Actinomarinicola tropica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Acidimicrobiia; Acidimicrobiales; Iamiaceae; Actinomarinicola

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2RDX8|A0A5Q2RDX8_9ACTN (2R 3S)-2-methylisocitrate dehydratase OS=Actinomarinicola tropica OX=2789776 GN=GH723_08280 PE=4 SV=1
MM1 pKa = 7.47GGAADD6 pKa = 4.76DD7 pKa = 4.37GRR9 pKa = 11.84GGAPEE14 pKa = 4.32AVVEE18 pKa = 4.25LQPAEE23 pKa = 4.28PGRR26 pKa = 11.84GLPRR30 pKa = 11.84WAPTAIFAALVLLIGVVVAAQDD52 pKa = 3.86PGADD56 pKa = 4.74DD57 pKa = 5.27PGTDD61 pKa = 4.77DD62 pKa = 6.57DD63 pKa = 4.51LTEE66 pKa = 4.62DD67 pKa = 4.88LAFEE71 pKa = 4.95LDD73 pKa = 3.17GATAVGGPRR82 pKa = 11.84DD83 pKa = 3.7GLDD86 pKa = 3.31SLRR89 pKa = 11.84LPVQATPAQGLVDD102 pKa = 4.15GQTVTVSGSGFPPSRR117 pKa = 11.84NLGIVMCTPLGPSSIGGVDD136 pKa = 3.13NCQISPYY143 pKa = 9.67TAVTSDD149 pKa = 3.04AQGNFSAEE157 pKa = 4.15HH158 pKa = 5.5PVRR161 pKa = 11.84RR162 pKa = 11.84YY163 pKa = 7.79VTLANGVHH171 pKa = 7.22DD172 pKa = 4.77CAQAPPEE179 pKa = 4.71GSTHH183 pKa = 5.14TCVVAVGAIDD193 pKa = 5.98DD194 pKa = 4.12YY195 pKa = 11.53DD196 pKa = 3.7QSGTIGVHH204 pKa = 6.51FDD206 pKa = 3.43PDD208 pKa = 3.91VPGTPPLSIHH218 pKa = 6.7MDD220 pKa = 3.3PQGPVSVGDD229 pKa = 3.71TLTVTVEE236 pKa = 3.77NAAPGSSWWVDD247 pKa = 3.0VCGQGEE253 pKa = 4.48GGEE256 pKa = 4.25VDD258 pKa = 3.21QWGHH262 pKa = 4.77PTYY265 pKa = 11.15VSACASGAEE274 pKa = 4.41AYY276 pKa = 7.65TFCAVGGEE284 pKa = 4.21PACASPGGTEE294 pKa = 4.31VVAGPDD300 pKa = 3.36GTATFSLPAPASIAGADD317 pKa = 3.57GVVDD321 pKa = 4.07CRR323 pKa = 11.84SLTSFCEE330 pKa = 4.06VVVRR334 pKa = 11.84DD335 pKa = 3.43GDD337 pKa = 3.95TNGRR341 pKa = 11.84SFPVLLHH348 pKa = 6.03EE349 pKa = 4.74VADD352 pKa = 4.22ASSPPSTAVPPHH364 pKa = 5.42VTDD367 pKa = 3.29TTMSTEE373 pKa = 4.09TTTIGPDD380 pKa = 2.98EE381 pKa = 4.56GVTTEE386 pKa = 4.25TTVMPTTTVPGDD398 pKa = 3.22

Molecular weight: 39.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2RND4|A0A5Q2RND4_9ACTN Uncharacterized protein OS=Actinomarinicola tropica OX=2789776 GN=GH723_12920 PE=4 SV=1
MM1 pKa = 7.57GSLIKK6 pKa = 10.25KK7 pKa = 8.32RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.27RR11 pKa = 11.84MRR13 pKa = 11.84KK14 pKa = 8.3KK15 pKa = 10.03KK16 pKa = 9.06HH17 pKa = 5.6RR18 pKa = 11.84KK19 pKa = 5.5MLKK22 pKa = 7.83RR23 pKa = 11.84TRR25 pKa = 11.84FQRR28 pKa = 11.84RR29 pKa = 11.84AKK31 pKa = 10.52GKK33 pKa = 9.8

Molecular weight: 4.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3559

0

3559

1144291

33

2937

321.5

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.251 ± 0.057

0.716 ± 0.012

7.074 ± 0.036

6.223 ± 0.039

2.703 ± 0.027

9.296 ± 0.036

2.358 ± 0.022

3.622 ± 0.023

1.335 ± 0.026

10.088 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.809 ± 0.02

1.507 ± 0.022

5.937 ± 0.03

2.391 ± 0.017

8.331 ± 0.048

4.86 ± 0.027

5.654 ± 0.029

9.668 ± 0.043

1.523 ± 0.015

1.654 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski