Gordonia phage Mulch

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Betterkatzvirus; unclassified Betterkatzvirus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411AWU5|A0A411AWU5_9CAUD Membrane protein OS=Gordonia phage Mulch OX=2500788 GN=54 PE=4 SV=1
MM1 pKa = 7.37TNHH4 pKa = 6.9EE5 pKa = 4.12GHH7 pKa = 6.32QNVTPDD13 pKa = 3.21VSAVTLTTTYY23 pKa = 8.2TQPADD28 pKa = 3.58GWPLAALDD36 pKa = 3.8AFGIMFAPEE45 pKa = 4.33QITITASADD54 pKa = 3.31TTGTVEE60 pKa = 4.05VLPVVSVAGHH70 pKa = 6.6IGDD73 pKa = 3.83QAVTTSANVGDD84 pKa = 4.24LPAYY88 pKa = 9.39VLEE91 pKa = 4.52LVEE94 pKa = 4.18HH95 pKa = 6.67AKK97 pKa = 10.4LAAAHH102 pKa = 6.45ALAVAA107 pKa = 4.67

Molecular weight: 10.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411AWR8|A0A411AWR8_9CAUD Tail assembly chaperone OS=Gordonia phage Mulch OX=2500788 GN=19 PE=4 SV=1
MM1 pKa = 7.41TGRR4 pKa = 11.84PRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84IYY10 pKa = 10.54RR11 pKa = 11.84LEE13 pKa = 3.76SHH15 pKa = 6.76GDD17 pKa = 3.17VFKK20 pKa = 10.01FTRR23 pKa = 11.84CARR26 pKa = 11.84CGKK29 pKa = 10.13AIAYY33 pKa = 7.46LAHH36 pKa = 5.23VQVWVHH42 pKa = 5.13VVSVYY47 pKa = 11.11DD48 pKa = 4.25HH49 pKa = 6.63IAVVPRR55 pKa = 11.84FVTDD59 pKa = 3.3VPEE62 pKa = 4.26RR63 pKa = 11.84FAGVLNQAVRR73 pKa = 11.84PP74 pKa = 4.03

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

15907

37

1594

215.0

23.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.957 ± 0.457

0.893 ± 0.148

7.148 ± 0.279

5.256 ± 0.27

2.559 ± 0.154

9.323 ± 0.46

2.037 ± 0.182

4.017 ± 0.198

2.785 ± 0.266

7.99 ± 0.268

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.735 ± 0.102

2.716 ± 0.163

5.84 ± 0.342

3.024 ± 0.192

7.538 ± 0.38

5.318 ± 0.266

6.978 ± 0.248

7.971 ± 0.27

1.993 ± 0.12

1.924 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski