Salmonella phage SPN3UB

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6WRT9|H6WRT9_9CAUD Uncharacterized protein OS=Salmonella phage SPN3UB OX=1147140 GN=SPN3UB_0012 PE=4 SV=1
MM1 pKa = 7.97SEE3 pKa = 3.8IARR6 pKa = 11.84WSYY9 pKa = 11.19TNVATIYY16 pKa = 10.38PRR18 pKa = 11.84VYY20 pKa = 10.98DD21 pKa = 3.78DD22 pKa = 4.88WNNAWTSGTPYY33 pKa = 10.73LIDD36 pKa = 3.89CTWTANNEE44 pKa = 4.22VAVDD48 pKa = 3.7ASGKK52 pKa = 10.13EE53 pKa = 3.95FTTNLIFFTEE63 pKa = 4.66LKK65 pKa = 10.65CNGVDD70 pKa = 2.9ATMPLRR76 pKa = 11.84DD77 pKa = 3.42WYY79 pKa = 10.02IARR82 pKa = 11.84GDD84 pKa = 3.41TTAQVDD90 pKa = 3.92PLKK93 pKa = 11.07AGANVIKK100 pKa = 10.54AVTEE104 pKa = 3.73WDD106 pKa = 3.2MSPFGEE112 pKa = 4.22EE113 pKa = 3.62PDD115 pKa = 3.92YY116 pKa = 11.42KK117 pKa = 10.71ILTT120 pKa = 3.93

Molecular weight: 13.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6WRZ0|H6WRZ0_9CAUD Uncharacterized protein OS=Salmonella phage SPN3UB OX=1147140 GN=SPN3UB_0063 PE=4 SV=1
MM1 pKa = 7.63AKK3 pKa = 10.3LPRR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 10.07CKK10 pKa = 9.87VCRR13 pKa = 11.84EE14 pKa = 4.21WFHH17 pKa = 6.31PAYY20 pKa = 11.0SNVVWCCPEE29 pKa = 3.87HH30 pKa = 5.92GAIYY34 pKa = 10.39ALKK37 pKa = 10.43LRR39 pKa = 11.84AKK41 pKa = 9.88EE42 pKa = 4.03KK43 pKa = 10.23IKK45 pKa = 10.76AAARR49 pKa = 11.84RR50 pKa = 11.84IKK52 pKa = 10.23EE53 pKa = 3.55KK54 pKa = 10.57HH55 pKa = 5.19QADD58 pKa = 3.36KK59 pKa = 11.46AEE61 pKa = 4.15RR62 pKa = 11.84QRR64 pKa = 11.84RR65 pKa = 11.84QAKK68 pKa = 9.87RR69 pKa = 11.84EE70 pKa = 4.06SFKK73 pKa = 10.94TKK75 pKa = 10.43AQWDD79 pKa = 4.07KK80 pKa = 10.78EE81 pKa = 4.01AQAAFNRR88 pKa = 11.84YY89 pKa = 8.21IRR91 pKa = 11.84IRR93 pKa = 11.84DD94 pKa = 3.43EE95 pKa = 4.25GKK97 pKa = 9.57PCISCDD103 pKa = 3.29APLVGKK109 pKa = 10.64SNFLTGSAIDD119 pKa = 3.6ASHH122 pKa = 5.58YY123 pKa = 9.46RR124 pKa = 11.84SRR126 pKa = 11.84GAASHH131 pKa = 6.67LKK133 pKa = 9.12FNVFNVHH140 pKa = 6.17SACTRR145 pKa = 11.84CNRR148 pKa = 11.84QLSGNAVEE156 pKa = 4.1YY157 pKa = 10.26RR158 pKa = 11.84IRR160 pKa = 11.84LIRR163 pKa = 11.84RR164 pKa = 11.84IGLEE168 pKa = 3.48RR169 pKa = 11.84VEE171 pKa = 4.98RR172 pKa = 11.84LEE174 pKa = 3.98SDD176 pKa = 3.4NVPRR180 pKa = 11.84RR181 pKa = 11.84FDD183 pKa = 3.03IPYY186 pKa = 9.69LKK188 pKa = 10.06RR189 pKa = 11.84IKK191 pKa = 10.78SIFTRR196 pKa = 11.84KK197 pKa = 9.21ARR199 pKa = 11.84ALEE202 pKa = 3.71KK203 pKa = 10.44RR204 pKa = 11.84RR205 pKa = 11.84ARR207 pKa = 11.84RR208 pKa = 11.84QDD210 pKa = 2.95NAAA213 pKa = 3.58

Molecular weight: 24.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

14246

48

1059

200.6

22.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.518 ± 0.427

1.207 ± 0.15

5.637 ± 0.128

6.676 ± 0.392

3.201 ± 0.168

6.914 ± 0.263

1.629 ± 0.156

5.798 ± 0.211

6.037 ± 0.288

7.665 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.513 ± 0.143

4.801 ± 0.236

4.156 ± 0.263

4.036 ± 0.237

6.079 ± 0.377

6.598 ± 0.287

5.981 ± 0.311

6.718 ± 0.29

1.657 ± 0.122

3.18 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski