK12 pKa = 9.78RR13 pKa = 11.84FKK15 pKa = 10.06RR16 pKa = 11.84TGSGKK21 pKa = 9.36IVHH24 pKa = 6.08WRR26 pKa = 11.84NNRR29 pKa = 11.84SHH31 pKa = 7.09LNEE34 pKa = 4.04HH35 pKa = 7.08KK36 pKa = 10.34SRR38 pKa = 11.84KK39 pKa = 7.99RR40 pKa = 11.84LRR42 pKa = 11.84RR43 pKa = 11.84LRR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 9.69AVFGKK52 pKa = 7.92TQAKK56 pKa = 9.54RR57 pKa = 11.84ILRR60 pKa = 11.84AAPYY64 pKa = 10.4LKK66 pKa = 10.69

Molecular weight: 7.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1680

0

1680

543884

26

1889

323.7

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.963 ± 0.084

0.445 ± 0.015

4.651 ± 0.039

8.06 ± 0.066

4.179 ± 0.036

8.623 ± 0.049

1.914 ± 0.021

3.934 ± 0.046

2.916 ± 0.05

11.695 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.585 ± 0.023

1.811 ± 0.03

5.852 ± 0.046

2.078 ± 0.027

9.193 ± 0.074

4.682 ± 0.04

4.526 ± 0.034

8.926 ± 0.047

1.207 ± 0.02

2.759 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski