Arthrobacter phage LittleTokyo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9J712|A0A6B9J712_9CAUD Helix-turn-helix DNA-binding domain protein OS=Arthrobacter phage LittleTokyo OX=2686081 GN=39 PE=4 SV=1
MM1 pKa = 7.85SDD3 pKa = 3.34LDD5 pKa = 3.89EE6 pKa = 5.21PGKK9 pKa = 10.89VLLNPEE15 pKa = 4.19CRR17 pKa = 11.84DD18 pKa = 3.36GKK20 pKa = 10.22HH21 pKa = 5.97RR22 pKa = 11.84NCNGEE27 pKa = 4.35GWDD30 pKa = 3.58TWADD34 pKa = 3.7DD35 pKa = 4.46ATDD38 pKa = 4.49CPCEE42 pKa = 3.99CHH44 pKa = 6.75

Molecular weight: 4.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9J7Y7|A0A6B9J7Y7_9CAUD Uncharacterized protein OS=Arthrobacter phage LittleTokyo OX=2686081 GN=66 PE=4 SV=1
MM1 pKa = 7.64IGVLLRR7 pKa = 11.84ALAVLLGNKK16 pKa = 9.75LCDD19 pKa = 3.56VGLHH23 pKa = 5.05KK24 pKa = 10.42WSRR27 pKa = 11.84SGVGDD32 pKa = 3.35FCFRR36 pKa = 11.84PACNGRR42 pKa = 11.84TWRR45 pKa = 11.84RR46 pKa = 11.84VVVAGPRR53 pKa = 11.84INVYY57 pKa = 8.68GVRR60 pKa = 11.84EE61 pKa = 4.01CRR63 pKa = 11.84PRR65 pKa = 11.84IVRR68 pKa = 11.84AIAWGDD74 pKa = 3.41KK75 pKa = 10.65FNDD78 pKa = 3.6EE79 pKa = 4.53GGSS82 pKa = 3.45

Molecular weight: 9.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

12325

30

787

168.8

18.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.527 ± 0.38

1.201 ± 0.158

5.785 ± 0.185

6.548 ± 0.319

2.564 ± 0.178

9.574 ± 0.483

1.428 ± 0.145

3.335 ± 0.169

3.546 ± 0.237

9.014 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.053 ± 0.141

2.264 ± 0.161

5.785 ± 0.284

2.88 ± 0.175

7.675 ± 0.408

5.615 ± 0.269

5.274 ± 0.346

8.552 ± 0.283

2.377 ± 0.158

2.004 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski