Rachiplusia ou multiple nucleopolyhedrovirus (strain R1) (RoMNPV)

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus; Autographa californica multiple nucleopolyhedrovirus; Rachiplusia ou MNPV

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 149 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8B9J8|Q8B9J8_NPVR1 Uncharacterized protein OS=Rachiplusia ou multiple nucleopolyhedrovirus (strain R1) OX=654904 PE=4 SV=1
MM1 pKa = 6.79TNAWFATDD9 pKa = 3.81VNLINCILKK18 pKa = 10.85DD19 pKa = 3.73NLFLVDD25 pKa = 3.73NNYY28 pKa = 10.24IILNVFDD35 pKa = 4.36QEE37 pKa = 4.17TDD39 pKa = 3.26QVRR42 pKa = 11.84PLCLGEE48 pKa = 4.24INALQTDD55 pKa = 4.11AAAQADD61 pKa = 3.89AMLDD65 pKa = 3.42TSSTSEE71 pKa = 4.07LQSNASTT78 pKa = 3.69

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8B9D2|Q8B9D2_NPVR1 Gp16 OS=Rachiplusia ou multiple nucleopolyhedrovirus (strain R1) OX=654904 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 8.07PTNNVMFDD10 pKa = 3.7DD11 pKa = 6.11ASVLWIDD18 pKa = 3.72TDD20 pKa = 4.41YY21 pKa = 11.02IYY23 pKa = 11.21QNLKK27 pKa = 9.57MPLQAFQQLLFTIPSKK43 pKa = 10.03HH44 pKa = 5.88RR45 pKa = 11.84KK46 pKa = 7.82MINDD50 pKa = 3.71AGGSCHH56 pKa = 5.81NTVKK60 pKa = 10.97YY61 pKa = 9.87MVDD64 pKa = 2.77IYY66 pKa = 11.18GAAVLVLRR74 pKa = 11.84TPCSFADD81 pKa = 3.49QLLSTFIANNYY92 pKa = 8.53LCYY95 pKa = 9.83FYY97 pKa = 10.79RR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84SRR103 pKa = 11.84SRR105 pKa = 11.84SRR107 pKa = 11.84SRR109 pKa = 11.84SRR111 pKa = 11.84SPHH114 pKa = 5.16CRR116 pKa = 11.84PRR118 pKa = 11.84SRR120 pKa = 11.84SPHH123 pKa = 5.28CRR125 pKa = 11.84PRR127 pKa = 11.84SRR129 pKa = 11.84SRR131 pKa = 11.84SRR133 pKa = 11.84SRR135 pKa = 11.84SRR137 pKa = 11.84SRR139 pKa = 11.84SSSPRR144 pKa = 11.84RR145 pKa = 11.84GRR147 pKa = 11.84RR148 pKa = 11.84QIFEE152 pKa = 3.99ALEE155 pKa = 4.39KK156 pKa = 10.16IRR158 pKa = 11.84HH159 pKa = 5.1QNDD162 pKa = 2.9MLMSNVNQINLNQTNQFLEE181 pKa = 4.64LSNMMTGVRR190 pKa = 11.84NQNVQLLAALEE201 pKa = 4.14TAKK204 pKa = 10.87DD205 pKa = 3.83VILTRR210 pKa = 11.84LNTLLAEE217 pKa = 4.59ITDD220 pKa = 4.18SLPDD224 pKa = 3.73LTSMLDD230 pKa = 3.42KK231 pKa = 10.81LAEE234 pKa = 4.0QLLDD238 pKa = 4.78AINTVQQTLRR248 pKa = 11.84NEE250 pKa = 4.37LNNTNSILTNLASSVTNINGTLNNLLAAIEE280 pKa = 3.93NLIGGGGGGGNFNEE294 pKa = 5.08ADD296 pKa = 3.97RR297 pKa = 11.84QKK299 pKa = 11.49LDD301 pKa = 3.68LVYY304 pKa = 10.68TLVNEE309 pKa = 4.64IKK311 pKa = 10.78NILTGTLTKK320 pKa = 10.49KK321 pKa = 10.64

Molecular weight: 36.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

149

0

149

41291

53

1221

277.1

32.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.035 ± 0.175

2.56 ± 0.161

5.946 ± 0.152

5.253 ± 0.134

5.037 ± 0.134

3.238 ± 0.173

2.231 ± 0.11

6.788 ± 0.133

7.314 ± 0.271

9.399 ± 0.161

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.078

8.179 ± 0.196

3.851 ± 0.26

3.768 ± 0.133

4.48 ± 0.169

6.285 ± 0.162

5.662 ± 0.151

6.488 ± 0.129

0.77 ± 0.069

4.994 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski