Rummeliibacillus stabekisii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Rummeliibacillus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3235 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143H9V6|A0A143H9V6_9BACL Flagellar motor switch protein FliM OS=Rummeliibacillus stabekisii OX=241244 GN=fliM PE=3 SV=1
MM1 pKa = 7.73PKK3 pKa = 8.98YY4 pKa = 9.39TIVDD8 pKa = 3.64KK9 pKa = 8.25EE10 pKa = 4.35TCIACGACEE19 pKa = 4.36ASAPDD24 pKa = 3.14IFDD27 pKa = 3.61YY28 pKa = 11.32DD29 pKa = 3.77EE30 pKa = 4.72EE31 pKa = 6.37GISHH35 pKa = 7.33VIFDD39 pKa = 4.41DD40 pKa = 3.68NEE42 pKa = 3.92GRR44 pKa = 11.84VEE46 pKa = 4.09VPEE49 pKa = 3.99YY50 pKa = 10.87LLEE53 pKa = 4.54DD54 pKa = 4.03LEE56 pKa = 4.98DD57 pKa = 3.95AVEE60 pKa = 4.39GCPTDD65 pKa = 3.81SIKK68 pKa = 10.83VEE70 pKa = 4.23SEE72 pKa = 3.52PFFAVKK78 pKa = 10.2SS79 pKa = 3.8

Molecular weight: 8.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143HGN3|A0A143HGN3_9BACL Serine hydroxymethyltransferase OS=Rummeliibacillus stabekisii OX=241244 GN=glyA PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.66GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84KK29 pKa = 9.39VIAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3235

0

3235

927364

37

1781

286.7

32.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.172 ± 0.045

0.694 ± 0.013

5.117 ± 0.032

7.451 ± 0.057

4.431 ± 0.036

6.629 ± 0.044

2.086 ± 0.018

8.006 ± 0.047

7.385 ± 0.046

9.634 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.791 ± 0.022

4.524 ± 0.037

3.47 ± 0.026

3.848 ± 0.032

3.862 ± 0.031

5.986 ± 0.032

5.574 ± 0.031

6.793 ± 0.039

0.966 ± 0.014

3.581 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski