Firmicutes bacterium CAG:110

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5F079|R5F079_9FIRM Protein phosphatase 2C OS=Firmicutes bacterium CAG:110 OX=1263000 GN=BN466_00167 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.45KK3 pKa = 10.26SLTRR7 pKa = 11.84VLLACLLVLTVVFACGCSNTPDD29 pKa = 3.67PSASNWKK36 pKa = 7.78VTFYY40 pKa = 10.74EE41 pKa = 4.59GDD43 pKa = 3.47GTTVLQEE50 pKa = 4.01VEE52 pKa = 4.41VADD55 pKa = 4.41GEE57 pKa = 4.46PVAMPEE63 pKa = 4.15LTKK66 pKa = 10.48EE67 pKa = 4.29GYY69 pKa = 9.93IIEE72 pKa = 5.15GYY74 pKa = 10.04YY75 pKa = 8.98ATPALKK81 pKa = 10.78VEE83 pKa = 4.59FDD85 pKa = 3.36VTQPITEE92 pKa = 4.07DD93 pKa = 3.14TAIFVAWQSSVVDD106 pKa = 3.7EE107 pKa = 4.92RR108 pKa = 11.84PWMLAGSLAGYY119 pKa = 8.87PDD121 pKa = 3.92NAWGKK126 pKa = 9.21IWPQNDD132 pKa = 3.39YY133 pKa = 11.04LLQPMEE139 pKa = 4.49GEE141 pKa = 4.34FNTFYY146 pKa = 10.79IDD148 pKa = 3.34VNLYY152 pKa = 10.73AGDD155 pKa = 3.72AFKK158 pKa = 10.66IAVIGEE164 pKa = 4.4GYY166 pKa = 10.5VWDD169 pKa = 4.37NDD171 pKa = 3.35NSIDD175 pKa = 4.91ASHH178 pKa = 7.33LANKK182 pKa = 8.98TEE184 pKa = 4.07SAVLCSGEE192 pKa = 3.9NAFDD196 pKa = 3.31SGANIEE202 pKa = 4.11VLEE205 pKa = 4.63DD206 pKa = 3.21GKK208 pKa = 11.31YY209 pKa = 10.33RR210 pKa = 11.84LILKK214 pKa = 8.98TDD216 pKa = 3.39AEE218 pKa = 4.48TLSLCSLSYY227 pKa = 10.75EE228 pKa = 4.19RR229 pKa = 11.84LGDD232 pKa = 3.88ADD234 pKa = 4.13EE235 pKa = 5.14KK236 pKa = 10.97PVAEE240 pKa = 5.04LSYY243 pKa = 11.62DD244 pKa = 3.49MKK246 pKa = 11.07LWASFNDD253 pKa = 3.54WSGQDD258 pKa = 3.37MQRR261 pKa = 11.84NGDD264 pKa = 4.4DD265 pKa = 4.57LIWWCEE271 pKa = 3.3ADD273 pKa = 3.8VPAGGGEE280 pKa = 3.98FGIKK284 pKa = 8.69NAAMDD289 pKa = 3.79GWYY292 pKa = 10.48SSEE295 pKa = 3.99NNSKK299 pKa = 11.06NIVLEE304 pKa = 4.2EE305 pKa = 3.67GHH307 pKa = 6.4YY308 pKa = 10.03MFFIEE313 pKa = 5.72LEE315 pKa = 4.39VVDD318 pKa = 5.19GKK320 pKa = 10.09PVLKK324 pKa = 10.77DD325 pKa = 3.32EE326 pKa = 5.36IIAEE330 pKa = 4.13EE331 pKa = 3.7PAYY334 pKa = 10.6YY335 pKa = 10.58VVGTCGNAGWAADD348 pKa = 3.59ANAEE352 pKa = 3.91NTAYY356 pKa = 10.09KK357 pKa = 8.37MTEE360 pKa = 3.67QDD362 pKa = 2.96SKK364 pKa = 11.82YY365 pKa = 10.25VLTVTFTEE373 pKa = 4.91DD374 pKa = 3.13EE375 pKa = 4.44TVDD378 pKa = 3.02WAEE381 pKa = 3.81NKK383 pKa = 9.76VAFKK387 pKa = 10.64VAYY390 pKa = 9.49GCGGKK395 pKa = 9.28VANEE399 pKa = 3.65NWFGAEE405 pKa = 3.79GMDD408 pKa = 4.62NVTVDD413 pKa = 3.44PGTYY417 pKa = 9.98TITFDD422 pKa = 3.79PATGAVTVEE431 pKa = 4.02

Molecular weight: 47.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5EHD0|R5EHD0_9FIRM Putative membrane protein insertion efficiency factor OS=Firmicutes bacterium CAG:110 OX=1263000 GN=BN466_01037 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.26QPSKK9 pKa = 8.09RR10 pKa = 11.84HH11 pKa = 5.53RR12 pKa = 11.84SMVHH16 pKa = 5.93GFRR19 pKa = 11.84QRR21 pKa = 11.84MATSNGRR28 pKa = 11.84KK29 pKa = 8.67VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2311

0

2311

703987

30

2589

304.6

33.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.241 ± 0.049

1.761 ± 0.024

5.717 ± 0.044

6.471 ± 0.051

3.911 ± 0.038

7.815 ± 0.044

1.792 ± 0.025

5.94 ± 0.038

5.129 ± 0.049

9.558 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.937 ± 0.028

3.625 ± 0.029

4.031 ± 0.034

3.259 ± 0.029

5.229 ± 0.048

5.571 ± 0.041

5.838 ± 0.055

7.353 ± 0.044

1.088 ± 0.019

3.731 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski