Dickeya phage Luksen

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Aarhusvirus; Dickeya virus Luksen

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385IG82|A0A385IG82_9CAUD HNS binding protein OS=Dickeya phage Luksen OX=2320192 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.15PEE4 pKa = 4.89EE5 pKa = 4.03ILKK8 pKa = 8.1TLCDD12 pKa = 4.01ALTEE16 pKa = 4.31EE17 pKa = 4.79PWISEE22 pKa = 4.21LPDD25 pKa = 3.26EE26 pKa = 5.32LGFILVGNDD35 pKa = 2.73DD36 pKa = 3.8WVDD39 pKa = 3.23EE40 pKa = 4.37GKK42 pKa = 10.18YY43 pKa = 10.1QSLCSVVKK51 pKa = 10.65HH52 pKa = 5.84EE53 pKa = 4.12EE54 pKa = 3.68SGLYY58 pKa = 10.06FEE60 pKa = 5.46ATFSRR65 pKa = 11.84HH66 pKa = 4.33GDD68 pKa = 2.55HH69 pKa = 6.34WQGHH73 pKa = 4.44EE74 pKa = 4.2TEE76 pKa = 5.31FDD78 pKa = 3.61GACQVEE84 pKa = 4.81PVQVMVTQYY93 pKa = 10.94RR94 pKa = 11.84RR95 pKa = 11.84VSS97 pKa = 3.14

Molecular weight: 11.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385IGN6|A0A385IGN6_9CAUD Uncharacterized protein OS=Dickeya phage Luksen OX=2320192 PE=4 SV=1
MM1 pKa = 6.2VTVWDD6 pKa = 4.21TMSGTLRR13 pKa = 11.84LSWKK17 pKa = 9.85ALRR20 pKa = 11.84AKK22 pKa = 9.42MHH24 pKa = 5.84GRR26 pKa = 11.84PLCWRR31 pKa = 11.84NKK33 pKa = 6.06MTSKK37 pKa = 10.97SNSQNKK43 pKa = 10.01AGFIKK48 pKa = 10.0RR49 pKa = 11.84TWQRR53 pKa = 11.84TKK55 pKa = 10.99LIPLGLLMIYY65 pKa = 10.29VSLVTGCASGSQPVRR80 pKa = 11.84PLSPQVQVDD89 pKa = 3.65QALMVTPNFQEE100 pKa = 4.12TLLKK104 pKa = 10.16YY105 pKa = 10.77LSVNPDD111 pKa = 2.89VPMSGLNDD119 pKa = 3.6SKK121 pKa = 11.31RR122 pKa = 11.84PP123 pKa = 3.6

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12907

41

1320

243.5

27.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.173 ± 0.458

1.007 ± 0.165

6.097 ± 0.251

6.679 ± 0.404

4.006 ± 0.23

7.391 ± 0.381

1.883 ± 0.201

5.284 ± 0.242

6.64 ± 0.323

7.988 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.991 ± 0.152

4.687 ± 0.238

3.789 ± 0.195

4.23 ± 0.303

5.114 ± 0.193

6.392 ± 0.3

5.392 ± 0.268

6.81 ± 0.217

1.426 ± 0.15

3.022 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski